白奕天¹, 丁天扬¹, 李东东^1,2, 聂鸿涛^1,2, 闫喜武^1,2
1.大连海洋大学 水产与生命学院;2.大连海洋大学, 辽宁省贝类良种繁育工程技术研究中心

摘要:
为研究不同盐度对文蛤呼吸代谢的影响, 本实验设置5个盐度(‰)梯度(11、18、25、32、39), 检测不同盐度对文蛤(Meretrix meretrix)耗氧和排氨的影响, 以及文蛤的外套膜、鳃、肝胰腺三种组织中乳酸脱氢酶和Na₊/K₊-ATP酶活性的变化。结果表明: 随着盐度的不断升高, 文蛤耗氧率先升后降再升,在盐度18时达到最大值; 排氨率先升后降, 在盐度32时达到最大值。随着盐度不断升高和胁迫时间延长, 文蛤的肝胰腺中乳酸脱氢酶活力总体呈先升高后下降再升高的趋势(P<0.05), 酶活力在盐度39时为最高; 随着盐度不断升高和胁迫时间延长, 文蛤的外套膜中Na₊/K₊-ATP酶活力总体呈先下降再升高后下降的趋势(P<0.05), 在盐度32时为最高; 文蛤的外套膜和鳃中乳酸脱氢酶活力以及鳃和肝胰腺中Na₊/K₊-ATP酶活力受盐度影响不显著(P>0.05), 酶活力变化也多呈现“W”形的变化趋势。研究结果为文蛤的人工养殖提供参考。
关键词:文蛤(Meretrix meretrix)盐度耗氧率排氨率乳酸脱氢酶Na₊/K₊-ATP 酶
DOI：10.11759/hykx20160817002
分类号:
基金项目:现代农业产业技术体系建设专项(CARS-49); 辽宁省教育厅科学技术研究项目(QL201703); 大连市青年科技之星项目(2016RQ065)资助

Effects of different salinity on respiratory metabolism and enzyme activities of Meretrix meretrix
BAI Yi-tian,DING Tian-yang,LI Dong-dong,NIE Hong-tao,YAN Xi-wu
Abstract:
In order to study the effects of salinity on the respiratory metabolism of Meretrix meretrix, five salinity gradients (11‰, 18‰, 25‰, 32‰, and 39‰) were set up to investigate the effects of acute salinity change on the oxygen consumption and ammonia excretion of M. meretrix, and the activity of lactate dehydrogenase (LDH) and Na₊/K₊-ATPase in the mantle, gills and hepatopancreas of M. meretrix. The results showed that with the increase of salinity, the oxygen consumption rate was firstly increased, then decreased and then increased, and the maximum value reached at salinity of 18, and the ammonia excretion rate was first increased and then decreased, and the maximum reached at salinity of 32. As the salinity increases and the time of stress is prolonged, the activity of LDH in the hepatopancreas of M. meretrix was increased firstly, then decreased and then increased (P < 0.05), and the enzyme activity was highest in 39 salinity. With the increase of salinity and the prolongation of stress time, the ATP activity in the mantle of M. meretrix was decreased firstly, then increased and then decreased (P < 0.05), which was highest at 32 salinity. The activity of LDH in mantle and gills and the ATP activity in gills and hepatopancreas of M. meretrix were not significantly affected by salinity (P > 0.05). The change of enzyme activity also showed the trend of “W” shape. The results provided a reference for the artificial culture technique of M. meretrix.
Key words:Meretrix meretrixacute salinity changeoxygen consumption rateammonia excretion ratelactate dehydrogenase (LDH)Na₊/K₊-ATPase





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