李 莉^1,2, 李晓明¹, 李洪雷¹, Belma Konuklugil³, 李 鑫¹, 王斌贵¹
1.中国科学院 海洋研究所 实验海洋生物学重点实验室;2.中国科学院大学;3.Department of Pharmacognosy, Faculty of Pharmacy, Ankara University

摘要:
焦曲霉(Aspergillus ustus)TK-5是分离自土耳其海域海鞘(Pyura momus)新鲜组织中的一株内生真菌, 利用正相与反相硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析以及高效液相制备等色谱方法从其发酵产物中分离得到17 个化合物, 通过一维、二维核磁共振、质谱等技术鉴定了所有化合物的结构, 分别为血苋烷型倍半萜类化合物 strobilactone A(1), ustusolate E(2), ustusolate C(3), ustusolate D(4), 11-hydroustusolate E(5), 11, 6’-hydroustusolate E(6), (2’E, 4’E, 6’E)-6-(1’-carboxyocta-2’, 4’, 6’-triene)-11, 12-epoxy-9, 11-dihydroxydrim-7-ene(7), 12-hydroxy-6-epi-albrassitriol (8), ustusolate A (9), deoxyuvidin B (10)和二倍半萜类化合物 6-epi-ophiobolin G(11), (6α)-21, 21-O-dihydroophiobolin G(12), 6-epi-ophiobolin K(13), ophiobolin P(14) ophiobolin H(15), ophiobolin Q(16)及ophiobolin R(17)。活性筛选表明化合物2、6、7、9、11 和13对神经氨酸酶具有一定的抑制活性, 其半数抑制浓度(IC₅₀) 分别为31.8、37.3、28.4、36.8、46.6和37.6 μmol/L。
关键词:海鞘内生真菌焦曲霉萜类神经氨酸酶抑制活性
DOI：10.11759/hykx20180320002
分类号:
基金项目:国家自然科学基金委员会-山东省海洋科学研究中心联合基金(U1606403)

Chemical constituents of Aspergillus ustus TK-5, an endophytic fungus derived from the ascidian Herdmania momus
LI Li,LI Xiao-ming,LI Hong-lei,BELMA Konuklugil,LI Xin,WANG Bin-gui
Abstract:
Cultivation of the fungal strain Aspergillus ustus TK-5, an endophytic fungus which was isolated from the fresh tissue of ascidian Herdmania momus, resulted in the identification of 17 compounds. These compounds were isolated by a combination of silica gel, Sephadex LH-20, and Lobar LiChroprep RP-18 column chromatography as well as by preparative high-performance liquid chromatography (pHPLC). The structures were elucidated to be drimane sesquiterpenoids (1–10) and sesterterpenes (11–17) by analysis of their spectroscopic data, including strobilactone A (1), ustusolate E (2), ustusolate C (3), ustusolate D (4), 11-hydroustusolate E (5), 11, 6’- hydroustusolate E (6), (2’E, 4’E, 6’E)-6-(1’-carboxyocta-2’, 4’, 6’-triene)-11, 12-epoxy-9, 11-dihydroxydrim-7-ene (7), 12-hydroxy-6-epi-albrassitriol (8), ustusolate A (9), deoxyuvidin B (10), 6-epi-ophiobolin G (11), (6α)-21, 21-O-dihydroophiobolin G (12), 6-epi-ophiobolin K (13), ophiobolin P (14), ophiobolin H (15), ophiobolin Q (16), and ophiobolin R (17). Regarding to the neuraminidase inhibitory activity, compounds 2, 6, 7, 9, 11, and 13 were found to possess moderate activity, with IC₅₀ values of 31.8, 37.3, 28.4, 36.8, 46.6, and 37.6 μmol/L.
Key words:AscidiansEndophytic fungusAspergillus ustusTerpenoidsNeuraminidase inhibitory activity





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