李红艳¹, 王璇璇², 李 晓¹, 刘天红¹, 姜晓东¹, 孙元芹¹, 纪 蕾¹, 王 颖¹
1.山东省海洋生物研究院;2.中国海洋大学 海洋生命学院

摘要:
以鼠尾藻为原料, 采用有机溶剂法提取岩藻黄素。通过单因素实验, 分析了不同有机溶剂、料液比、温度及时间对岩藻黄素提取率的影响, 并通过响应面法对提取工艺进行优化。结果表明, 最佳的提取工艺为90%甲醇溶液, 料液比1︰33, 提取温度60℃, 提取时间3.25 h, 提取率达到1.368 mg/g。
关键词:鼠尾藻岩藻黄素提取响应面法
DOI：10.11759/hykx20171024001
分类号:
基金项目:海洋公益性行业科研专项经费项目(201505022-4)

Optimization of fucoxanthin extraction from Sargassum thunbergii
LI Hong-yan,WANG Xuan-xuan,LI Xiao,LIU Tian-hong,JIANG Xiao-dong,SUN Yuan-qin,JI Lei,WANG Ying
Abstract:
In this paper, the technology of extracting fucoxanthin from Sargassum thunbergii is studied. Single-factor experiments were carried out to investigate the effects of different solvent, solid-liquid ratio, extraction temperature, and extraction time on the fucoxanthin extraction yield. The extraction technology is optimized by the response surface methodology. The results show that the optimal parameters for extraction are as follows: 90% methanol, solid-liquid ratio of 1︰32.8, 60℃ extraction temperature, and 3.25 h extraction time. The yield rate of fucoxanthin is 1.368 mg/g under optimal conditions. This work provides a theoretical basis for raw material selection for fucoxanthin extraction and the further development and utilization of S. thunbergii.
Key words:Sargassum thunbergiifucoxanthinextractionresponse surface method





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