姓名：秦贞奎


学历/职称：博士/副教授，硕士生导师

联系方式：
电话：
邮箱：qinzk@ouc.edu.cn
地址：鱼山路5号中国海洋大学化学馆326

学习工作经历：
2018.01 - 至今，中国海洋大学，海洋生命学院，副教授
2016.01-2017.12，中国海洋大学，海洋生命学院，讲师
2011.08-2015.12，美国Auburn University，Fisheries and Allied Aquaculture，博士
2008.09-2011.06，中国海洋大学，海洋生物学，硕士
2004.09-2008.06，中国海洋大学，生物科学，学士
研究领域简介：
主要研究方向为海洋动物生殖繁育及胚胎发育过程中的分子机制，以栉孔扇贝和侏儒蛤等动物为研究对象，通过细胞遗传发育生物学等多种技术手段对其发育关键基因的功能及调控机制进行研究。

主讲课程：
本科生课程《发育生物学》，《发育生物学实验》
主要研究课题：
1.国家自然科学基金青年基金：栉孔扇贝Klf4基因在性别形成中的调控机制研究（**，2017.01-2019.12），主持。
2.山东省自然科学基金青年基金：栉孔扇贝Sox2基因在性别形成中的调控机制研究（ZR2016CQ24，2016.11-2019.06），主持。
3.中国海洋大学-Auburn University联合基金：通过高效转基因技术改变鲶鱼中去饱和酶通路以提高Omega-3 脂肪酸的合成（2017.01-2018.12），中方主持。
4.中国海洋大学专项基金：海洋贝类模式动物平台搭建及组学分析（，2017.05-2020.05），参与。
5. 国家重点研发计划蓝色粮仓科技创新重点专项（2018YFD**，2018.12-2022.12），参与。

代表性论文：
1. Xitan Hou, Maokai Wei, Qi Li, Tingting Zhang, Di Zhou, Dexu Kong, Yueyang Xie, Zhenkui Qin*, Zhifeng Zhang*. Transcriptome analysis on larval segment formation and secondary loss in the echiuran worm Urechis unicinctus. International Journal of Molecular Sciences, 2019, 20:1806.
2. Zhenkui Qin^?, Xueyu Li^?, Danwen Liu, Qing Wang, Li Lu, Zhifeng Zhang*. Analysis of chromosome karyotype and genome size in echiuran Urechis unicinctusDrasche, 1880 (Polychaeta, Urechidae). Comparative Cytogenetics, 2019, 13: 75-85.
3. Shaoshuai Liang, Danwen Liu, Xixi Li, Maokai Wei, Xiaohan Yu, Qi Li, Huixin Ma, Zhifeng Zhang*, Zhenkui Qin*. SOX2 participates in spermatogenesis of Zhikong scallop Chlamys farreri. Scientific Reports, 2019, 9: 76.
4.Zhenkui Qin^?, Yanjie Zhang^?, Huawei Mu, Zhifeng Zhang*, Jian-Wen Qiu*. The sperm proteome of the echiuran Urechis unicinctus(Annelida, Echiura). Proteomics, 2018, 18: **.
5. Yajiao Bai, Di Zhou, Maokai Wei, Yueyang Xie, Beibei Gao, Zhenkui Qin*, Zhifeng Zhang*. Identification of Reference Genes for Normalizing Quantitative Real-Time PCR in Urechis unicinctus. Journal of Ocean University of China, 2018, 17: 614-622.
6. Yang Dandan, Shaoshuai Liang, Qiankun Yang, Danwen Liu, Zhenkui Qin*, Zhang Zhifeng*. Expression characteristics and functional analysis of Krüppel-like factor 4 in adductor muscle and mantle of Zhikong scallop Chlamys farreri. Development Genes and Evolution. 2018, 1-9.
7. Yang Dandan, Zhang Zhifeng, Shaoshuai Liang, Qiankun Yang, Yingrui Wang, Zhenkui Qin*. A novel role of Krüppel-like factor 4 in Zhikong scallop Chlamys farreriduring spermatogenesis. PLoS ONE. 2017. 12: e**.
8. Ma Xiaoshi^?, Aichang Ji^?, Zhifeng Zhang, Dandan Yang, Shaoshuai, Liang, Yunhan Wang and Zhenkui Qin*. Piwi1is essential for gametogenesis in mollusk Chlamys farreri.Peer J. 2017. 5: e3412.
9. Shaoshuai Liang, Zhifeng Zhang, Dandan Yang, Yingying Chen, Zhenkui Qin*. Different expression of sox17gene during gametogenesis between scallop Chlamys farreriand vertebrates. Gene Expression Patterns. 2017. 25-26: 102-108.
10. Zhenkui Qin, Yun Li, Baofeng Su, Qi Cheng, Zhi Ye, D. A. Perera, M. Fobes, Mei Shang, R. A. Dunham*. Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation. Marine Biotechnology. 2016. 18: 255-263.
11. Xiaoyu Ma, Xiaolong Liu, Di Zhou, Yajiao Bai, Beibei Gao, Zhifeng Zhang*, Zhenkui Qin*. The NF- kB pathway participates in the response to sulfide stress in Urechis unicinctus. Fish & Shellfish Immunology. 2016. 58: 229-238.
12. Zhenkui Qin, Yun Li, Dapeng Sun, Mingyu Shao, Zhifeng Zhang*. Cloning and expression analysis of the vitellogenin gene in the scallop Chlamys farreriand the effects of estradiol-17β on its synthesis. Invertebrate Biology, 2012, 131: 312-321.
13. Mei Shang, Baofeng Su, Dayan Perera, Ahmed Alsaqufi, Elizabeth Lipke, Sehriban Cek, David Dunn, Zhenkui Qin, Eric Peatman, Rex Dunham*. Testicular germ line cell identification, isolation, and transplantation in two North American catfish species. Fish physiology and biochemistry, 2018, 44: 717-733.
14. Aichang Ji, Xueyu Li, Sha Fang, Zhenkui Qin, Changming Bai, Chongming Wang, Zhifeng Zhang*. Primary culture of Zhikong scallop Chlamys farrerihemocytes as an in vitro model for studying host-pathogen interactions. Diseases of aquatic organisms, 2017, 125: 217-226.
15. Nermeen Abass, Ahmed Alsaqufi, Nonkonzo Makubu, Ahmed Elaswad, Zhi Ye, Baofeng Su, Zhenkui Qin, Hanbo Li, Rex Dunham*. Genotype-environment interactions for growth and survival of channel catfish (Ictalurus punctatus), blue catfish (Ictalurus furcatus), and channel catfish, I. punctatus, ♀× blue catfish, I. furcatus, ♂ hybrid fry at varying levels of sodium chloride. Aquaculture, 2017, 471: 28-36.
16. Yujia Yang^?, Qiang Fu^?, Yang Liu, Xiaozhu Wang, Rex Dunham, Shikai Liu, Lisui Bao, Qifan Zeng, Tao Zhou, Ning Li, Zhenkui Qin, Chen Jiang, Dongya Gao, Zhanjiang Liu*. Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladder. Physiological genomics, 2017, 50: 67-76.
17. Sheng Dong, Dayan Perera, Khoi Vo, Ramjie Odin, Ahmed Alsaquifi, Michael Fobes, Ahmed Elaswad, Zhi Ye, Zhenkui Qin, Hanbo Li, Dan Zhang, Guyu Qin, David Drescher, Nermeen Abass, Louie Thompson, Rex Dunham*. Channel catfish Ictalurus punctatus strain comparison for induced ovulation in the early spawning season to produce channel catfish♀× blue catfish I. furcatus♂ hybrid catfish embryos. Aquaculture, 2017, 471: 185-189.
18. Yujia Yang, Qiang Fu, Tao Zhou, Yun Li, Shikai Liu, Qifan Zeng, Xiaozhu Wang, Yulin Jin, Changxu Tian, Zhenkui Qin, Rex Dunham, Zhanjiang Liu*. Analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection. Developmental & Comparative Immunology, 2017, 67: 464-470.
19. Xiaolong Liu, Zhenkui Qin, Xueyu Li, Xiaoyu Ma, Beibei Gao, Zhifeng Zhang. NF1, Sp1 and HSF1 are synergistically involved in sulfide-induced sqractivation in echiuran wormUrechis unicinctus. Aquatic Toxicology. 2016. 175: 232-240.
20. Litao Zhang, Xiaolong Liu, Zhenkui Qin, Jianguo Liu, Zhifeng Zhang*. Expression characteristics of sulfur dioxygenase and its function adaption to sulfide in echiuran worm Urechis unicinctus.Gene. 2016. 593: 334–341.
21. Zhanjiang Liu^?,*, Shikai Liu^?, Jun Yao^?, Lisui Bao^?, Jiaren Zhang^?, Yun Li^?, et al. The channel catfish genome sequence provides insights into the evolution of scale formation in teleosts. Nature communications, 2016, 7: 11757.
22. Qiang Fu, Yun Li, Yujia Yang, Chao Li, Jun Yao, Qifan Zeng, Zhenkui Qin, Shikai Liu, Daoji Li, Zhanjiang Liu*. Septin genes in channel catfish (Ictalurus punctatus) and their involvement in disease defense responses. Fish & shellfish immunology, 2016, 49: 110-121.
23. Yun Li^?, Shikai Liu^?, Zhenkui Qin, Jun Yao, Chen Jiang, Lin Song, Rex Dunham, Zhanjiang Liu*. The serpin superfamily in channel catfish: identification, phylogenetic analysis and expression profiling in mucosal tissues after bacterial infections. Developmental & Comparative Immunology, 2015, 49: 267-277.
24. Yun Li?, Shikai Liu?, Zhenkui Qin, Geoff Waldbieser, Ruijiang Wang, Luyang Sun, Lisui Bao, Roy Danzmann, Rex Dunham, Zhanjiang Liu*. Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish. DNA Research, 2015, 22: 39-52.
25. Shikai Liu^?, Yun Li^?, Zhenkui Qin, Xin Geng, Lisui Bao, Ludmilla Kaltenboeck, Huseyin Kucuktas, Rex Dunham, Zhanjiang Liu*. High-density interspecific genetic linkage mapping provides insights into genomic incompatibility between channel catfish and blue catfish. Animal genetics, 2016, 47: 81-90.
26. Qi Cheng^?, Baofeng Su^?, Zhenkui Qin, Chia-Chen Weng, Fang Yin, Yangen Zhou, Michael Fobes, Dayan Perera, Mei Shang, Fabio Soller, Zhiyi Shi, Allen Davis, Rex Dunham*. Interaction of diet and the masou salmon Δ5-desaturase transgene on Δ6-desaturase and stearoyl-CoA desaturase gene expression and N-3 fatty acid level in common carp (Cyprinus carpio). Transgenic research, 2014, 23: 729-742.
27. Mei Shang, Baofeng Su, Elizabeth Lipke, Dayan Perera, Chao Li, Zhenkui Qin, Yun Li, David Dunn, Sehriban Cek, Eric Peatman, Rex Dunham*. Spermatogonial stem cells specific marker identification in channel catfish, Ictalurus punctatusand blue catfish, I. furcatus. Fish physiology and biochemistry, 2015, 41: 1545-1556.
28. 梁少帅, 于潇含, 杨丹丹, 秦贞奎^*. 栉孔扇贝sox9基因的cDNA 克隆及其在不同发育时期性腺中的表达特征. 中国水产科学, 2017, 24: 1184-1192.
29. 李金龙, 秦贞奎, 史晓丽, 张志峰*. 铜离子对单环刺螠的毒性及对体壁抗氧化酶活性的影响. 海洋湖沼通报, 2012, (2): 77-82.
30. 秦贞奎, 刘建国, 许姗姗, 邵明瑜, 张志峰*. 栉孔扇贝核糖体蛋白L24全长cDNA序列的克隆及其性腺表达. 中国海洋大学学报, 2011, 41 (sup.,): 203-207.
参与著作：
1. Rex A. Dunham, Ahmed Elaswad, Zhenkui Qin. Gene Editing in Channel Catfish via Double Electroporation of Zinc-Finger Nucleases. In: Liu J. (eds) Zinc Finger Proteins. Methods in Molecular Biology, vol 1867. Humana Press, New York, NY. 2018: 201-214.
2. Yun Li, Shikai Liu, Ruijia Wang, Zhenkui Qinand Zhangjiang Liu. Bioinformatics Considerations and Approaches for High-Density Linkage Mapping in Aquaculture. In: Liu Z. (eds) Bioinformatics in Aquaculture: Principles and Methods. John Wiley & Sons Ltd, Hoboken, NJ. 2017: 356-379.