上海交通大学 Med-X研究院， 上海 200240
出版日期:2018-03-01发布日期:2018-03-01Establishment of an In Vitro Cell Heating System Based on 3D Cell Culture
JIANG Zuomin,ZOU Jincheng,ZHANG AiliMed-X Research Institute, Shanghai Jiao Tong University, Shanghai 200240, China
Online:2018-03-01Published:2018-03-01摘要/Abstract
摘要： 针对目前热物理治疗的体外实验手段无法模拟动物体内的复杂环境而影响实验准确性的问题，设计并制作了一种可以基于三维培养下的细胞进行热物理实验的加热实验平台.该平台可以同时针对贴壁细胞及三维细胞进行细胞加热实验，且在加热过程中能够实时对细胞的生长状态进行观察.实验结果表明,该加热培养系统可以保持温度均匀、控制精确，同时适用于三维水凝胶进行细胞加热实验，是一种有效的三维细胞加热培养手段.
关键词: 氧化铟锡导电玻璃, 热物理治疗, 海藻酸钠, 三维培养
Abstract: To deal with the limitation of cell thermal experiment, which cannot simulate the complex micro-environment in vivo and may influence the accuracy of the experiment, the present study tried to design a petri dish which is able to heat cells both in 2D and 3D forms. At the same time, operators could constantly monitor the changes of cells during and after heating process through the microscope. Verified by experiments, this petri dish has the superiority of biocompatibility, visuality, accuracy and stability. While the 3D cell culture is used to simulate the micro-environment of the tumor in vivo, this is an effective method for cell heating and culturing.
Key words: indium tin oxides (ITO) conductive glass, thermal therapy, alginate, 3D cell culture
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