摘要/Abstract

摘要： 卵母细胞激活不足是导致卵胞浆内单精子显微注射技术失败的常见原因，其发生通常与精子无法产生足量的磷脂酶C-ζ引起钙离子（Ca²⁺）振荡来激活卵母细胞有关。为解决卵母细胞激活不足、提高卵胞浆内单精子显微注射技术成功率等，人工卵母细胞激活的相关研究及临床应用越来越多。由于其生物安全性尚未被完全确定，阐明Ca²⁺在卵母细胞激活中的作用，总结不同激活剂的原理及其临床应用现状，探讨人工卵母细胞激活技术的使用安全性及应用前景是很有必要的。
关键词: 人工卵母细胞激活, 卵母细胞激活不足, 卵胞浆内单精子显微注射, Ca²⁺振荡
Abstract:
Oocyte activation deficiency (OAD) is a common cause of failure of intracytoplasmic sperm microinjection (ICSI). The occurrence of OAD is usually related to the inability of sperm to produce sufficient amount of phospholipase C-ζ (PLCζ), causing calcium (Ca^{2 +}) oscillation to activate oocytes. In order to solve OAD and improve the success rate of ICSI, the research and clinical application of artificial oocyte activation (AOA) are becoming more and more extensive. However, its biological safety has not been fully determined. It is necessary to clarify the role of Ca^{2 +} in oocyte activation, summarize the principles of different activators and their current status in clinical application, and explore the safety and application prospect of AOA technology.
Key words: artificial oocyte activation (AOA), oocyte activation deficiency (OAD), intracytoplasmic sperm microinjection (ICSI), calcium oscillation


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