摘要： 目的·研究骨质疏松症发生及发展过程中miR-877-3p对骨髓间充质干细胞（bone mesenchymal stem cells，BMSCs）增殖能力的影响。方法·8周龄的雌性健康小鼠20只，进行双侧卵巢摘除术，建立绝经后骨质疏松模型（OVX组）。另选20只雌性健康小鼠，切除卵巢附近脂肪组织，建立假手术模型（假手术组）。术后2个月，对2组小鼠骨参数指标包括骨体积分数、骨小梁数量及骨密度行micro-CT检测；采用CCK-8法和细胞计数法检测2组BMSCs增殖能力；通过实时荧光PCR（real-time PCR）检测2组细胞miR-877-3p的表达。上调（下调）细胞内miR-877-3p水平，通过CCK-8法和细胞计数法检测BMSCs增殖能力的变化。结果·Micro-CT检测结果显示，假手术组与OVX组各项骨参数指标比较，差异均有统计学意义（
P<0.05）。CCK-8法及细胞计数结果显示，假手术组BMSCs的增殖能力明显高于OVX组（
P<0.05）。Real-time PCR结果显示，假手术组BMSCs中miR-877-3p的表达明显低于OVX组（
P<0.05）。上调（下调）miR-877-3p后，BMSCs增殖能力下降（上升）。结论·绝经后骨质疏松症小鼠中，BMSCs的增殖能力受到miR-877-3p调控，这可能是影响骨质疏松发病的重要因素之一。
关键词: 骨髓间充质干细胞, 增殖, miR-877-3p, 绝经 Abstract: Objective·To investigate the effect of miR-877-3p on the proliferation of bone marrow mesenchymal stem cells (BMSCs) during the occurrence and development of osteoporosis.
Methods·Twenty female healthy mice aged 8 weeks underwent bilateral ovariectomy to establish the postmenopausal osteoporosis models (OVX group). In addition, 20 healthy female mice were selected in the same period and the adipose tissue near the ovary was removed to establish sham operation models (Sham group). Two months after surgery, bone parameters of the mice, including bone volume fraction (BVF), trabecular number (Tb.N), and bone mineral density (BMD) were detected by micro-CT, and cell proliferation ability of the two groups was detected by CCK-8 and cell counting. The expression of miR-877-3p in the two groups was detected by real-time PCR. After up-regulating (down-regulating) the level of miR-877-3p in the BMSCs, cell proliferation was detected by CCK-8 and cell counting.
Results·The micro-CT results showed that there were significant differences in bone parameters between the Sham group and the OVX group (P<0.05). The results of CCK-8 and cell counting showed that the proliferation ability of BMSCs in the Sham group was significantly higher than that in the OVX group (P<0.05). Real-time PCR results showed that the expression of miR-877-3p in BMSCs in the Sham group was significantly lower than that in the OVX group (P<0.05). After upregulation (downregulation) of miR-877-3p, the proliferation ability of BMSCs decreased (increased).
Conclusion·In postmenopausal osteoporosis mice, the proliferation ability of BMSCs is regulated by miR-877-3p, which may be one of the important factors affecting the incidence of osteoporosis.
Key words: bone mesenchymal stem cells, proliferation, miR-877-3p, menopause PDF全文下载地址:
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