摘要： 目的·基于单细胞RNA测序（single-cell RNA sequencing，scRNA-Seq），分析严重急性呼吸综合征冠状病毒2（severe acute respiratory syndrome coronavirus 2，SARS-CoV-2）受体血管紧张素转化酶2（angiotensin-converting enzyme 2，
ACE2）在人和小鼠睾丸中的时空表达特征。方法·收集生理性各发育阶段的人睾丸组织10例和C57BL/6小鼠睾丸组织9例，用酶消化成单细胞悬液后经scRNA-Seq标准处理程序得到细胞-基因表达矩阵。经过质量控制、数据标准化、批次效应处理和聚类降维后，利用已知的睾丸细胞标志物注释各群细胞，以明确在发育的不同阶段中人和小鼠睾丸中
ACE2表达模式和差异。结果·鉴定出人和小鼠均有的睾丸细胞亚群9个，包括3群生殖细胞（精原细胞、精母细胞和精子细胞/精子）和6群体细胞（Sertoli细胞、巨噬细胞、血管平滑肌细胞、内皮细胞、Leydig细胞和管周肌样细胞）。从空间分布来看，在成人睾丸中，
ACE2主要表达于Sertoli细胞，在Leydig细胞、管周肌样细胞和生殖细胞也有表达。从时间尺度上看，人Sertoli细胞的
ACE2转录丰度随着睾丸的发育而增加，青春期后Sertoli细胞中
ACE2表达显著高于幼儿期和儿童期（
P=0.000）。从小鼠睾丸发育各阶段来看，
Ace2表达模式与人体均有显著不同。在5周龄成年C57BL/6小鼠睾丸中，
Ace2的转录水平低且主要表达于血管平滑肌细胞（
P=0.000），而Sertoli细胞中
Ace2阳性细胞数则极少。结论·SARS-CoV-2可能主要通过Sertoli细胞感染人类睾丸，常规C57BL/6小鼠模型不适用于模拟SARS-CoV-2感染对人类睾丸的功能影响。
关键词: 严重急性呼吸综合征冠状病毒2, 单细胞RNA测序, 血管紧张素转化酶2, 病毒性睾丸炎, sertoli细胞 Abstract: Objective·To analyze the spatio-temporal expression profile of angiotensin-converting enzyme 2 (ACE2), the receptor of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in human and mouse testes based on single-cell RNA sequencing (scRNA-Seq).
Methods·Ten testicular tissues from humans and nine testicular tissues from C57BL/6 mice with normal developmental stages were collected and digested into single cell suspensions by enzyme, and then the cell-gene expression matrixes were obtained by scRNA-Seq standard processing procedures. After quality control, data standardization, batch effect processing, clustering, and dimensionality reduction, each subgroup of cells was annotated based on known testicular cell bio-markers to clarify the expression patterns and differences of ACE2 in human and mouse testes with normal developmental stages.
Results·In this study, nine testicular cell subgroups found in human and mice were identified, including three subgroups of germ cells (spermatogonia, spermatocytes, and spermatids/sperm) and six subgroups of somatic cells (Sertoli cells, macrophages, vascular smooth muscle cells, endothelial cells, Leydig cells, and peritubular myoid cells). In terms of spatial distribution, ACE2 was predominantly expressed in human Sertoli cells in adult testes, and also expressed in Leydig cells, peritubular myoid cells, and germ cells. In terms of time scale, the transcription abundance of ACE2 in human Sertoli cells increased with the development of testis, and the expression level of ACE2 in Sertoli cells after puberty was significantly higher than that in infancy and childhood (P=0.000). Judging from the stages of mouse testicular development, the expression patterns of Ace2 were both significantly different from those in humans. In testis of the 5-week-old adult C57BL/6 mouse, the transcription level of Ace2 was low and it was mainly expressed in vascular smooth muscle cells (P=0.000), while the number of Ace2 positive cell in Sertoli cells was extremely low.
Conclusion·SARS-CoV-2 may mainly infects human testis through Sertoli cells, and the conventional C57BL/6 mouse model is not suitable to simulate the effect of SARS-CoV-2 infection on human testicular function.
Key words: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), single-cell RNA sequencing (scRNA-Seq), angiotensin-converting enzyme 2 (ACE2), viral orchitis, Sertoli cell PDF全文下载地址:
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