摘要： 目的·通过分析结直肠癌组织中环状RNA（circular RNA，circRNA）的差异表达谱，揭示结直肠癌的发生机制及差异表达circRNA与结直肠癌患者预后之间的关系。方法·收集上海交通大学附属第一人民医院普外科行结直肠癌根治术12例患者的肿瘤与癌旁正常组织，使用高通量测序技术检测结直肠癌及癌旁组织中circRNA的表达情况。通过基因本体（gene ontology，GO）注释分析及京都基因和基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析，对差异表达的circRNA进行功能预测。在显著表达上调与下调的circRNA中各挑选2个circRNA，采用实时荧光定量PCR（real-time fluorescent quantitative PCR，RT-qPCR）技术在44对结直肠癌组织中验证测序结果的准确性，通过搜索公共数据库构建这4种circRNA与微RNA（microRNA，miRNA）特异性结合网络图。最后结合患者预后资料判断差异表达circRNA与预后的关系。结果·经高通量测序共检测出20 924个circRNA。与癌旁正常组织比较在结直肠癌组织中异常显著表达的circRNA共有373个（︱log
2FC︱>1，
P<0.05），其中显著上调的有243个，显著下调的有130个。在44对结直肠癌样本中，RT-qPCR验证结果与高通量测序数据筛选的circRNA表达趋势基本一致。通过绘制circRNA-miRNA-mRNA互作网络后发现，与circRNA相关的miRNA与mRNA在结直肠癌患病过程中可能起着关键作用。结合患者预后信息，hsa_circ_0023984、hsa_circ_0008192高表达的患者预后较差；hsa_circ_0020093、 hsa_circ_0069922高表达的患者预后较好。结论·结直肠癌组织中存在异常表达的circRNA，这对进一步研究circRNA在结直肠癌发生和发展中的作用机制奠定了基础，对未来新型临床诊断标志物及治疗的发展提供有价值信息。
关键词: 结直肠癌, 环状RNA, 预后, 调控网络 Abstract: Objective·To reveal the mechanism of colorectal cancer and the association between differential expression of circular RNAs (circRNAs) and patients' prognosis.
Methods·Colorectal cancer tissues and normal tumor-adjacent tissues were harvested from 12 patients undergoing radical resection of colorectal cancer in the Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, and the expressions of circRNAs were detected by high-throughput sequencing. Function predictions of differentially expressed circRNAs were carried out by gene ontology (GO) annotation analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Two circRNAs were selected from the significantly up-regulated and down-regulated circRNAs respectively, and the accuracy of sequencing results was verified in 44 sets of colorectal cancer tissues by real-time fluorescent quantitative PCR (RT-qPCR). The circRNA-miRNA targeting binding network map was constructed by searching the public database for the four circRNAs. Finally, the relationships between differential expression of circRNAs and prognosis were determined based on the patients' clinical data.
Results·A total of 20 924 differentially expressed circRNAs were detected after high-throughput sequencing. Compared with normal colorectal tissues, there were 373 circRNAs (︱log2FC︱>1, P<0.05) abnormally and significantly expressed in colorectal cancer tissues, of which 243 were significantly up-regulated and 130 were significantly down-regulated. In 44 pairs of colorectal cancer samples, the results verified by RT-qPCR were basically consistent with the sequencing data. After the circRNA-miRNA-mRNA interactions being plotted, it was found that miRNA and target genes associated with circRNA may play an important role in the pathogenesis of colorectal cancer. Combined with the prognosis information, the patients with high expression of hsa_circ_0023984 or hsa_circ_0008192 had poorer prognosis while patients with high expression of hsa_circ_0020093 or hsa_circ_0069922 had better prognosis.
Conclusion·Abnormally expressed circRNAs in colorectal cancer tissues lay a foundation for further studies on the occurrence and development of colorectal cancer, and provide valuable information for the development of new clinical diagnostic markers and treatments in the future.
Key words: colorectal cancer, circular RNA (circRNA), prognosis, regulatory network PDF全文下载地址:
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