摘要/Abstract
摘要： 目的·分析子宫内膜癌组织和正常内膜组织中长链非编码RNA（long noncoding RNA，lncRNA）的差异表达谱。方法·分别抽提21例子宫内膜癌组织和5例正常内膜组织的RNA，利用转录组测序技术分析并筛选2组差异表达lncRNAs。对获得的差异表达lncRNAs进行GO（Gene Ontology）功能分析和KEGG（Kyoto Encyclopedia of Genes and Genomes）通路分析，并结合TCGA数据库对差异表达lncRNAs进行交集分析。结果·共筛选获得3 060个差异表达lncRNAs，其中上调表达的有2 046个，下调的有1 014个。GO功能分析和KEGG通路分析结果显示，这些lncRNAs与细胞黏附、免疫反应、炎症反应、细胞增殖相关，并且主要富集在PI3K-Akt信号通路、细胞黏附、细胞因子受体等通路上。交集分析显示，57个lncRNAs在测序结果和TCGA数据库中同时上调或下调。结论·lncRNAs在子宫内膜癌和正常内膜组织中的表达有显著差异，提示其可能在子宫内膜癌的发生及发展中起到了重要作用。
关键词: 子宫内膜癌, 长链非编码RNA, 基因表达谱
Abstract:
Objective · To analyze the differentially expressed profiles of long noncoding RNA (lncRNA) in endometrial cancer (EC) tissues and normal endometrial tissues. Methods · The RNA was extracted from 21 EC tissues and 5 normal endometrial tissues, respectively, and lncRNAs expression profiles were analyzed and screened by transcriptome sequencing technology. Gene Ontology (GO) function analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were carried out for the differentially expressed lncRNAs, and their expression differences between the transcriptome sequencing and TCGA database were analyzed. Results · There were 3 060 differentially expressed lncRNAs, of which 2 046 were up-regulated and 1 014 were down-regulated. GO functional analysis showed that these lncRNAs were associated with cell adhesion, immune response, inflammatory response and cell proliferation. KEGG pathway analysis showed that these lncRNAs were mainly enriched on the pathways, such as PI3K-Akt signaling pathway, cell adhesion and cytokine-cytokine receptor interaction. Intersection analysis showed that 57 lncRNAs were up-regulated or down-regulated simultaneously in the sequencing results and TCGA database. Conclusion · The expression of lncRNAs in EC tissues and normal endometrial tissues are significantly different, suggesting that it may play an important role in the occurrence and development of EC.
Key words: endometrial cancer (EC), long noncoding RNA (lncRNA), gene expression profile
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