摘要/Abstract
摘要： 目的·探究不同类型小鼠胎肝基质细胞在细胞形态、分子特征和生物学功能上的差异。方法·通过体外贴壁培养的方式获取13.5 d小鼠胎肝基质细胞，根据细胞形态及其表面标志物CD44、CD29、CD106、CD45及Sca-1的表达差异，区分不同的细胞类型。使用小鼠基因组阵列430 2.0基因芯片在P3或-2的标准下筛选差异基因，并使用IPA（ingenuity pathway analysis）软件对典型信号通路和生物学功能进行分析。结果·依据细胞形态的不同，分离出2类胎肝基质细胞，即短梭形细胞（CD45+CD106-CD29+CD44+Sca-1-）和成纤维样细胞（CD45-CD106+CD29+CD44+Sca-1-）。前者共筛选出1 485个高表达基因，主要参与免疫炎症相关的信号通路；后者共筛选出3 374个高表达基因，主要参与细胞外基质形成及细胞间黏附。结论·小鼠胎肝基质细胞在生物学特征及功能上具有较强的异质性，在促进造血干细胞的增殖方面可能存在差异。
关键词: 胎肝, 基质细胞, 造血干细胞, 流式细胞术, 基因芯片, IPA软件
Abstract:
Objective · To explore the differences in morphology, molecular characteristics and biological functions of different types of mofetal liver stromal cells. Methods · E13.5 mofetal liver stromal cells were obtainedadherent culture, and different cell types were distinguishedmorphology and of surface markers, such as CD44, CD29, CD106, CD45 and Sca-1. Then microarray assay was conductedMoGenome 430 2.0 Array and analyzed at P3 or -2 to identify differential genes. Canonical pathway and biological function analyses were performedusing ingenuity pathway analysis (IPA software). Results · Spindle-like (CD45+CD106-CD29+CD44+Sca-1-) and fibroblast-like (CD45-CD106+CD29+CD44+Sca-1-) fetal liver stromal cells were isolated in this study according to the cell morphology. The 1 485 highly-expressed genes in spindle-like cells mainly involved in immune and inflammation-related signaling pathways; while the 3 374 highly-expressed genes in fibroblast-like cells mainly involved in extracellular matrix formation and cellular adhesion. Conclusion · Mofetal liver stromal cells have strong heterogeneity in biological characteristics and functions, especially in hematopoietic promoting capability.
Key words: fetal liver, stromal cell, hematopoietic stem cell (HSC), flow cytometry, microarray, ingenuity pathway analysis (IPA)
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