摘要/Abstract

摘要： 目的 ·分析 Fbxo22基因敲除小鼠的表型，为探索 FBXO22的生物学功能提供理论依据。方法 ·利用 CRISPR-Cas9（clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9）技术成功构建 Fbxo22全身敲除小鼠，观察胚胎和小鼠的外观，测定其数量和质量，并分析小鼠的进食量和存活时间。结果 · Fbxo22敲除小鼠胚胎期 17.5/18.5 d的胚胎数量符合孟德尔遗传定律，外观未见异常，但是多数 Fbxo22敲除小鼠在出生后 48 h内死亡。少数存活小鼠体型偏小，进食减少，存活时间缩短。结论 · FBXO22对于小鼠出生后早期存活和正常发育有重要作用。
关键词: FBXO22, CRISPR-Cas9技术, 早期生后死亡
Abstract:
Objective · To establish the Fbxo22 knockout momodel and study the biological function of FBXO22. Methods · The Fbxo22 knockout mice were generatedCRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/-mice were analyzed. Results · Although the Fbxo22-/-embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/- mice showed reduced body size and food intake and decreased life span. Conclusion · FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development. [Key words]FBXO22; CRISPR-Cas9 technology; early postnatal lethality
Key words: FBXO22, CRISPR-Cas9 technology, early postnatal lethality


PDF全文下载地址:

点我下载PDF