摘要/Abstract
摘要： 目的 ·研究选择性 α1肾上腺素能受体激动剂去氧肾上腺素（ phenylephrine, Phe）及拮抗剂哌唑嗪（ prazosin，Pra）对阿霉素 (doxorubicin，DOX)诱导心肌细胞凋亡的影响。方法 ·将大鼠 H9C2心肌细胞分为 4组；除对照组外，其他 3组均加入 1.8 μmol/L DOX；DOX组仅给予 DOX，激动剂组同时加入 0.1 mmol/L Phe，拮抗剂组同时加入 10 μmol/L Pra。细胞培养 24 h后，采用流式细胞术及 TUNEL染色检测细胞凋亡；Western blotting检测凋亡效应物 cleaved caspase 3的表达；实时定量 PCR检测 Bcl-2家族抗凋亡基因及促凋亡基因在 mRNA水平的表达； CCK-8检测细胞活力。结果 · Phe抑制 DOX诱导的心肌细胞凋亡， cleaved caspase 3表达量降低； Pra促进 DOX诱导的心肌细胞凋亡， cleaved caspase 3表达量升高。与 DOX组比较，激动剂组 Bcl-2、Bcl-w在 mRNA水平的表达升高， Bax、Bad 在 mRNA水平的表达降低；而拮抗剂组 Bcl-2、Bcl-w的表达降低， Bax、Bad的表达升高。给药 24 h后，激动剂组心肌细胞活力较 DOX组增高，拮抗剂组细胞活力与 DOX组无明显差异。结论 · DOX诱导的心肌细胞凋亡受 α1肾上腺素能信号通路调节。
关键词: 心肌细胞, 阿霉素, 凋亡, 去氧肾上腺素, 哌唑嗪
Abstract:
Objective · To investigate the effects of α1-adrenergic receptor agonist phenylephrine (Phe) and antagonist prazosin (Pra) on cardiomyocyte apoptosis induceddoxorubicin (DOX). Methods · H9C2 cardiomyocytes were divided into 4 groups. Except for the control group incubated with medium alone, all other groups were treated with 1.8 μmol/L DOX. For agonist group and antagonist group, 0.1 mmol/L Phe and 10 μmol/L Pra were added respectively in DOX-treated cells. After culture for 24 h, flow cytometry and TUNEL assay were performed to detect the apoptosis rate. Western blotting was used to detect the of cleaved caspase 3. Real-time PCR was used to detect the of anti- and pro-apoptotic Bcl-2 family genes. CCK-8 assay was used to detect the cell viability. Results · The DOX-induced apoptosis was inhibitedPhe with decreased apoptosis rate of H9C2 and decreased of cleaved caspase 3, but promotedPra. Increased of Bcl-2 and Bcl-w and decreased of Bax and Bad at mRNA levels were found in agonist group in comparison with the cells treated with DOX alone; while decreased of Bcl-2 and Bcl-w and increased of Bax and Bad were found in antagonist group. The cell viability after 24 h of treatment with agonist was higher than cells treated with DOX alone, but no signifiant difference was found in cell viability between antagonist group and DOX group. Conclusion · α1-Adrenergic signaling pathway may be involved in endogenous myocardial protection in the process of cardiomyocyte apoptosis inducedDOX.
Key words: cardiomyocyte, doxorubicin, apoptosis, phenylephrine, prazosin
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