摘要/Abstract
摘要： 目的 ·探讨孕前和孕期维生素 D缺乏对子代大鼠肠道菌群及抗菌肽 cathelicidin（cathelicidin antimicrobial peptide，CAMP）的影响。方法 ·将 24只 8周龄雌性 SD大鼠分为 3组，对照组（ C组）、维生素 D缺乏组（ VDD组）及维生素 D补充组（ VDS组）各 8只。通过特殊饲料饮食构建大鼠孕前和孕期维生素 D缺乏模型。母鼠孕第 14日用液相色谱 -串联质谱联用法检测血清 25(OH)D水平。子鼠 4周龄时，取血检测 25(OH)D水平；取粪便检测肠道菌群；取结肠组织，用实时荧光定量 RT-qPCR法、Western blotting法分别测定大鼠 CAMP的 mRNA及蛋白表达水平。结果 ·大鼠孕前和孕期维生素 D缺乏模型构建成功。 C组、VDD组及 VDS组子鼠肠道乳酸杆菌的相对丰度分别为 0.050±0.016、0.028±0.013及 0.033±0.021。与 C组相比， VDD组子鼠肠道乳酸杆菌的相对丰度显著降低（ P<0.05），结肠 CAMP的 mRNA及蛋白表达水平亦显著降低（均 P<0.05）。与 VDD组相比， VDS组子鼠肠道乳酸杆菌的相对丰度有升高的趋势，但差异无统计学意义；结肠 CAMP的 mRNA表达水平无明显改变，蛋白表达水平显著升高（ P<0.05）。结论 ·孕期维生素 D缺乏可致子代大鼠结肠 CAMP的 mRNA及蛋白表达水平和肠道乳酸杆菌的相对丰度显著降低。孕期维生素 D补充后，子代大鼠结肠 CAMP的蛋白表达水平显著升高，肠道乳酸杆菌的丰度也有升高的趋势。
关键词: 孕期, 维生素 D, 肠道菌群, 抗菌肽 cathelicidin
Abstract:
Objective · To explore the effects of maternal vitamin D deficiency before and during pregnancy on the intestinal flora and cathelicidin antimicrobial peptide (CAMP) in offspring rats. Methods · Twenty-four female Sprague-Dawley rats (8-week-old) were randomly assigned to three groups (n8 per group), i.e. control group (C group), vitamin D deficiency group (VDD group), and vitamin D supplement group (VDS group). Special diets were used to build the rat models of vitamin D deficiency before and during pregnancy. Maternal 25(OH)D level was detectedliquid chromatography tandem mass spectrometry (LC-MS/MS) at gestational day 14.At the age of 4 weeks in offsprings, the level of 25(OH)D was measured; the faeces were collected for the detection of intestinal flora; the mRNA and protein of CAMP were evaluatedreal-time quantitative RTPCR and Western blotting, respectively. Results · The rat models of vitamin D deficiency before and during pregnancy were successfully established.The relative abundance of intestinal Lactobacillus in theoffspring rats in C group,VDD group andVDS group was 0.050±0.016,0.028±0.013 and0.033±0.021, respectively. Compared with C group, the relative abundance of intestinal Lactobacillus in VDD group significantly decreased (P<0.05)，and the mRNA and protein of colonic CAMPsignificantly decreased as well (P<0.05). Compared with VDD group, VDS group showed a trend of increase in the abundance of intestinal Lactobacillus;themRNA ofcolonic CAMPdidn’tchange significantly, but the proteinof colonic CAMP significantly increased (P<0.05). Conclusion · Vitamin D deficiency during pregnancy results in decreased abundance of intestinal Lactobacilli as well as reduced mRNAand protein of colonic CAMPin offspring rats. Vitamin D supplementation significantly improves the protein of colonic CAMP and induces a trend of increase in the abundance of intestinal Lactobacilli in offspring rats.
Key words: pregnancy, vitamin D, intestinal flora, cathelicidin antimicrobial peptide(CAMP)
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