摘要/Abstract
摘要： 目的 · 建立稳定的大鼠体外肺灌注（ex vivo lung perfusion, EVLP）模型，并且与传统的冷保存方法进行保存效果的对比。
方法 · 对 8 只 SD 大鼠进行 EVLP 转流 4 h，对另外 8 只进行传统冷保存 3 h+ EVLP 1h。比较 2 组肺生理指标、湿干比、伊文思蓝染
色浓度的差异。结果 · EVLP 组的肺湿干比值显著低于传统冷保存组（5.08±0.88 vs 6.09±0.48, P=0.012），氧合指数显著高于传统冷
保存组 [（337.9±35.5）mmHg vs（300.5±21.6）mmHg，P=0.023]，伊文思蓝浓度显著低于传统冷保存组 [（36.5±20.3）μg/mL vs
（65.0±29.9）μg/mL，P=0.043]。结论 · EVLP 作为传统冷保存法的一种有效替代方法，能够较好地延缓肺部损伤。
关键词: &ensp, 体外肺灌注；冷保存；肺移植；大鼠
Abstract:
Objective · To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold
storage group. Methods · Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP. The biological
index, wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups. Results · Wet-to-dry weight ratio of lung tissue in
EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48, P=0.012); Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg
vs (300.5±21.6) mmHg, P=0.023]; Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL,
P=0.043]. Conclusion · EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.
Key words: ex vivo lung perfusion, cold storage, lung transplantation, rat
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