摘要/Abstract
摘要： 目的 · 结合对氧磷酶 1（paraoxonase 1，PON1）基因型探讨孕妇有机磷农药（organophosphate pesticides，OPs）暴露水平与体内
氧化应激水平之间的关系。方法 · 以山东省某医院的 204 名孕晚期妇女为研究对象，检测尿液中 4 种 OPs 非特异性二烷基磷酸酯代谢
产物水平，测定血清中的氧化应激标志物 [ 总自由巯基（-SH）、丙二醛（MDA）] 水平，并提取全血中的 DNA 检测 PON1-108、PON1192、
PON155 位点的基因型。采用多元线性回归模型分析全人群及不同 PON1 基因型的个体中 OPs 暴露水平与氧化应激标志物水平之间的关
联。结果 · 未见 OPs 暴露与氧化应激之间存在显著关联。但根据 PON1 基因型分层后发现在 PON1192RR 基因型的孕妇中，总磷酸二甲酯类
水平 [β（95% CI）：-104.10（-191.31，-16.88）] 及总二烷基磷酸酯类水平 [β（95% CI）：-111.78（-221.84，-1.72）] 与 -SH 水平呈负相
关，而在其他基因型的个体中未发现这一关联。结论 · OPs 暴露可能与 PON1192RR 基因型的孕妇体内氧化应激水平升高有关。
关键词: 氧化应激, 有机磷农药, 对氧磷酶 1, 孕妇
Abstract:
To investigate whether paraoxonase 1 (PON1) genotypes were effect modifiers in the relationship between exposure to organophosphate pesticides (OPs) and oxidative stress level in pregnant women. Methods · A total of 204 pregnant women recruited from a hospital in Shandong Province were included in the study. Four nonspecific dialkyl phosphate (DAP) metabolites of OPs were measured in each urine sample. Levels of two oxidative stress biomarkers [total free sulfhydryl (-SH) and malondialdehyde (MDA)] were measured in serum samples. Blood samples were also analyzed for detecting PON1 genotypes (PON1-108, PON1192 and PON155). Separate linear regression models were conducted to explore the relationship between DAP metabolite levels and oxidative stress levels in all 204 pregnant women or women within each PON1 genotype. Results · There was no significant association between DAP metabolite levels and oxidative stress levels in all 204 women. Levels of dimethyl phosphates [β (95% CI): -104.10 (-191.31, -16.88)] and dialkyl phosphates [β (95% CI): -111.78 (-221.84, -1.72)] were negatively associated with -SH level among pregnant women with PON1192RR genotype, but this association was not found among women with other genotypes. Conclusion · OP exposure may be associated with a higher oxidative stress level among pregnant women with PON1192RR genotype.
Key words: oxidative stress, organophosphate pesticides, paraoxonase 1, pregnant women
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