导师介绍

导师姓名
刘喜朋
导师性别男
职务职称副教授.
所在院系生命科学技术学院
所属学科生物学
研究方向DNA损伤修复耐热蛋白质基因工程
联系电话021-34204192
电子邮箱xpliu@sjtu.edu.cn

个人简介

刘喜朋，博士、副教授。1994－2001就读于天津大学，获工学学士、工学硕士。2001-2005就读上海交通大学，获博士学位。2005年至今在上海交大生命科学技术学院任教，主要从事DNA复制、损伤与修复，核酸检测与SNP分型新技术研发，嗜热微生物蛋白在生命科学中的应用等研究。研究结果主要包括：对衣原体DNA碱基切除修复进行了比较系统的研究，克隆表达了多个参与衣原体碱基切除修复的蛋白质，体外鉴定了这些蛋白质的酶学性质和生物学功能。克隆了嗜热微生物的近500个DNA复制与损伤修复基因，对部分蛋白进行了功能研究。现已发表20篇SCI论文，2片核心期刊论文；申请了5项关于核酸检测、SNP测定、基因克隆的国家发明专利，其中3项已经获得授权。
代表性论著

1.LiuXipeng,ZhangYang,LiangRubing,HouJingli,LiuJianhua.Characterizationofthe3'exonucleaseofChlamydophilapneumoniaeendonucleaseIVondouble-strandedDNAandtheRNAstrandofRNA/DNAhybrid.BiochemBiophysResCommun.2007Oct5;361(4):987-993.(IF:2.8)2.LiuXipeng,LiuJianhua.ThemechanismofbaseexcisionrepairinChlamydiophilapneumoniae.DNARepair.2005Nov21;4(11):1295-1305.(IF:5.9)3.LiuXipeng,LiuJianhua.ChlamydiapneumoniaeAPendonucleaseIVcouldcleaveAPsitesofdouble-andsingle-strandedDNA.BiochimBiophysActa.2005Dec1;1753(2):217-225.(IF:3.3)4.LiuXipeng,HouJingli,LiuJianhua.ChlamydialDNApolymeraseIcanbypasslesionsinvitro.BiochemBiophysResCommun.2006Jul7;345(3):1083-1091.(IF:2.8)5.LiuXipeng,LiuJianhua.Signalpeptidedoesnotinhibitbindingofbiotintostreptavidin.BiotechnolLett.2005Aug;27(15):1067-1073.(IF:1.7)6.LiuXipeng,LiuJianhua.Cloning,expression,andcharacterizationofuracil-DNAglycosylaseofChlamydiapneumoniaeinEscherichiacoli.ProteinExprPurif.2004May;35(1):46-53.(IF1.9)7.LiuXP,LiuJH.Theterminal5'phosphateandproximatephosphorothioatepromoteligation-independentcloning.ProteinSci.2010May;19(5):967-73.(IF3.3)8.LiuXP,HouJL,LiuJH.Anovelsinglenucleotidepolymorphismdetectionofadouble-strandedDNAtargetbyaribonucleotide-carryingmolecularbeaconandthermostableRNaseHII.AnalBiochem.2010Mar1;398(1):83-92.(IF3.3)9.LiuXP,LiCP,HouJL,LiuYF,LiangRB,LiuJH.Expressionandcharacterizationofthymine-DNAglycosylasefromAeropyrumpernix.ProteinExprPurif.2010Mar;70(1):1-6.(IF1.9)10.PeiDongli,LiuJianhua,LiuXipeng,LiSuolian.ExpressionofbothChlamydiapneumoniaeRNaseHIIsinEscherichiacoli.ProteinExprPurif.2005Mar;40(1):101-106.(IF1.9)11.ZhangYi,LiuXipeng,Liu,Jianhua.RecombinanthumanalphaA-crystallincanprotecttheenzymaticactivityofCpUDGagainstthermalinactivation.FEBSLett.2005May23;579(13):2897-2900.(IF3.4)12.LiangRubing,LiuXipeng,LiuJianhua,RenQiushi,LiangPeiji,LinZhixin,XieXiangming.AT7-expressionsystemundertemperaturecontrolcouldcreatetemperature-sensitivephenotypeoftargetgeneinEscherichiacoli.JMicrobiolMethods.2007Mar;68(3):497-506.(IF:2.4)13.LiangRubing,LiuXipeng,PeiDongli,LiuJianhua.BiochemicalcharacterizationandfunctionalcomplementationofribonucleaseHIIandribonucleaseHIIIfromChlamydophilapneumoniaeAR39.Microbiology（UK）.2007Mar;153(Pt3):787-793.(IF:3.1)14.HouJingli,LiuXipeng,PeiDongli,LiuJianhua.RNaseHIIfromChlamydiapneumoniaediscriminatesmismatchesincorporationintoDNA-rN1-DNA/DNAduplexes.BiochemBiophysResCommun.2007May18;356(4):988-992.(IF2.8)15.HouJingli,LiuXipeng,ZhengYan,LiuJianhua,DuanTao.MolecularbeaconsforisothermalfluorescenceenhancementbythecleavageofRNaseHIIfromChlamydiapneumoniae.AnalBiochem.2007Dec15;371(2):162-166.(IF:3.3)16.HouJingli,LiuXipeng,ZhengYan,LiuJianhua.AnefficientcloningofDNAfragmentsbyamethodbasedonuracil-DNAglycosylaseandendonucleaseIV.JBiochemBiophysMethods.2008Apr24;70(6):1196-1198.(IF1.4)17.HouJingli,LiuXipeng,ZhengYan,LiuJianhua.AMethodforHLAGenotypingUsingtheSpecificCleavageofDNA-rN1-DNA/DNAwithRNaseHIIfromChlamydiapneumoniae.Oligonucleotides,2007Winter,17(4):433-444.(IF2.8)