摘要/Abstract

合成了一种(E)-2-(3-氰基-4-(3-甲酰基-4-羟基苯乙烯基)-5,5-二甲基呋喃-2(5H)-亚烷基)丙二腈衍生物CDM, ¹H NMR、¹³C NMR和HRMS验证了其结构. CDM在MeCN/Tris (V∶V=2∶8, pH=7.4)溶液中通过荧光信号“ON-OFF”识别HSO₃^-/SO₃^2-, 颜色由粉色变为无色. 探针CDM具有良好的抗干扰能力和较快的响应速度(200 s), pH适用范围为7~14, 荧光光谱和紫外吸收光谱的检测限分别为6.07和1.59 μmol/L. 实验研究表明, CDM能检测真实水样和糖样中的HSO ₃^-/SO₃^2-, 并能够对活细胞中的HSO₃^-/SO₃^2-进行荧光成像.
关键词: 荧光探针, HSO₃^-/SO₃^2-, 长波长, 裸眼识别, 细胞成像
3-Cyano-4-(3-formyl-4-hydroxystyryl)-5,5-dimethylfuran-2(5H)-alkylene)malononitrile derivative CDM was synthesized. The correctness of its structure was verified by ¹H NMR, ¹³C NMR and HRMS. CDM can recognize HSO₃^-/SO₃^2- with the naked eye through the fluorescent signal “ON-OFF” in MeCN/Tris ( V∶V=2∶8, pH=7.4) solution, and the color changes from pink to colorless. The probe CDM has good anti-interference ability and fast response speed (200 s), the pH applicable range is 7~14, and the detection limits of the fluorescence and UV absorption spectrum are 6.07 and 1.59 μmol/L. The experimental research shows that CDM can detect HSO₃^-/SO₃^2- in real water samples and sugar samples and, can perform fluorescent image HSO₃^-/SO₃^2- in living cells.
Key words: fluorescent probe, HSO₃^-/SO₃^2-, long wavelength, naked eye recognition, cell imaging


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