Sprague-Dawley与Wistar大鼠双侧颈总动脉结扎后大脑病理变化和认知功能的差异
孙伟, 耿悦, 陈叶婷, 唐小杭, 张永杰, 顾胜华, 谢家骏, 张泽安, 田雪松*
上海中医药大学创新中药研究院,上海 201203
摘要
本文旨在研究Sprague-Dawley (SD)与Wistar大鼠双侧颈总动脉结扎(bilateral common carotid artery occlusion, BCCAO)后的大脑病理变化及认知功能差异。雄性SD和Wistar大鼠分别随机分为2组:假手术(S-sham和W-sham)组与手术(S-BCCAO和W-BCCAO)组。BCCAO术后1、3、7、14及28 d,观察各组存活率及瞳孔对光反射(pupillary light reflex, PLR)消失率;术后28 d行明暗箱、Y迷宫及新气味识别测试,检测大鼠认知功能;用HE和Luxol fast blue染色法观察灰质(海马)、白质(视束)、视神经和视网膜的病理改变。结果显示,W-BCCAO组大鼠的存活率为62.5%,PLR全部消失;S-BCCAO组大鼠的存活率100%,PLR消失率为58.3%。和各自的假手术组相比,W-BCCAO组大鼠在明箱的滞留时间及活动距离比例均显著增加,S-BCCAO组大鼠则无明显变化;S-BCCAO组大鼠在Y迷宫III臂(迷宫臂)的滞留时间及活动距离比例均显著减少,而W-BCCAO组大鼠无明显变化;S-BCCAO组大鼠的新气味辨识比率显著降低,而W-BCCAO组大鼠无明显变化。S-BCCAO组大鼠海马CA1区可见缺血性损伤,而W-BCCAO组无明显损伤;S-BCCAO及W-BCCAO组大鼠均可见视束、视神经缺血性损伤;S-BCCAO、W-BCCAO大鼠视网膜损伤明显,总厚度明显变薄,其中S-BCCAO组大鼠可见节细胞层(ganglion cell layer, GCL)、内网层(inner plexiform layer, IPL)及外网层(outer plexiform layer, OPL)变薄,其它各层与S-sham组相比无显著差异,W-BCCAO组大鼠除外核层(outer nuclear layer, ONL)外,其余各层与W-sham组相比均变薄。上述结果表明,SD和Wistar大鼠BCCAO术后海马和视觉传导通路病理变化存在差异,学习记忆损伤程度也有所不同,提示应根据研究目的选择不同品系大鼠建立血管性痴呆模型。
关键词: 脑缺血; 血管性痴呆; 双侧颈总动脉结扎; 视网膜
分类号:R332;R363.2;R743.3
Differences of brain pathological changes and cognitive function after bilateral common carotid artery occlusion between Sprague-Dawley and Wistar rats
SUN Wei, GENG Yue, CHEN Ye-Ting, TANG Xiao-Hang, ZHANG Yong-Jie, GU Sheng-Hua, XIE Jia-Jun, ZHANG Ze-An, TIAN Xue-Song*
Innovation Research Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Abstract
The aim of the present study was to investigate the differences of the pathological changes and cognitive function after
bilateral common carotid artery occlusion (BCCAO) between Sprague-Dawley (SD) and Wistar rats. Male SD and Wistar rats were randomly divided into 2 groups, respectively: sham operated (S-sham and W-sham) and operated (S-BCCAO and W-BCCAO) groups. The survival rate and the rate of loss of pupillary light reflex (PLR) were observed on day 1, 3, 7, 14 and 28 after the operation, and the light-dark box, Y-maze and odor recognition tests were performed to detect cognitive function on day 28 after the operation. HE and Luxol fast blue staining were used to observe the pathological changes of gray matter (hippocampus), white matter (optical tract), optic nerve, and retina. The results showed that the survival rate of the W-BCCAO group was 62.5%, and PLR loss rate was 100%; whereas the survival rate of the S-BCCAO group was 100%, and PLR loss rate was 58.3%. In the W-BCCAO group, percentages of time spent and distance traveled in the light box of were more than those in the W-sham group, but there was no statistical significance between the S-BCCAO and S-sham groups. In the S-BCCAO group, the percentages of time spent and distance traveled in the III arm (labyrinth arm) of the Y-maze were less than those in the S-sham group, but no statistical significance was found between the W-BCCAO group and W-sham group. In the S-BCCAO group, the discrimination ratio of the odor recognition task was less than that in the S-sham group, but no statistical significance could be seen between the W-BCCAO and W-sham groups. Ischemic injury was observed in the CA1 area of the hippocampus in the S-BCCAO group, but no readily visible damage was observed in the W-BCCAO group. Ischemic injury of the visual beam and optic nerve was observed in both the S-BCCAO and W-BCCAO groups. Compared with the corresponding sham groups, the S-BCCAO and W-BCCAO groups showed serious retinal damage with significant thinner retina. The ganglion cell layer (GCL), inner plexiform layer (IPL), and outer plexiform layer (OPL) were thinner in the S-BCCAO group, but no statistical significances were shown in the other layers. All the layers, except the outer nuclear layer (ONL), were significantly thinner in the W-BCCAO group. The results indicate that there are differences of the pathological changes in the hippocampus and visual conduction pathway after BCCAO between SD and Wistar rats, and the degree of learning and memory injury was also different, which suggests that the vascular dementia model of different rat strains should be selected according to research purpose.
Key words: Cerebral ischemia; vascular dementia; bilateral occlusion of the common carotid arteries; retina
收稿日期:2018-12-10 录用日期:2019-05-17
通讯作者:田雪松 E-mail: xuesong.tian@shutcm.edu.cn
DOI: 10.13294/j.aps.2019.0065
引用本文:
孙伟, 耿悦, 陈叶婷, 唐小杭, 张永杰, 顾胜华, 谢家骏, 张泽安, 田雪松. Sprague-Dawley与Wistar大鼠双侧颈总动脉结扎后大脑病理变化和认知功能的差异[J]. 生理学报 2019; 71 (5): 705-716.
SUN Wei, GENG Yue, CHEN Ye-Ting, TANG Xiao-Hang, ZHANG Yong-Jie, GU Sheng-Hua, XIE Jia-Jun, ZHANG Ze-An, TIAN Xue-Song. Differences of brain pathological changes and cognitive function after bilateral common carotid artery occlusion between Sprague-Dawley and Wistar rats. Acta Physiol Sin 2019; 71 (5): 705-716 (in Chinese with English abstract).
PDF全文下载地址:
点我下载PDF
删除或更新信息,请邮件至freekaoyan#163.com(#换成@)
Sprague-Dawley与Wistar大鼠双侧颈总动脉结扎后大脑病理变化和认知功能的差异
本站小编 Free考研考试/2022-02-13
相关话题/病理 上海 比例 大鼠 测试
白介素6通过阻断L型钙离子通道活性抑制急性胰腺炎大鼠结肠纵行肌条收缩
白介素6通过阻断L型钙离子通道活性抑制急性胰腺炎大鼠结肠纵行肌条收缩唐亚1,梁世伟1,全晓静2,罗和生2,刘颖1,*1桂林医学院第二附属医院消化科,桂林541100;2武汉大学人民医院消化科,武汉430060摘要本文旨在探讨白介素6(interleukin6,IL-6)对急性胰腺炎(acutepan ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13富氢水在黄曲霉毒素B1致大鼠肝损伤模型中的抗损伤作用
富氢水在黄曲霉毒素B1致大鼠肝损伤模型中的抗损伤作用扈红蕾1,*,高健1,郭文君2,周风华2,刘红燕1,宿长春11潍坊护理职业学院潍坊校区,潍坊261041;2潍坊医学院病理学教研室,潍坊261042摘要本文旨在研究富氢水在黄曲霉毒素B1(aflatoxinB1,AFB1)致大鼠急性肝损伤模型中的抗 ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13鞘内激活MrgC受体在病理性疼痛和吗啡耐受中的作用
鞘内激活MrgC受体在病理性疼痛和吗啡耐受中的作用李幼萍*,江剑平福建师范大学生命科学学院,福建省发育和神经生物学重点实验室,福州350117摘要啮齿类动物的MrgC受体(Mas-relatedG-protein-coupledreceptorsubtypeC)与人类Mas相关基因X受体1(huma ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13乙酰胆碱通过作用于α7烟碱型乙酰胆碱受体抑制大鼠小胶质细胞炎性反应
乙酰胆碱通过作用于α7烟碱型乙酰胆碱受体抑制大鼠小胶质细胞炎性反应蒋永莹,李霖,沈卫星,邱一华,彭聿平*南通大学医学院生理学系,南通226001摘要小胶质细胞是中枢神经系统中主要的免疫细胞。本研究旨在探讨乙酰胆碱(acetylcholine,ACh)抑制小胶质细胞炎症反应的具体机制。原代培养Spra ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13下丘脑室旁核apelin对手术创伤大鼠心功能的保护作用
下丘脑室旁核apelin对手术创伤大鼠心功能的保护作用张环环1,2,王雅静1,郑超1,汪萌芽1,*,朱大年21皖南医学院细胞电生理研究室,芜湖241002;2复旦大学基础医学院生理与病理生理学系,上海200032摘要Apelin是一种新型的内源性活性肽。本研究旨在探寻下丘脑室旁核(paraventr ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13内源性CO在大鼠离体心脏缺血再灌注中的保护作用
内源性CO在大鼠离体心脏缺血再灌注中的保护作用周振,马爽,刘杰,纪巧荣,曹成珠,李晓娜,汤锋,张伟*青海大学高原医学研究中心;青海省高原医学应用基础重点实验室,西宁810001摘要本文旨在观察研究内源性CO在大鼠离体心脏缺血再灌注中的作用。大鼠经内源性CO激动剂原卟啉氯化钴(CoPP)和内源性CO抑 ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13AMPA受体参与的出生后大鼠海马发育早期的电生理学特点
AMPA受体参与的出生后大鼠海马发育早期的电生理学特点陈雪怡1,张皑峰2,赵文1,3,4,高钰丹1,3,4,段红梅1,3,4,郝鹏1,3,4,杨朝阳1,3,4,李晓光1,3,4,*1首都医科大学神经生物学系,北京100069;2首都医科大学附属北京友谊医院口腔科,北京100050;3北京航空航天大学 ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13内侧隔核注射淀粉样β蛋白损害大鼠的长时程增强和认知行为
内侧隔核注射淀粉样β蛋白损害大鼠的长时程增强和认知行为武美娜1,孔林林1,2,张军1,胡梦明1,王昭君1,蔡红艳3,祁金顺1,*1山西医科大学生理学系,细胞生理学省部共建教育部重点实验室,太原030001;2浙江大学医学院附属第二医院神经内科,杭州310009;3山西医科大学微生物与免疫教研室,太原 ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13内皮素受体拮抗剂波生坦通过降低肾交感神经活性改善间歇性低氧大鼠的高血压
内皮素受体拮抗剂波生坦通过降低肾交感神经活性改善间歇性低氧大鼠的高血压杨胜昌1,郭亚净2,于富洋1,陈玲玲3,李文雅1,吉恩生1,*1河北中医学院生理教研室,石家庄050200;2河北中医学院科研中心,石家庄050200;3杭州市西溪医院药剂科,杭州310012摘要本文旨在观察内皮素受体拮抗剂波生坦 ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13SOS1蛋白病理生理功能研究进展
SOS1蛋白病理生理功能研究进展郝佩琪1,张新宇1,郭慧1,2,杨洋1,安输1,刘莹1,郭晓汐1,徐天瑞1,郝倩1,*1昆明理工大学生命科学与技术学院,云南省高校靶点药物筛选与利用重点实验室,昆明650500;2云南省第一人民医院,昆明理工大学附属医院,昆明650032摘要SOS1(sonofsev ...上海营养与健康研究所 本站小编 Free考研考试 2022-02-13