不同浓度降钙素基因相关肽对小鼠海马区长时程抑制的作用
武鑫, 王冬慧, 孙宁宁, 苏少华, 张松江, 高剑峰^*
河南中医药大学基础医学院生理学学科，郑州 450046
摘要
本研究旨在探讨给予不同浓度降钙素基因相关肽(calcitonin gene-related peptide, CGRP)对小鼠海马区长时程抑制(long-term depression, LTD)的作用。将60只30日龄C57BL/6J雄性小鼠随机分为对照组、不同浓度(50, 100, 200 nmol/L) CGRP组、CGRP+CGRP8-37组以及CGRP+APV组，每组10只。用离体场电位方法检测外源性给予脑片不同浓度CGRP对小鼠海马区突触可塑性及LTD的作用。结果显示，与对照组相比，100和200 nmol/L CGRP可明显易化海马区LTD的诱发，差异具有显著性，并且CGRP可提高N-甲基-D-天门冬氨酸(N-methyl-D-aspartate, NMDA)受体介导的兴奋性突触后电流的幅度。CGRP的上述作用均可被CGRP选择性阻断剂CGRP8-37和NMDA受体阻断剂APV所阻断。以上结果提示，CGRP可剂量依赖性地易化小鼠海马区LTD的诱发，作用机制可能与NMDA受体功能的调节有关。


关键词： 降钙素基因相关肽; 海马区; 长时程抑制; 谷氨酸受体
分类号：R338.64

Effects of different concentrations of calcitonin gene-related peptide on long-term depression of hippocampus in mice
WU Xin, WANG Dong-Hui, SUN Ning-Ning, SU Shao-Hua, ZHANG Song-Jiang, GAO Jian-Feng^*
Department of Physiology, School of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China
Abstract
The purpose of this study was to explore the effects of calcitonin gene-related peptide (CGRP) on the long-term depression (LTD) of hippocampus in mice. Sixty C57BL/6J mice (30 days old) were randomly divided into control group, three CGRP (50, 100, and 200 nmol/L) groups, CGRP + CGRP8-37 group and CGRP + APV group (10 mice for each group). The effects of exogenous application of different concentrations of CGRP on synaptic plasticity and LTD in hippocampus of mice were detected by in vitro recording of local field potential. The results showed that higher doses (100 and 200 nmol/L) of CGRP significantly enhanced the induction of LTD in the hippocampus. Moreover, CGRP increased the magnitude of N-methyl-D-aspartate (NMDA) receptor-mediated excitatory postsynaptic currents. The above-mentioned effects of CGRP were blocked by either CGRP selective antagonist CGRP8-37 or NMDA receptor antagonist APV. These results suggest that CGRP can dose-dependently enhance the induction of LTD in hippocampus of mice, and the underlying mechanism involves the mediation of NMDA receptor function.


Key words: Calcitonin gene-related peptide; hippocampus; long-term depression; glutamate receptor

收稿日期：2019-03-12　　录用日期：2019-05-28
通讯作者：高剑峰　　E-mail: jc.gjfeng@hotmail.com
DOI: 10.13294/j.aps.2019.0076
引用本文：
武鑫, 王冬慧, 孙宁宁, 苏少华, 张松江, 高剑峰. 不同浓度降钙素基因相关肽对小鼠海马区长时程抑制的作用[J]. 生理学报 2019; 71 (6): 839-845.
WU Xin, WANG Dong-Hui, SUN Ning-Ning, SU Shao-Hua, ZHANG Song-Jiang, GAO Jian-Feng. Effects of different concentrations of calcitonin gene-related peptide on long-term depression of hippocampus in mice. Acta Physiol Sin 2019; 71 (6): 839-845 (in Chinese with English abstract).



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