巨噬细胞剔除可通过调控损伤骨骼肌炎症和氧化应激水平损害骨骼肌再生
刘晓光¹, 陈佩杰¹, 赵淋淋¹, 曾志刚^1,2, 肖卫华^1,*
1上海体育学院运动科学学院，上海 200438；²井冈山大学体育学院，吉安 343000
摘要
本研究旨在探索巨噬细胞在骨骼肌损伤修复中的作用及其机制。将小鼠随机分为损伤组、未损伤对照组、剔除组和剔除对照组。损伤组和剔除组小鼠用钝物击打构建骨骼肌挫伤模型，剔除组和剔除对照组小鼠用氯膦酸盐脂质体腹腔注射构建巨噬细胞剔除模型。骨骼肌钝挫伤后1、3、7和14 d取双侧腓肠肌，HE和Masson染色观察损伤骨骼肌再生和纤维化瘢痕愈合过程，real-time PCR及Western blotting检测骨骼肌中炎症因子、趋化因子和氧化应激因子表达变化。结果显示，骨骼肌损伤后14 d，损伤组组只存在少量再生肌纤维，而剔除组存在大量再生肌纤维，两组肌纤维直径差异具有显著性(P < 0.05)。伤后14 d，剔除组胶原纤维面积所占百分比显著高于损伤组(P < 0.01)。与未损伤对照组相比，损伤组多种促炎细胞因子、趋化因子和氧化应激因子表达均显著上调。与损伤组相比，剔除组中多种促炎细胞因子、趋化因子和氧化应激因子的表达在损伤后期(损伤后7~14 d)均显著增加。以上结果提示，骨骼肌损伤修复过程中多种炎症因子、趋化因子和氧化应激因子表达上调，剔除巨噬细胞后，上述因子的表达在损伤后期进一步上调，且骨骼肌再生能力受损，纤维化修复加剧。这些结果表明巨噬细胞在骨骼肌损伤修复过程中发挥了重要作用，炎症和氧化应激可能参与了剔除巨噬细胞损害骨骼肌再生这一过程。
关键词： 骨骼肌; 损伤修复; 巨噬细胞; 炎症因子; 趋化因子; 氧化应激因子
分类号：R339.4

Macrophages depletion impairs skeletal muscle regeneration by regulating inflammation and oxidative stress levels
LIU Xiao-Guang¹, CHEN Pei-Jie¹, ZHAO Lin-Lin¹, ZENG Zhi-Gang^1,2, XIAO Wei-Hua^1,*
1School of Kinesiology, Shanghai University of Sport, Shanghai 200438, China；²College of Physical Education, Jinggangshan University, Ji’an 343000, China
Abstract
The objective of this study was to explore the roles of macrophages in the regeneration of injured skeletal muscle and the mechanisms involved. Mice were randomly divided into the following groups: muscle contusion (S), muscle contusion control (SCon), macrophages depleted (T) and macrophages depleted control (TCon) groups. Muscle contusion model was created by high-energy blunt injury. Macrophages depletion model was constructed by injection of clodronate-liposomes. Their gastrocnemius muscles were harvested at the time points of 1, 3, 7 and 14 d post-injury. The changes in skeletal muscle morphology were assessed by hematoxylin- eosin (HE) staining and Masson’s trichrome staining. The mRNA and protein levels of inflammatory cytokines, chemokines and oxidative stress factors were analyzed by real-time polymerase chain reaction (RCR) and Western blotting, respectively. HE staining results showed that a small amount of regenerating myofibers were observed in the S group (14 d post-injury), whereas a large number of regenerating muscle fibers were observed in the T group. Quantitative analyses showed that the sizes of regenerating myofibers were significantly smaller in the T group as compared with the S group at 14 d post-injury (P < 0.05). At the same time, Masson staining results showed that macrophage depletion significantly increased the area of fibrosis as compared with the S group at 14 d post-injury (P < 0.01). The expression levels of inflammatory cytokines, chemokines, and oxidative stress factors were increased significantly after muscle injury. Moreover, macrophage depletion increased the expressions of inflammatory cytokines, chemokines and oxidative stress factors as compared with the S group during the later stage of injury (7–14 d post-injury). These results suggest that macrophages depletion can aggravate fibrosis and impair muscle regeneration, and inflammatory cytokines, chemokines and oxidative stress factors may be involved in this process.
Key words: Skeletal muscle;;;;;

收稿日期：2017-07-13　　录用日期：2018-03-01
通讯作者：肖卫华　　E-mail: xiaoweihua@sus.edu.cn
DOI: 10.13294/j.aps.2018.0003
引用本文：
刘晓光, 陈佩杰, 赵淋淋, 曾志刚, 肖卫华. 巨噬细胞剔除可通过调控损伤骨骼肌炎症和氧化应激水平损害骨骼肌再生[J]. 生理学报 2018; 70 (1): 23-32.
LIU Xiao-Guang, CHEN Pei-Jie, ZHAO Lin-Lin, ZENG Zhi-Gang, XIAO Wei-Hua. Macrophages depletion impairs skeletal muscle regeneration by regulating inflammation and oxidative stress levels. Acta Physiol Sin 2018; 70 (1): 23-32 (in Chinese with English abstract).



PDF全文下载地址:

点我下载PDF