在岗研究生导师情况介绍
所系名称计算生物学研究所
性别男
专业名称计算生物学
技术职务研究员
行政职务无
Mail地址gerwert@bph.rub
指导博士
生总数15指导硕士
生总数7已毕业学生通讯地址上海市岳阳路320号计算生物学研究所
目前博士
生数3目前硕士
生数2目前在校生邮政编码200031
研究方向生物物理学
研究工作BiophysicalChemistry
Proteinreactionmechanisms
MolecularDynamicSimulationsandQM/MM
Timeresolvedvibrationalspectroscopy
获奖情况Scientifichonours:
1990Heisenbergfellowship,DeutscheForschungsgemeinschaft
1991“KarlArnoldJansen“Awardof“Rheinisch-WestfälischeAkademieder
Wissenschaften”,Düsseldorf
1997Award“LeuchtturmprojektmolekulareBiophysik”ofNRW
2006Award„InnovationspreisRuhr2006“byMinisterpresidentNRW(Dr.J.Rüttgersand
Krupp
Foundation)
指导研究
生情况
个人简介Education:
1975-1981PhysicsatMuensterUniversity(“verygood;1.0”)
1982-1986Ph.D.studyBiophysicalChemistryatUniversityFreiburg
1986Dr.rer.nat.,UniversityofFreiburg,FacultyofChemistryandPharmacy
(“summacumlaude”)
1992“venialegendi”inPhysicalChemistry,BielefeldUniversity,Facultyof
Chemistry(Habilitation)
Positions:
1986-1990researchscientistatMax-Planck-InstituteinDortmund(Prof.BennoHess)
1990-1993Heisenberg-fellowoftheDeutscheForschungsgemeinschaftatMPIDortmund
andScrippsResearchInstitute,LaJolla,USA(Prof.PeterWright,Prof.Richard
Lerner)
Since1993ChairofBiophysics,FacultyofBiology
Since1994“Cooptation”inFacultyofPhysics,Ruhr-UniversityBochum
近期论文Prof.Dr.KlausGerwert
115)Warscheid,B.,Brucker,S.,Kallenbach,A.,Meyer,H.E.,Gerwert,K.andKötting,C.
Systematicapproachtogroup-specificisotopiclabelingofproteinsforvibrational
spectroscopy
submitted
114)Ollesch,J.,Künnemann,E.,Glockshuber,R.,Gerwert,K.
Prionprotein_-to-_transitionmonitoredbytime-resolvedFTIR
AppliedSpectroscopy,inpress
113)Kötting,C.andGerwert,K.
ProteinReactions:Resolvedwithtr-FTIR
SpectroscopyEurope,Vol.19,No.3,19-23(2007)
112)Kötting,C.,Kallenbach,A.,Suveyzdis,Y.,Eichholz,C.,Gerwert,K.
SurfacechangeofRasenablingeffectorbindingmonitoredinrealtimeatatomicresolution
ChemBioChem(8),781-787(2007)
111)Chakrabarti,P.,Daumke,O.,Suveyzdis,Y.,Kötting,C.,Gerwert,K.,Wittinghofer,A.
InsightintocatalysisofauniqueGTPasereactionbyacombinedbiochemicalandFTIR
approach
J.Mol.Biol.(367/4)983-995(2007)
110)HermesS,StachnikJM,OnidasD,RemyA,HofmannE,GerwertK
ProtonuptakeinthereactioncentermutantL210DNfromRhodobactersphaeroidesvia
protonatedwatermolecules
Biochemistry(45)13741-13748(2006)
109)Kötting,C.,Gerwert,K.
Proteinreaktionen:aufgelöstmittrFTIR
NachrichtenausderChemie(54)959-965(2006)
108)Kötting,C.,Blessenohl,M.,Suveyzdis,Y.,Goody,R.S.,Wittinghofer,A.,Gerwert,K.
AphosphoryltransferintermediateintheGTPasereactionofRasincomplexwithits
GTPase-activatingprotein
Proc.Natl.Acad.Sci,103,No.38,13911-13916(2006)
107)Garczarek,F.,Gerwert,K.
PolarisedFTIRSpectroscopyinConjunctionwithinsituH/DExchangereveals
theOrientationofProteinInternalCarboxylicAcids
JACS,128(1),28-29(2006)
106)Hermes,S.,Bremm,O.,Garczarek,F.,Derrien,V.,Liebisch,P.,Loja,P.,Sebban,P.,
Gerwert,K.,Haumann,M.
Atime-resolvedFe-specificX-rayabsorptionexperimentyieldsnoevidenceforaFe2+-
>Fe3+transitionduringQA-->QBelectrontransferinthephotosyntheticreactioncenter
Biochemistry,45,Vol.2,353-359(2006)
105)Garczarek,F.,Gerwert,K.
FunctionalWatersinProteinProtonTransfer
Nature,439,109-112(2006)
104)Kötting,C.,Gerwert,K.
MonitoringProtein-ProteinInteractionsbyTime-resolvedFTIRDifferenceSpectroscopy
In:Protein-ProteinInteractions;AmolecularcloningManual,2ndedition
Editors:Golemis,E.,Adams,P.
ColdSpringHarbor,NY,USA,279-299(2005)
103)Kötting,C.,Gerwert,K.
MonitoringProtein-LigandInteractionsbyTime-resolvedFTIRDifferenceSpectroscopy
In:Protein-LigandInteractions;MethodsinMolecularBiology
Editor:Nienhaus,U.
TheHumanaPressInc.,NJ,USA(2005)
102)Klähn,M.,Schlitter,J.,Gerwert,K.
TheoreticalIRSpectroscopyBasedonQM/MMCalculationsProvidesChangesinCharge
Distribution,BondLength,andBondAnglesoftheGTPLigandInducedbytheRas-Protein
BiophysicalJournal,88,3829-3844(2005)
101)Kötting,C.,Gerwert,K.
ProteinsinActionMonitoredbyTime-ResolvedFTIRSpectroscopy
ChemPhys.Chem,6,881-888(2005)
100)Schleicher,E.,Heßling,B.,Illarinova,V.,Bacher,A.,Weber,S.,Richter,G.,
Gerwert,K.
Light-inducedreactionsofEscherichiacoliDNAphotolyasemonitoredbyFouriertransform
infraredspectroscopy
FEBSJournal,272,1-12(2005)
99)Garczarek,F.,Brown,L.S.,Lanyi,J.K.,Gerwert,K.
Protonbindingwithinamembraneproteinbyaprotonatedwatercluster
Proc.Natl.Acad.SciUSA,Vol.102,No.10,3633-3638(2005)
98)Kandt,C.,Gerwert,K.,Schlitter,J.
WaterDynamicsSimulationasaToolforProbingProtonTransferPathways
inaHeptahelicalMembraneProtein
PROTEINS:Structure,Function,andBioinformatics,58,528-537(2005)
97)Kötting,C.,Gerwert,K.
Time-resolvedFTIRstudiesprovideactivationfreeenergy,activationenthalpyand
activationentropyforGTPasereactions
ChemicalPhysics,307,227-232(2004)
96)Kandt,C.,Schlitter,J.,Gerwert,K.
Replyto„ComplementariesandConvergenceofResultsinBacteriorhodopsin
TrimerSimulations“
BiophysicalJournal,87,1396-1396(2004)
95)Garczarek,F.,Wang,J.,El-Sayed,M.A.,Gerwert,K.
TheAssignmentoftheDifferentInfraredContinuumAbsorbanceChangesObservedinthe
3000-1800-cm–1RegionduringtheBacteriorhodopsinPhotocycle
BiophysicalJournal,87,2676-2682(2004)
94)Klähn,M.,Mathias,Kötting,C.,Nonella,M.,Schlitter,J.,Gerwert,K.,Tavan,P.
IRSpectraofPhosphateIonsinAqueousSolution:PredictionsofaDFT/MMApproach
ComparedwithObservations
J.Phys.Chem.A,108,6186-6194(2004)
93)Chakrabarti,P.,Suveyzdis,Y.,Wittinghofer,A.,Gerwert,K.,
FourierTransformInfraredSpectroscopyontheRap•RapGAPReaction,GTPaseActivation
withoutanArginineFinger
JournalofBiologicalChemistry,279,46226-46233(2004)
92)Hofmann,M.W.,Weise,K.,Ollesch,J.Agrawal,P.,Stalz,H.,Stelzer,W.,
Hulsbergen,F.,deGroot,H.,Gerwert,K.,Reed,J.,Langosch,D.
DeNovodesignofconformationallyflexibletransmembranepeptidesdrivingmembrane
fusion
Proc.Natl.Acad.SciUSA,Vol.101No.41,14776-14781(2004)
91)Remy,A.,NiklasJ.,KuhlH.,KellersP.,SchrottT.,RögnerM.,GerwertK.
FTIRspectroscopyshowsstructuralsimilaritiesbetweenphotosystemsIIfrom
cyanobacteriaandspinach
Eur.JournalofBiochemistry,271,563-567(2004)
90)Kandt,C.,Schlitter,J.,Gerwert,K.
DynamicsofWaterMoleculesintheBacteriorhodopsinTrimerinExplicitLipid/Water
Environment
BiophysicalJournal,86,705-717(2004)
89)Bourdos,N.,Gerwert,K.
ZeitaufgelöstetrFTIR-StudienzeigenProteineinAktion
Laborwelt,Nr.6,Vol.4,31-34(2003)
88)Remy,A.,Gerwert,K.
Couplingoflight-inducedelectrontransfertoprotonuptakeinphotosynthesis
NatureStruct.Biol.,10,637–644(2003)
87)Remy,A.,Boers,R.B.,Egorova-Zachernyuk,T.,Gast,P.,Lugtenburg,J.,
Gerwert,K.
Doesdifferentorientationofthemethoxygroupsofubiquinone-10inthereactioncentreof
RhodobactersphaeroidescausedifferentbindingatQAandQB?
EuropeanJournalofBiochemistry,3603-3609(2003)
86)Gerwert,K.
Proteinsatworkmonitoredbytime-resolvedFTIR-differencespectroscopy
BiophysicalJournal,82,36(2002)
85)Gerwert,K.
GTPasemechanismofRasstudiedbytime-resolvedFTIRspectroscopy.
AbstractsOfPapersOfTheAmericanChemicalSociety,223,039-PhysPt.2(2002)
84)Kauffmann,E.,Austin,R.H.,Gerwert,K.
Probingproteinfoldingwithtime-resolvedFTIRspectroscopy
Noveltechniquesfortheinvestigationofchangesinproteinconformation
B.I.F.Futura,16,152-158(2001)
83)Gerwert,K.
MolecularReactionMechanismsofProteinsMonitoredbyTime-resolvedFTIRDifference
Spectroscopy
In:"HandbookofVibrationalSpectroscopy,5-ApplicationsofVibrationalSpectroscopyin
LifeandNaturalSciences"
J.M.Chalmers,P.R.Griffiths,Eds.,JohnWiley&SonsLtd.,Chichester(2001)
82)Spassov,V.Z.,Luecke,H.,Bashford,D.,Gerwert,K.,
pKαCalculationsSuggestStorageofanExcessProtoninaHydrogen-BondedWater
NetworkinBacteriorhodopsin.
JournalofMolecularBiology,312,203-219(2001)
81)Radzwill,N.,Gerwert,K.,Steinhoff,H.-J.
Time-ResolvedDetectionofTransientMovementofHelicesFandGinDoublySpin-
LabeledBacteriorhodopsin.
BiophysicalJournal,80,2856–2866(2001)
80)Kauffmann,E.,Darnton,N.C.,Austin,R.H.,Batt,C.,Gerwert,K.
Lifetimesofintermediatesintheβ-sheettoα-helixtransitionofβ-lactoglobulinbyusinga
diffusionalIRmixerIRmixer.
Proc.Natl.Acad.SciUSA,98,6646-6649(2001)
79)Allin,C.,Ahmadian,M.R.,Wittinghofer,A.,Gerwert,K.
MonitoringtheGAPCatalyzedH-Rasp21GTPaseReactionatAtomicResolutioninReal
Time.
Proc.Natl.Acad.SciUSA,98,7754–7759(2001)
78)Allin,C.,Gerwert,K.
RasCatalyzesGTPHydrolysisbyShiftingNegativechargeesfromγ-toβ-Phosphateas
revealedbyTime-resolvedFTIRDifferenceSpectroscopy
Biochemistry,40,3037–3046(2001)
77)Brudler,R.,Rammelsberg,R.,Woo,T.T.,Getzoff,E.D.,Gerwert,K.
StructureoftheI1earlyintermediateofphotoactiveyellowproteinbyFTIRspectroscopy.
NatureStructuralBiology,8,no.3,265–270,(2001)
76)Brudler,Ronald,Meyer,TerenceE.,Genick,UlrichK.Devanathan,Woo,TammyT.,Millar,
DavidP.,Gerwert,Klaus,Cusanovich,MichaelA.,Tollin,Gordon,Getzoff,ElizabethD.
CouplingofHydrogenBondingtoChromophoreConformationandFunctioninPhotoactive
YellowProtein.
Biochemistry,39,13478-13486(2000)
75)Gerwert,K.
MolecularReactionMechanismsofProteinsMonitoredbyTime-ResolvedFTIRandFTRaman
Spectroscopy.In:InfraredAnalysisofPeptidesandProteins(Singh,BalRam,Ed.)
AmericanChemicalSociety,WashingtonD.C.,159-167(2000).
ACSSymposiumSeries,Vol.750.
74)Gerwert,K.
Time-ResolvedFT-IRDifferenceSpectroscopy:ATooltoMonitorMolecularReactionof
Proteins.In:InfraredandRamanSpectroscopyofBiologicalMaterials(Gremlich,H.-U.,Yan,
B.eds.)
MarcelDekker,Inc.,NewYork,6,193-230(2000)
73)Rink,Th.,Pfeiffer,M.,Oesterhelt,D.,Gerwert,K.,Steinhoff,H.-J.
UnravelingPhotoexcitedConformationalChangesofBacteriorhodopsinbyTimeResolved
EPRSpectroscopy,
BiophysicalJournal,78,1519-1530(2000)
72)Devanathan,S.,Brudler,R.,Heßling,B.,Woo,T.T.,Gerwert,K.,Getzoff,E.D.,Cusanovich,
M.A.,Tollin,G.
DualPhotoactiveSpeciesinGlu46AspandGlu46AlaMutantsofPhotoactiveYellow
Protein:apHDrivenColorTransition,
Biochemistry,38,13766-13772(1999)
71)Egorova-Zachernyuk,T.,Remy,A.,Shkuropatov,A.,Gast,P.,Hoff,A.,Gerwert,K.,de
Groot,H.
EfficientConditionsforthePhotoaccumulationofI-inthePhotosyntheticReactionCentres
ofRhodobactersphaeroidesR26withUniformlyLabelledBacteriopheophytinMonitoredby
FourierTransformInfraredDifferenceSpectroscopy,
VibrationalSpectroscopy,19,347-352(1999)
70)Steinhoff,H.-J.,Pfeiffer,M.,Rink,Th.,Burlon,O.,Kurz,M.,Riesle,J.,Heuberger,E.,
Gerwert,K.,Oesterhelt,D.
AzideReducestheHydrophobicBarrieroftheBacterorhodopsinProton
Channel
BiophysicalJournal,76,2702-2710(1999)
69)Rammelsberg,R.,Gerwert,K.
Time-ResolvedStep-ScanFT-IRSpectroscopyofNoncyclicReactions.
Proceedingsofthe„TwelfthInternationalConferenceonFourierTransformSpectroscopy“
WasedaUniversityEnterprisePress,inpress.
68)Gerwert,K.
MolecularReactionMechanismsofProteinsMonitoredbyTime-ResolvedFTIRSpectroscopy,
Biol.Chem.,380,931-935(1999)
67)Gerwert,K.
Biologie2000(1999)
66)Gerwert,K.
ProteineinAktion
BIORUBIN(1999)
65)Pfeiffer,M.,Rink,Th.,Gerwert,K.,Oesterhelt,D.,Steinhoff,H.-J.
Site-directedSpin-labelingRevealstheOrientationoftheAminoAcidSide-chainsintheE-F
Loop
JournalofMolecularBiology,287,163-171(1999)
64)Rammelsberg,R.,Boulas,S.,Chorongiewski,H.,Gerwert,K.
Set-upfortime-resolvedstep-scanFTIRspectroscopyofnoncyclicreactions
VibrationalSpectroscopy,654(1999)
63)Lübben,M.,Prutsch,A.,Mamat,B.,Gerwert,K.
ElektrontransferinducessidechainconformationalchangesofGlu-286fromcytochrome
bo3
Biochemistry,38,2048-2056(1999)
62)Cepus,V.,Scheidig,A.J.,Goody,R.S.,Gerwert,K.
Time-resolvedFTIRstudiesoftheGTPasereactionofH-rasp21revealakey
rolefortheβ-phosphate.
Biochemistry,37,10263-10271(1998)
61)Rammelsberg,R,Huhn,G.,Lübben,M.,Gerwert,K.
Bacteriorhodopsin’sIntramolecularProton-ReleasePathwayConsistsofaHydrogen-
BondedNetwork
Biochemistry,37,5001-5009(1998)
60)Brudler,R.,Gerwert,K.
Step-scanFTIRspectroscopyoftheQA-QB→QAQB-transitioninRb.sphaeroidesR26
reactioncentres
PhotosynthesisResearch,1-6(1998)
59)Cepus,V.,Ulbrich,C.,Allin,Chr.,Troullier,A.,Gerwert,K.
FTIRphotolysisstudiesofcagedcompounds
MethodsinEnzymologyedsMariott,291,223-245(1998)
58)Gerwert,K.
Proteinsatwork:Time-resolvedFTIRstudiesofbacteriorhodopsinandtheGTP-binding
proteinP21.
In:InteractingProteinDomainsTheirRoleinSignalandEnergyTransduction(Heilmeyer,
L.,ed.)Springer-Verlag,Berlin,63-72(1997),
NATOASISeries,Vol.H102
57)Gerwert,K.
ZeitaufgelösteFTIR-SpektroskopiezurBestimmungmolekularerReaktionsmechanismen
vonProteinen
Biospektrum2,34-39(1997)
56)Rink,Th.,Riesle,J.,Oesterhelt,D.,Gerwert,K.,Steinhoff,H.-J.
SpinlabelingstudiesoftheconformationalchangesinthevicinityofD36,D38,T46andE161
ofbacteriorhodopsinduringthephotocycle
BiophysicalJournal,73,983-993(1997)
55)Rüdiger,M.,Tittor,J.,Gerwert,K.,Oesterhelt,D.
ReconstitutionofBacteriorhodopsinfromtheApoproteinandRetinalstudiedbyFouriertransform
InfraredSpectroscopy
Biochemistry,36,4867-4874(1997)
54)Rammelsberg,R.,Heßling,B.,Chorongiewski,H.,Gerwert,K.
Molecularreactionmechanismsofproteinsmonitoredbynanosecondstep-scanFT-IR
differencespectroscopy
AppliedSpectroscopy,51,558-562(1997)
53)Heßling,B.,Herbst,J.,Rammelsberg,R.,Gerwert,K.
FourierTransformInfraredDouble-FlashExperimentsResolveBacteriorhodopsin’sM1to
M2Transition
BiophysicalJournal,73,2071-2080(1997)
52)Lübben,M.,Gerwert,K.
Redox-FTIRdifferencespectroscopyusingcagedelectronsrevealscontributionofcarboxyl
groupstothecatalyticmechanismofhaemcopperoxidases
FEBSLetters,397,303-307(1996)
51)leCoutre,J.,Gerwert,K.
KineticIsotopeeffectsrevealanice-likeandaliquid-phase-typeintramolecularproton
transferinBacteriorhodopsin
FEBSLetters,398,333-336(1996)
50)Troullier,A.,Gerwert,K.,Dupont,Y.
AtimeresolvedFTIRdifferencespectroscopystudyoftheSRCa2+-ATPase:kineticsofthe
highaffinitycalciumbindingatlowtemperature
BiophysicalJournal,71,2970-2983(1996)
49)Brudler,R.,deGroot,H.J.M.,vanLiemt,W.B.S.,Gast,P.,Hoff,A.J.,Lugtenburg,J.,
Gerwert,K.
FTIRspectroscopiccharacterizationoftheQAandQBbindingsitesinRhodobacter
sphaeroidesR26reactioncenters.
In:TheReactionCenterofPhotosyntheticBacteria(Michel-Beyerle,M.-E.,ed.)Springer-
Verlag,Berlin,395-404(1996)
48)Gerwert,K.
WiefunktionierenProteine?
RUBIN2(1995)
47)Brudler,R.,deGroot,H.J.M.,vanLiemt,W.B.S.,Gast,P.,Hoff,A.J.,Lugtenburg,J.,
Gerwert,K.
FTIRspectroscopyshowsweaksymmetrichydrogenbondingoftheQBcarbonylgroupsin
RhodobactersphaeroidesR26reactioncentres
FEBSLetters,370,88-92(1995)
46)Gerwert,K.
„Investigationoffastreactionsandintermediates“
In:InfraredandRamanSpectroscopy(editorB.Schrader),S.617-637,VCHWeinheim
(1995)
45)Engels,M.,Gerwert,K.,Bashford,D.
ComputationalStudiesonEarlyIntermediatesofBacteriorhodopsin
Photocycle
BiophysicalChemistry,56,95-104(1995)
44)Rüdiger,M.,Haupts,U.,Gerwert,K.,Oesterhelt,D.
Chemicalreconstitutionofachloridepumpinactivatedbyasinglepointmutation
EMBOJ.,14,1599-1606(1995)
43)leCoutre,J.,Rüdiger,M.,Oesterhelt,D.,Gerwert,K.
FTIRinvestigationofthebluetoacid-purpletransitionofBacteriorhodopsin
byuseofinducedhalidebinding
JournalofMolecularStructure,349,165-168(1995)
42)leCoutre,J.,Tittor,J.,Oesterhelt,D.,Gerwert,K.
Experimentalevidenceforhydrogen-bondednetworkprotontransferin
bacteriorhodopsinshownbyFTIRspectroscopyusingazideascatalyst
Proc.Natl.Acad.SciUSA,92,4962-4966(1995)
41)Brudler,R.,deGroot,H.J.M.,vanLiemt,W.B.S.,Steggerda,W.F.,Esmeijer,
R.,Gast,P.,Hoff,A.J.,Lugtenburg,J.,Gerwert,K.
Asymmetricbindingofthe1-and4-C=OgroupsofQAinRhodobacter
sphaeroidesR26reactioncentresmonitoredbyFouriertransforminfra-red
spectroscopyusingsite-specificisotopicallylabelledubiquinone-10
EMBOJ.,13,5523-5530(1994)
40)leCoutre,J.,Gerwert,K.
HowcatalizesAzideProton-PumpinginD96NMutatedBacteriorhodopsin
TimeResolvedVibrationalSpectroscopyVI(Lau,A.,Siebert,F.,Werncke,W.,eds.)
Springer-Verlag,Berlin,256-257(1994)
39)Gerwert,K.,Cepus,V.,Scheidig,A.,Goody,R.S.
Time-resolvedFTIR-StudiesofH-RasP21GTPaseActivity
TimeResolvedVibrationalSpectroscopyVI(Lau,A.,Siebert,F.,Werncke,
W.,eds.)Springer-Verlag,Berlin,185-187(1994)
38)Gerwert,K.
MolekulareReaktionsmechanismenphotosynthetischerMembranproteine
FUTURA1,13-17(1993)
37)Gerwert,K.,Souvignier,G.
ZeitaufgelösteFTIR-Differenzspektroskopie
Nachr.Chem.Tech.Lab.41(9),950-956(1993)
36)Heßling,B.,Souvignier,G.,Gerwert,K.
AModel-IndependentApproachtoAssigningBacteriorhodopsin's
IntramolecularReactionstoPhotocycleIntermediates
BiophysicalJournal,65,1929-1941(1993)
35)Gerwert,K.
Molecularreactionmechanismofproteinsasmonitoredbytimeresolved
FTIRspectroscopy
CurrentOpinionStructuralBiology,3,769-773(1993)
34)Gerwert,K.
Time-ResolvedFTIRStudiesonBacteriorhodopsin
TimeResolvedVibrationalSpectroscopyV(Takahashi,H.,ed.)
Springer-VerlagBerlin-Heidelberg,1992
33)leCoutre,J.Gerwert,K.
Theazide-effectinD966N/Gmutatedbacteriorhodopsinsmonitored
bytimeresolvedFTIR-difference-spectroscopy
In:StructuresandFunctionsofRetinalProteins,J.L.Rigaud(Ed.)
ColloqueINSERM221,127-130,Paris(1992)
32)Heßling,B.,Souvignier,G.,Gerwert,K.
Anewapproachtoanalysekineticdataofbacteriorhodopsin,factor
analysisanddecomposition
In:StructuresandFunctionsofRetinalProteins,J.L.Rigaud(Ed.)
ColloqueINSERM221,155-158,Paris(1992)
31)Souvignier,G.,Gerwert,K.
TheprotonuptakereactionsofBacteriorhodopsindeterminedby
time-resolvedstroboscopic-FTIR-spectroscopy
BiophysicalJournal,63,1393-1405(1992)
30)Gerwert,K.
Molecularreactionmechanismofphotosyntheticproteinsas
determinedbyFTIR-spectroscopy
BiochimicaetBiophysicaActa,1101,147-153(1992)
29)Gerwert,K.,Souvignier,G.
Time-resolvedFTIRstudiesonproteins
Proceedingsofthe8thInternationalConferenceonFTIRSpectroscopy
SPIE,1575,431-432(1992)
28)Bashford,D.,Gerwert,K.
ElectrostaticcalculationsofthepKa'sofionazablegroupsin
Bacteriorhodopsin
JournalofMolecularBiology,224,473-486(1992)
27)Buchanan,S.,Michel,H.,Gerwert,K.
Light-inducedchargeseparationinRps.viridisreactioncentersmonitored
byFTIRdifferencespectroscopy:theQuinonevibrations
Biochemistry,31,1314-1322(1992)
26)Gerwert,K.,Hoffmann,A.,Schrader,B.
FT-RamanLight-InducedIntermediateSpectraofPhotobiologicalSystems
Proceedingsofthe13.
InternationalConferenceonRamanSpectroscopy,
(Kiefer,W.,Cardona,M.,Schaack,G.,Schneider,F.W.,andSchrötter,H.W.,eds.)490-491
(1992),JohnWiley&Sons,Chichester
25)Buchanan,S.,Michel,H.,Gerwert,K.
InvestigationofquinonereductioninRps.viridisbyFTIRdifferencespectroscopy
andX-raydiffractionanalysis
In:ReactionCentersofPhotosyntheticBacteria
(M.E.Beyerle,ed.)SpringerSeriesinBiophysics6,75-85(1990)
24)Gerwert,K.,Hoffmann,A.,Schrader,B.
ComparisonofFTIRandFTRamanSpectraofDark-AdaptedandLight-Adapted
Bacteriorhodopsin
Proceedingsofthe12.InternationalConferenceonRamanSpectroscopy,
(Durig,J.R.,Sullivan,J.F.,eds.)852-853(1990),JohnWiley&Sons,Chichester
23)Gerwert,K.,Souvignier,G.,Hess,B.
TimeresolvedFTIRdifferencespectroscopyappliedtoultrathinlayers
ofmembraneproteins.
SPIE,Proceedingsofthe7.InternationalConferenceonFTIR
Spectroscopy,1145,130-131(1990)
22)Gerwert,K.,Souvignier,G.,Hess,B.
Simultaneousmonitoringoflight-inducedchangesinproteinside-group
protonation,chromophore,isomerization,andbackbonemotionof
bacteriorhodopsinbytime-resolvedFourier-transforminfraredspectroscopy
Proc.Natl.Acad.SciUSA,87,9774-9778(1990)
21)Buchanan,S.,Michel,H.,Gerwert,K.
FTIRstudiesoflightinducedintramolecularprocessesoncrystalsand
reconstitutedreactioncentersfromrhodopseudomonasviridis
In:CurrentResearchinPhotosynthesis,
(Baltschelsky,M.,ed.)Kluwer,AcademicPublishers,Vol.I,pp.I.1.69-72
(1990)
20)Gerwert,K.,Hess.B.,Engelhard,M.
Prolineresiduesundergostructuralchangesduringprotonpumping
inbacteriorhodopsin
FEBSLett.,261,449-459(1990)
19)Gerwert,K.,Hess,B.,Soppa,J.,Oesterhelt,D.
TheroleofAsp96intheprotonpumpmechanismofbacteriorhodopsin
Proc.Natl.Acad.SciUSA,86,4943-4947(1989)
18)Gerwert,K.
IntramolekulareProteindynamik.UntersuchungenmitHilfezeitaufgelöster
Fourier-Transform-Infrarot-Spektroskopie
FUTURA1,31-36(1988)
17)Gerwert,K.,Hess,B.
Lightinducedintramolecularprocessesofbacteriorhodopsinmonitored
bytimeresolvedFTIR(Fouriertransforminfrared)spectroscopy
In:TheIonPumps:Structure,Function,andRegulation
(W.D.Stein,ed.),AlanR.Liss,Inc.,NewYork,pp.321-326(1988)
16)Gerwert,K.,Hess,B.
Initiationoflight-inducedprotontransfersinbacteriorhodopsinmonitored
byFTIRdifferencespectroscopy
In:BiophysicalStudiesofRetinalProteins.ProceedingsofaConferenceinMemoryof
LauraEisenstein(Th.G.Ebrey,H.Frauenfelder,B.Honigand
K.Nakanishi,eds.),UniversityofIllinoisPress,pp.144-148(1988)
15)Gerwert,K.,Hess,B.,Michel,H.,Buchanan,S.
FTIRstudiesoncrystalsofphotosyntheticreactioncenters
FEBSLett.,232,303-307(1988)
14)Gerwert,K.,Hess,B.
InvestigationofIntramolecularProcessesinProteinsbyTimeResolvedFTIRDifference
Spectroscopy
Mikrochim.ActaI,255-258(1988)
13)Gerwert,K.
IntramolekulareProteindynamikuntersuchtmitzeitaufgelösterFourier
TransformInfrarot-Differenzspektroskopie
Ber.Bunsenges.Phys.Chem.,92,978-982(1988)
12)Gerwert,K.,Ganter,U.M.,Siebert,F.,Hess,B.
Onlywater-exposedcarboxylgroupsareprotonatedduringthetransition
tothecation-freebluebacteriorhodopsin
FEBSLett.,213,39-44(1987)
11)Gerwert,K.,Hess,B.
IntramolecularprotontransfersinbacteriorhodopsinmonitoredbyFourier
transforminfrareddifferencespectroscopy
In:InteractionsofWaterinIonicandNonionicHydrates(H.Kleeberg,ed.),
Springer-VerlagBerlin-Heidelberg,pp.183-186(1987)
10)Gerwert,K.,Hess,B.
InvestigationoftheMtoBRbackreactioninbacteriorhodopsinbytime
resolvedFTIRdifferencespectroscopy
In:SpectroscopyofBiologicalMolecules-NewAdvances(E.D.Schmid,
F.W.Schneider,andF.Siebert,eds.),JohnWiley&Sons,Chichester,
NewYork,Brisbane,Toronto,Singapore,pp.261-263(1987)
9)Gerwert,K.,Buchanan,S.,Hess,B.,Michel,H.
FTIRspectroscopyoncrystalsofthephotosyntheticreactioncentersfrom
RhodopseudomonasviridisusinganIRmicroscope.
In:SpectroscopyofBiologicalMolecules-NewAdvances(E.D.Schmid,F.W.Schneider,
andF.Siebert,eds.),JohnWiley&Sons,Chichester,NewYork,Brisbane,Toronto,
Singapore,
pp.301-303(1987)
8)Gerwert,K.,Siebert,F.
Evidenceforlight-induced13-cis,14-s-cis,isomerizationinbacteriorhodopsin
obtainedbyFTIRdifferencespectroscopyusingisotopicallylabelledretinals
EMBOJournal,5,805-811(1986)
7)Gerwert,K.,Siebert,F.,Pardoen,J.A.,Winkel,C.,Lugtenburg,J.
Vibrationalanalysisofchromophor-configurationsinbacteriorhodopsin
duringthephotocycleusingFTIRdifferencespectrscopy.
In:SpectroscopyofBiologicalMolecules(A.J.P.Alix,L.Bernardand
M.Manfait,eds.),JohnWiley&Sons,Chichester,NewYork,Brisbane,
Toronto,Singapore,pp.373-375(1985)
6)Gerwert,K.,Siebert,F.,Pardoen,J.A.,Winkel,C.,Lugtenburg,J.
EvidencefromFTIR-measurementsforaseparationofthe
protonatedSchiffbasefromthecounterionintoalesspolarenvironment.
In:Time-ResolvedVibrationalSpectroscopy(A.Laubereauand
M.Stockburger,eds.),Springer-Verlag,Berlin,Heidelberg,NewYork,
Tokyo,pp.259-262(1985)
5)Gerwert,K.,Rodriguez-Gonzales,R.,Siebert,F.
Time-resolvedinfraredspectroscopyappliedtophotobiological
systems.
In:Time-ResolvedVibrationalSpectroscopy(A.Laubereauand
M.Stockburger,eds.),Springer-VerlagBerlin,Heidelberg,NewYork,
Tokyo,pp.259-262(1985)
4)Engelhard,M.,Gerwert,K.,Hess,B.,Kreutz,W.,Siebert.F.
Light-drivenprotonationchangesofinternalasparticacidsofbacteriorhodopsin:
Aninvestigationbystaticandtime-resolvedinfrareddifference
spectroscopyusing4−13Casparticacidlabeledpurplemembrane
Biochemistry,24,400-407(1985)
3)Gerwert,K.,Kreutz,W.,Mäntele,W.,Siebert,F.,Engelhard,M.,Hess,B.
Theroleofcarboxylicacidresiduesintheprotonpumpmechanismof
bacteriorhodopsin(bR):InvestigationbytimeresolvedandstaticIR
differencespectroscopywith4−13Clabelledasparticacid.
Photochem.andPhotobiol.,39,PergamonPress,MPM-C18
(1984)
2)Siebert,F.,Mäntele,W.,Gerwert,K.
Fourier-transforminfraredspectroscopyappliedtorhodopsin.Theproblemof
theprotonationstateoftheretinylideneSchiffbasereinvestigated
Eur.JournalofBiochemistry,136,119-127(1983)
1)Gerwert,K.,Kollath,K.J.
Measurementofphotoionisationcrosssectionsforthe72
32Pand62
32Dexcitedstatesof
caesium
J.Phys.B.:At.Mol.Phys.,16,L217-L221(1983)
