周燕
生物化工专业：博士生导师/硕士生导师
生物工程与技术专业：博士生导师/硕士生导师
生物工程专业：工程硕士生导师
现任华东理工大学生物反应器工程国家重点实验室教授
1988年获华东化工学院（现华东理工大学）生物化学专业理学学士学位；1991年获华东理工大学生物化工专业工学硕士学位；毕业后留校任教，期间曾在香港城市大学生物及化学系开展研究工作。现任华东理工大学生物反应器工程国家重点实验室教授，博士生导师，动物细胞与组织工程研究室副主任。
多年来一直从事动物细胞培养技术研究及其在干细胞体外培养和人体组织体外构建中的应用，研究工作聚焦于动物细胞体外培养的无血清培养基研制、基于代谢工程的动物细胞大规模无血清高密度悬浮培养技术、采用生物反应器及微载体细胞培养体系生产病毒疫苗、干细胞体外规模化扩增和定向诱导分化、工程化人体组织体外构建新技术及生物反应器研制。
先后主持并完成了国家“863”项目“组织工程种子细胞体外大量扩增技术研究”、“临床级干细胞产业化技术研发”，国家重大新药创制课题“哺乳动物细胞大规模培养及药物制备关键技术”，国家支撑计划项目“动物细胞大规模无血清培养及表达产品制备技术”，国家自然科学基金项目“微组织体外构建技术及其过程分析”、“基于微构重塑技术构建大型预血管化工程组织”，上海市重大科研攻关课题“骨髓间充质干细胞体外扩增及成骨诱导的应用研究”、“功能性聚酯基组织工程材料制备及其对组织再生调控的研究”、“hiHep体外规模化扩增”、“微载体技术用于兽用细胞疫苗产业化研究”、“猪流行性腹泻、猪传染性胃肠炎和轮状病毒的诊断及三联苗研制”等，其中“猪流行性腹泻、猪传染性胃肠炎和轮状病毒的诊断及三联苗研制”获上海市科技进步奖二等奖。在国内外核心刊物发表论文七十余篇，申请、公开或授权国家发明专利十多项。
研究方向
1、 干细胞/成体细胞体外大规模扩增与分化诱导技术
研究和认识体外培养过程中培养环境对干细胞增殖和定向诱导分化的影响及其规律，建立干细胞在生物反应器系统中模拟体内生长环境、维持细胞特性与功能的规模化培养技术，实现干细胞体外大量扩增。
2、 细胞三维培养技术及其共培养模型建立
模拟细胞体内生长和生存的微环境，研究并认识细胞与细胞、细胞与胞外基质、细胞与细胞因子、细胞与支架材料的相互作用和相互关系，研制细胞生长和组织构建的支架材料及三维支架，建立不同细胞的三维共培养模型。
3、 人体组织体外构建技术及其生物反应器
从降低工程化组织体外构建过程中的传质限制出发，研究培养环境、流体流动、物质传递对细胞生长、组织发育的影响及机制，研制工程化组织体外构建的生物反应器，建立细胞、胞外基质和血管化网均布以及过程参数易控的大型预血管化工程组织体外构建新技术。
4、 动物细胞大规模无血清悬浮培养技术
研究无血清培养基的组成和成分对细胞生长、代谢及产物表达的影响，通过无血清培养基设计和细胞培养培养过程优化，调控细胞生长和代谢，建立适用于哺乳动物细胞大规模培养过程的无血清培养基开发技术和哺乳动物细胞大规模无血清高密度流加培养技术。
5、基于生物反应器的细胞培养和病毒疫苗生产技术
研究培养环境及操作参数对细胞生长的影响，认识细胞生理状态与病毒感染、复制的关系，设计和开发适合于动物细胞大规模培养与病毒扩增的培养基组成，建立高效、稳定的病毒规模化生产技术。
近年来代表性论著
1. Wei Liu, Mi Zhang, Miaomiao Zhou, Ce Gu, Zhaoyang Ye, Yan Xiao*, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and evaluation of modified poly(ethylene terephthalate) microfibrous scaffolds for hepatocyte growth and functionality maintenance. Materials Science & Engineering C-Materials for Biological Applications. 2020; 109:110523.
2. Wei Liu, Mi Zhang, Yan Xiao, Zhaoyang Ye, Yan Zhou*, Meidong Lang, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on galactosylated poly(ethylene terephthalate) microfibrous scaffolds. Biochemical Engineering Journal. 2020; 160:107565.
3. Ce Gu, Miaomiao Chai, Jiaxing Liu, Hui Wang, Wenjing Du, Yan Zhou*, Wen-Song Tan. Expansion of transdifferentiated hepatocyte in a serum-free microcarrier culture system. Digestive Diseases and Sciences. 2020; 65(7): 2009-2023.
4. Jiaxing Liu, Hui Wang, Wenxia Ren, Yan Zhou*, Wen-Song Tan. β-mercaptoethanol promotes osteogenesis of human mesenchymal stem cells via sirt1-ERK pathway. Cytotechnology. 2020; https://doi.org/10.1007/s10616-020-00412-9.
5. Miaomiao Chai, Ce Gu, Qihua Shen, Jiaxing Liu, Yi Zhou, Ziyang Jin, Wanli Xiong, Yan Zhou*, Wensong Tan. Hypoxia alleviates dexamethasone-induced inhibition on angiogenesis in co-culture of HUVECs and rBMSCs via HIF-1α. Stem Cell Research & Therapy. 2020; https://doi.org/10.1186/s13287-020-01853-x.
6. Qihua Shen, Xiaoting Fan, Mingli Jiang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture. Archives of Biochemistry and Biophysics.2019; 675: 108108. DOI: 10.1016/j.abb.2019.108108.
7. Huimin He, Zhaoyang Ye*, Yan Zhou*, Wensong Tan. Dynamic formation of cellular aggregates of chondrocytes and mesenchymal stem cells in spinner flask. Cell Proliferation. 2019; e12587. DOI: 10.1111/cpr.12587.
8. Ce Gu, Panpan Li, Wei Liu, Yan Zhou*, Wen-Song Tan. The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium. Journal of Cellular and Molecular Medicine. 2019; 23(6): 4165-4178.
9. Jie Sun, Yan Zhou*, Zhaoyang Ye*, Wen-Song Tan. Transforming growth factor-β1 stimulates mesenchymal stem cell proliferation by altering cell cycle through the FAK-Akt-mTOR pathway. Connective Tissue Research. 2019; 60(4): 406-417.
10. Anmin Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Bioactive Poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration. Journal of Biomaterials Applications. 2019; 33(9): 1242-1251.
11. Wei Liu, Dan Hu, Ce Gu, Yan Zhou*, Wen-Song Tan. Fabrication and in vitro evaluation of a packed-bed bioreactor based on an optimum two-stage culture strategy. Journal of Bioscience and Bioengineering. 2019; 127(4): 506-514.
12. Pan-Pan Li, Ce Gu, Bing-Yu Liang, Lin Wang, Yan Zhou*, Wen-Song Tan. A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes. Cytotechnology. 2019; 71(1): 329-344.
13. Yan Xiao*, Miaomiao Zhou, Mi Zhang, Wei Liu, Yan Zhou*, Meidong Lang*. Hepatocyte culture on 3D porous scaffolds of PCL/PMCL. Colloids and Surfaces B-Biointerfaces. 2019; 173: 185-193.
14. Mengjuan Liu, Jiaqi Wang, Hongping Tang, Li Fan, Liang Zhao, Hai-Bin Wang, Yan Zhou*, Wen-Song Tan. Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody. Biotechnology Letters. 2018; 40(11-12): 1487-1493.
15. Xiaoting Fan, Yi Teng, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs. Journal of Cell Science. 2018; 131(13): jcs216135. DOI: 10.1242/ jcs.216135.
16. Fan Xiaoting, Li Lei, Ye Zhaoyang*, Zhou Yan*, Tan Wensong. Regulation of osteogenesis of human amniotic mesenchymal stem cells by sodium butyrate. Cell Biology International. 2018; 42(4): 457-469.
17. Shiwei Miao, Panpan Xie, Mao Zou, Li Fan, Xuping Liu, Yan Zhou, Liang Zhao, Ding Ding, Haibin Wang, Wen-Song Tan*. Identification of multiple sources of the acidic charge variants in an IgG1 monoclonal antibody. Applied Microbiology and Biotechnology. 2017; 101(14): 5627-5638.
18. Liu Yanli, Xu Jiazhu, Zhou Yan, Ye Zhaoyang*, Tan Wensong. Layer-by-layer assembled polyelectrolytes on honeycomb-like porous poly(ε-caprolactone) films modulate the spatial distribution of mesenchymal stem cells. Materials Science & Engineering: C-Materials for Biological Applications. 2017; 78: 579-588.
19. Zhang Songjie, Zhou Min, Ye Zhaoyang*, Zhou Yan*, Tan Wensong. Fabrication of viable and functional pre-vascularized modular bone tissues by coculturing MSCs and HUVECs on microcarriers in spinner flasks. Biotechnology Journal. 2017; 12(8): **. DOI: 10.1002/biot. .
20. Zhang Yan*, Du Xiaolin, Hu Dan, Zhang Jing, Zhou Yan, Min Guoquan, Lang Meidong*. Combined chemical groups and topographical nanopattern on the poly(epsilon-Caprolactone) surface for regulating human foreskin fibroblasts behavior. ACS Applied Materials & Interfaces. 2016; 8(12): 7720-7728.
21. Chen Min, Xu Lei, Zhou Yan, Zhang Yan, Lang Meidong, Ye Zhaoyang*, Tan Wensong*. Poly(ε-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis. Cell Proliferation. 2016; 49(4): 512-522.
22. Panpan Xie, Huijie Niu, Xinning Chen, Xintao Zhang, Shiwei Miao, Xiancun Deng, Xuping Liu, Wen-Song Tan, Yan Zhou*, Li Fan*. Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures. Applied Microbiology and Biotechnology. 2016; 100(24): 10343-10353.
23. Lei Xu, Yuxi Wu, Zhimiao Xiong, Yan Zhou, Zhaoyang Ye*, Wen-Song Tan*. Mesenchymal Stem Cells Reshape and Provoke Proliferation of Articular Chondrocytes by Paracrine Secretion. Scientific Reports. 2016; 6: 32705.
24. Ludi Zhang, Yanjiao Shao, Lu Li, Feng Tian, Jin Cen, Xiaotao Chen, Dan Hu, Yan Zhou, Weifen Xie, Yunwen Zheng, Yuan Ji, Mingyao Liu, Dali Li*, Lijian Hui*. Efficient liver repopulation of transplanted hepatocyte prevents cirrhosis in a rat model of hereditary tyrosinemia type I. Scientific Reports. 2016; 6: 31460.
25. Xiao-Lei Shi, Yimeng Gao, Yupeng Yan, Hucheng Ma, Lulu Sun, Pengyu Huang, Xuan Ni, Ludi Zhang, Xin Zhao, Haozhen Ren, Dan Hu, Yan Zhou, Feng Tian, Yuan Ji, Xin Cheng, Guoyu Pan*, Yi-Tao Ding*, Lijian Hui*. Improved survival of porcine acute liver failure by a bioartificial liver device implanted with induced human functional hepatocytes.Cell Research. 2016; 26(2): 206-216.
26. Yingdi Zhao, Ke Tan, Yan Zhou, Zhaoyang Ye*, Wen-Song Tan. A combinatorial variation in surface chemistry and pore size of three-dimensional porous poly(?-caprolactone) scaffolds modulates the behaviors of mesenchymal stem cells. Materials Science and Engineering: C-Materials for Biological Applications. 2016; 1(59): 193-202.
27. Yaya Liu, Weiyan Zhang, Xiancun Deng, Hong Fai Poon, Xuping Liu, Wen-Song Tan, Yan Zhou, Li Fan*. Chinese hamster ovary cell performance enhanced by a rational divide-and conquer strategy for chemically defined medium development.Journal of Bioscience and Bioengineering. 2015; 120(6): 690-696.
28. Min Chen, Yi Zhang, Yan Zhou, Yan Zhang, Meidong Lang, Zhaoyang Ye*, Wensong Tan*. Pendant small functional groups on poly(?-caprolactone) substrate modulate adhesion, proliferation and differentiation of human mesenchymal stem cells. Colloids and Surfaces B Biointerfaces. 2015; 134: 322-331.
29. Houyong Luo, Maiqin Chen, Xiu Wang, Yang Mei, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Fabrication of centimeter-sized viable 3D tissue constructs endowed with microchannel conduits for improved tissue properties through assembling of cell-laden microbeads. Journal of Tissue Engineering and Regenerative Medicine. 2014; 8: 493-504.
30. Xiu Wang, Qingling Jiao, Songjie Zhang, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan. Perfusion culture induced template-assisted assembling of cell-laden microcarriers is a promising route for fabricating macrotissues. Biotechnology Journal. 2014; 9: 1425-1434.
31. Maiqin Chen, Min Zhou, Zhaoyang Ye*, Yan Zhou*, Wen-Song Tan*. Ectopic osteogenesis of macroscopic tissue constructs assembled from human mesenchymal stem cell-laden microcarriers through In vitro perfusion culture. PLoS ONE. 2014; 9(10): e109214.
32. Wei Wu, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells. The International Journal of Artificial Organs. 2011; 34(12): 1128-1136.
33. Maiqin Chen, Xiu Wang, Zhaoyang Ye, Yan Zhang, Yan Zhou*, Wen-Song Tan*. A modular approach to the engineering of centimeter-sized bone tissue constructs using human amniotic mesenchymal stem cells-laden microcarriers. Biomaterials. 2011; 32(30): 7532-7542.
34. Min Wang, Yan Zhou*, Wen-Song Tan. Clonal isolation and characterization of mesenchymal stem cells from human amnion, Biotechnology and Bioprocess Engineering. 2010; 15(6): 1047-1058.
35. Yang Mei, Qiang Tang, Zhaoyang Ye, Yan Zhou*, Wen-Song Tan. Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering, Journal of Biotechnology. 2010; 150(3): 438-446.
Corresponding address:
The State Key Laboratory of Bioreactor Engineering,
E-mail: zhouyan@ecust.edu.cn
Fax: +86-
Tel: +86-
