针对微生物及其它多个细胞系统的复杂性，开发各种代谢分析方法以理解细胞代谢网络的调控特性，获得细胞生长生产中的基本规律，为有用物质生产菌的代谢工程改造提供可靠的技术平台以及深入研究的新思路。具体研究包括利用代谢物同位体信息进行代谢流量组分析、结合多层面信息的微生物过程系统生物学方法开发、抗生素生产菌、生物柴油生产菌的代谢研究和工程改造等。

Leifang Lu, Liujing Wei, Kun Zhu, Dongzhi Wei, Qiang Hua*. Combining Metabolic Engineering and Adaptive Evolution to Enhance the Production of Dihydroxyacetone from Glycerol by Gluconobacter oxydans in a Low-cost Way. Bioresource Technology (Accepted).

Zhijie Liu, Xinran Wang, Qingsheng Qi, Qiang Hua*. Quantification and Analysis of Metabolic Characteristics of Aerobic Succinate-Producing Escherichia coli under Different Aeration Conditions. Process Biochemistry (Accepted).

Na Ma, Liujing Wei, Yuxiang Fan, Qiang Hua*. Heterologous expression and characterization of soluble recombinant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Actinosynnema pretiosum ssp. auranticum ATCC31565 through co-expression with Chaperones in Escherichia coli. Protein Expression and Purification, 82(2):263-269, 2012.

柳志杰, 周利, 花强*. 基于代谢流量分布信息理解大肠杆菌中异柠檬酸裂解酶调节因子的代谢调控作用. 《生物工程学报》, 28(5): 565−576, 2012.

张敏华, 韦柳静, 祝坤, 花强*. 氧化葡萄糖酸杆菌突变菌GDHK的葡萄糖培养基优化及催化研究. 高校化工学报（已录用）.

花强 等. 实验室适应性进化的研究进展. 《2010工业生物技术发展报告》, 科学出版社 2010.

花强 等. 代谢流量比率分析及其在代谢工程中的应用. 生物工程学报, 2009,25(9):1303-1311.

Hua, Q. et al. Metabolic characterization of E. coli adapted to growth on lactate. Appl Environ Microbiol, 73: 4639-4647, 2007.

Hua, Q. et al. Metabolic analysis of adaptive evolution for in silico designed lactate-producing strains. Biotechnol Bioeng, 95:992-1002, 2006.

Hua, Q. et al. Analysis of gene expression in E. coli in response to changes of growth-limiting nutrient in chemostat cultures. Appl Environ Microbiol, 70: 2354-2366, 2004.

Yang, C. et al. Analysis of E. coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to alter dilution rates and phosphoenolpyruvate carboxykinase knockout. Biotechnol Bioeng, 84:129-144, 2003.

Hua, Q. et al. Responses of the central metabolism in E. coli to phosphoglucose isomerase and glucose-6-phosphate dehydrogenase knockouts. J Bacteriol, 185: 7053-7067, 2003.