翁杰敏 生命医学研究所副所长
生命科学学院??????


导航
个人资料
研究方向
开授课程
科研项目
学术成果
荣誉及奖励







个人资料
部门： 生命科学学院
毕业院校：
学位：
学历：
邮编：
联系电话： **
传真： **
电子邮箱： jmweng@bio.ecnu.edu.cn
办公地址： 实验B楼512
通讯地址： 实验B楼512，东川路500号

教育经历

工作经历

个人简介

社会兼职
Member of Endocrine Society
Member of Microbiology Society


研究方向
表观遗传学‚细胞核激素受体‚干细胞和肿瘤
随着人类基因组测序的完成‚表观遗传学已成为引领生物学发展的重要前沿领域。表观遗传学主要研究细胞在增殖、分化、发育过程中的基因表达谱式建立及其维持的机制‚进而阐明基因表达谱式如何决定和维持细胞的表型特性。由于在增殖、分化、发育过程中基因组序列并不发生变化‚表观遗传学也定义为研究在基因序列不改变的情况下细胞所发生的可以遗传的表型变异机制。我们实验室主要研究： 1）表观遗传学的基本分子机制‚如组蛋白乙酰化和去乙酰化酶‚组蛋白甲基化和去甲基化酶的作用机制和功能；2）组蛋白修饰密码的识别和功能解读；3）干细胞和肿瘤的表观遗传调控。此外‚我们还研究细胞核激素受体基因表达调控的分子机制以及在肿瘤发生中的作用。


开授课程


科研项目


学术成果
1. Chen‚ H.B.‚ Wong‚ J.‚ Jiang‚ K. and Bao‚ J.S. 1990. A new method for the synthesis of a structural gene. Nucleic Acid Research 18‚ 871-878.
2. Wong‚ J.‚ Liu‚ F.‚ and Bateman‚ E. 1992. Cloning and expression of the Acathamoeba castellanii gene encoding transcription factor TFIID. Gene 117‚ 91-97.
3. Wong‚ J.‚ Liu‚ F.‚ and Bateman. E. 1992. Isolation of genomic DNA encoding transcription factor TFIID from Acanthamoeba castellanii:: characterization of the promoter. Nucleic Acids Res. 20‚ 4817-4826.
4. Radebaugh‚ C.A.‚ Matthews‚ J. L.‚ Geiss‚ G. K.‚ Liu‚ F.‚ Wong‚ J.‚ Bateman‚ E.‚ Camier‚ S.‚ Sentenac‚ A. and Paule‚ M. R. 1994. TATA-box binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I‚ II‚ and III transcription factors. Mol. Cell. Biol. 14‚ 597-605.
5. Wong‚ J. and Bateman‚ E. 1994. TBP-DNA interactions in the minor groove discriminate between A:T and T:A base pairs. Nucleic Acids Res. 22‚ 1890-1896.
6. Wong‚ J. and Bateman‚ E. 1994. Cloning of the cDNA encoding an Acanthamoeba castellanii PDI-like protein. Gene. 150‚ 175-179.
7. Ranjan‚ M.‚ Wong‚ J. and Shi‚ Y-B. 1994. Transcriptional repression of Xenopus TRbeta gene is mediated by a thyroid hormone response element located near the start site. J. Biol. Chem. 269‚ 24699-24705.
8. Wong‚ J.‚ Shi‚ Y-B. and Wolffe‚ A.P. 1995. A role of nucleosome assembly in the transcriptional regulation of Xenopus TRA gene by thyroid hormone receptor. Gene & Development. 9‚ 2696-2711.
9. Wong‚ J. and Shi‚ Y-B. 1995. Coordinated regulation of and transcriptional activation by Xenopus thyroid hormone and retinoid X receptors. J. Biol. Chem. 270‚ 18479-18483.
10. Waibao Huang‚ Wong‚ J. and Bateman‚ E. 1996. TATA elements direct bi-directional transcription by RNA polymerase II and III. Nucleic Acid Res. 24‚ 1158-1163..
11.Shi‚ Y.-B.‚ M.A. Stolow‚ M. Puzianowska-Kuznicka‚ and Wong‚ J. 1996. Les genes de la metamorphose. La Recherche 286: 58-64.
12.Shi‚ Y.-B.‚Wong‚ J.‚ M. Puzianowska-Kuznicka and M.A. Stolow. 1996. Tadpole Competence and Tissue-Specific Temporal Regulation of Amphibian Metamorphosis: Roles of Thyroid Hormone and Its Receptors. Bioessays 18(5): 391-399.
13. Puzianowska-Kuznicki‚ M.‚ Wong‚ J. and Shi‚ Y-B. 1996. Functional characterization of a mutant thyroid hormone receptor in Xenopus Laevis. J. Biol. Chem 270‚ 33394-33403
14. Wong‚ J.‚ Shi‚ Y-B.‚ and Wolffe‚ A.P. 1997. Determinants of chromatin disruption and transcriptional regulation instigated by the thyroid hormone receptor: hormone-regulated chromatin disruption is not sufficient for transcriptional activation. EMBO J. 16(11): 3158-3171.
15. Wong‚ J.‚ Li‚ Q.‚ Levi‚ B-Z.‚ Shi‚ Y-B.‚ and Wolffe‚ A.P. 1997. Structural and functional features of a specific nucleosome containing a recognition element for the thyroid hormone receptor. EMBO J. 16(23): 7130-7145.
16. Wong‚ J.‚ Patterton‚ D.‚ Imhof‚ A.‚ Guschin‚ D.‚ Shi‚ Y-B.‚ and Wolffe AP. 1998. Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase. EMBO J. 17(2): 520-534.
17. Minucci‚ S.‚ Wong‚ J.‚ Blanco‚ J.C.‚ Shi‚ Y-B.‚ Wolffe‚ A.P.‚ and Ozato K. 1998. Retinoid receptor-induced alteration of the chromatin assembled on a ligand-responsive promoter in Xenopus oocytes. Mol Endocrinol. 12(3):315-324.
18. Wolffe‚ A.P.‚ Wong‚ J.‚ and Pruss‚ D. 1997. Activators and repressors: making use of chromatin to regulate transcription. Genes Cells 2(5):291-302.
19. Wolffe‚ A.P.‚ Wong‚ J.‚ Li‚ Q.‚ Levi‚ B.Z.‚ and Shi Y-B. 1997. Three steps in the regulation of transcription by the thyroid hormone receptor: establishment of a repressive chromatin structure‚ disruption of chromatin and transcriptional activation. Biochem Soc Trans 25(2):612-615.
20. Wong J.‚ Liang‚ V.C.‚ Sachs‚ L.M.‚ and Shi‚ Y.B. 1998. Transcription from the thyroid hormone-dependent promoter of the Xenopus laevis thyroid hormone receptor betaA gene requires a novel upstream element and the initiator‚ but not a TATA Box. J Biol Chem 273:14186-93.
21. Xue‚ Y.‚ Wong‚ J.‚ Moreno‚ G.T.‚ Young‚ M.A.‚ Cote‚ J.‚ and Wang‚ W. 1998. NURD‚ a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities. Molecular Cell 2: 851-861.
22. Lanz‚ R. B.‚ Mckenna‚ N.J.‚ Onate‚ S.A.‚ Albrecht‚ U.‚ Wong‚ J.‚ Tsai‚ S.Y.‚ Tsai‚ M-J. and O’Malley B.W. 1999. A steroid receptor coactivator‚ SRA‚ functions as an RNA and is present in an SRC-1 complex. Cell 97: 17-27.
23. Liu‚ Z.‚ Wong‚ J.‚ Tsai‚ S.Y.‚ Tsai‚ M-J and O’Malley B.W. 1999. Steroid receptor coactivator-1 (SRC-1) enhances ligand-dependent and receptor-dependent cell free transcription of chromatin. PNAS 96: 9485-9490.
24. Li‚ J.‚ O’Malley B.W and Wong J. 2000. p300 requires its HAT activity and SRC-1 interaction motif to facilitate TR activation in chromatin. Mol. Cell. Biol. 20: 2031-2042.
25. Li‚ J‚. Wang‚ J; Wang‚ J; Nawaz‚ Z; Liu‚ J.‚ Jun‚ Q.‚ and Wong‚ J. 2000. Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3. EMBO J. 19(16): 4342-4350.
26. Wang‚ H.‚ Huang‚ Z.‚ Xia‚ L.‚ Feng‚ Q.‚ Erdjument-Bromage‚ H.‚ Strahl‚ BD.‚ Briggs‚ SD.‚ Allis‚ CD.‚ Wong‚ J.‚ Tempst‚ p and Zhang‚ Y. 2001. Methylation of Histone H4 at Arginine 3 Facilitates Transcriptional Activation by Nuclear Hormone Receptor. Science 293: 853-857.
27. Liu‚ Z.‚ Wong‚ J.‚ Tsai‚ S.Y.‚ Tsai‚ M-J and O’Malley B.W. 2001. Sequential recruitment of steroid receptor coactivator-1 (SRC-1) and p300 enhances progesterone receptor-dependent initiation and reinitiation of transcription from chromatin. PNAS 98: 12426-12431.
28. Huang‚ Z.‚ Li‚ J. and Wong‚ J. 2002. Androgen receptor possesses an intrinsic-hormone-independent trans-activation activity. Molecular Endocrinology. 16: 924-937.
29. Liu Z‚ Auboeuf D‚ Wong J‚ Chen JD‚ Tsai SY‚ Tsai MJ‚ O´Malley BW. 2002. Coactivator/corepressor ratios modulate PR-mediated transcription by the selective receptor modulator RU486. Proc Natl Acad Sci U S A. 99(12):7940-4.
30. Li‚ J; Lin‚ Q; Wade‚ P.‚ Wang‚ W and Wong‚ J. 2002. Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression. Genes & Development 16: 687-692.
31. Wu‚ R.C.‚ Qin‚ J.‚ Hashimoto‚ Y.‚ Wong‚ J.‚ Xu‚ J.‚ Tsai‚ S.Y.‚ Tsai‚ M.J‚ and O´Malley B.W. 2002. Regulation of SRC-3 (pCIP/ACTR/AIB-1/RAC-3/TRAM-1) Coactivator Activity by IkappaB Kinase. Mol Cell Biol 22(10):3549-61.
32. Li‚ J.‚ Lin‚ Q.‚ Yoon‚ H-G.‚ Huang‚ Z.‚ Strahl‚ B.D.‚ Allis‚ C.D.‚ and Wong‚ J. 2002. The involvement of multiple histone methylation in transcriptional regulation by thyroid hormone receptor. Mol Cell Biol 22 (16): 5688-5697.
33. Suh YA‚ Lee HY‚ Virmani A‚ Wong J‚ Mann KK‚ Miller WH Jr‚ Gazdar A‚ Kurie JM. 2002. Loss of retinoic acid receptor beta gene expression is linked to aberrant histone H3 acetylation in lung cancer cell lines. Cancer Res. 2002 Jul 15;62(14):3945-9.
34. Kraus WL and Wong J. 2002. Nuclear Receptor-Dependent transcription from Chromatin: Its All About Enzymes? Eur J Biochem 269:1-9.
35. Wong J. 2002. Transcriptional regulation by thyroid hormone receptor in chromatin. Thyroid hormone receptors: Methods in Molecular Biology edited by Aria Baniahmad‚ p177-194.
36. Osborne CK‚ Bardou V‚ Hopp TA‚ Chamness GC‚ Hilsenbeck SG‚ Fuqua SA‚ Wong J‚ Schiff R‚ Allred DC and Clark GM. 2003. Overexpression of the Estrogen Receptor Coactivator AIB1 (SRC3) and the HER-2/neu Oncogene are Both Necessary for the Tamoxifen-Resistance in ER-Positive Primary Breast Cancers. J Natl Cancer Insti. 95(5):353-61.
37. Cheskis BJ‚ McKenna NJ‚ Wong CW‚ Wong J‚ Komm B‚ Lyttle CR‚ O´Malley BW. 2003. Hierarchical affinities and a bipartite interaction model for estrogen receptor isoforms and full length steroid receptor coactivator (SRC/p160) family members. J Biol Chem. 278(15):13271-7.
38. Yoon HG‚ Chan DW‚ Huang ZQ‚ Li J‚ Fondell JD‚ Qin J and Wong J. 2003. Purification and Functional Characterization of the Human N-CoR Complex: the Roles of HDAC3 and two WD-40 Repeat Subunits TBL1 and TBLR1. EMBO J 22(6):1336-1346.
39. Lee KC^#‚ Li J^#‚ Cole PA‚ Wong J* and Kraus L*. 2003. Transcriptional activation by thyroid hormone receptor involves chromatin remodeling‚ histone acetylation‚ and cooperative stimulation by p300 and SRC coactivators. Molecular Endocrinology‚ 17(5):908-22. (#equal contribution and co-corresponding authors.)
40. Huang ZQ‚ Li J‚ Sachs LM‚ Cole PA and Wong J. 2003. A Role for Cofactor-Cofactor and Cofactor-Histone Interaction in Targeting of CBP/p300‚ SWI/SNF and Mediator for transcription. EMBO J 22(9):2146-2155.
41. Li X‚ Wong J‚ Tsai S‚ Tsai M and O’malley BW. 2003. Progesterone and Glucocorticoid Receptors Recruite distinct Coactivator Complexes and Promote Distinct Patterns of Local Chromatin Modification. Mol Cell Biol. 23(11):3763-73.
42. Yoon HG‚ Chan DW‚ Reynolds AB‚ Qin J and Wong J. 2003. Kaiso targets the N-CoR corepressor complex for DNA methylation mediated repression. Molecular Cell 12‚ 723-734.
43. Xin H‚ Yoon HG‚ Singh PB‚ Wong J‚ Qin J. 2004. Components of a pathway maintaining histone modification and HP1 binding at the pericentric heterochromatin in mammalian cells. J Biol Chem. 279(10):9539-46.
44. Paul R. Thompson‚ Dongxia Wang‚ Ling Wang‚ Marcella Fulco‚ Natalia Pediconi‚ DianZheng Zhang‚ Woojin An‚ Qingyuan Ge‚ Robert G. Roeder‚ Wong J‚ Massimo Levrero‚ Vittorio Sartorelli‚ Robert J. Cotter‚ Philip A. Cole. 2004. Regulation of the p300 HAT Domain via a Novel Activation Loop. Nature Structural and Molecular Biology. 11(4): 308-15.
45. Wu RC‚ Qin J‚ Yi P‚ Wong J‚ Tsai SY‚ Tsai MJ‚ O´Malley BW. 2004. Selective Phosphorylations of the SRC-3/AIB1 Coactivator Integrate Genomic Reponses to Multiple Cellular Signaling Pathways. Molecular Cell.15(6):937-49.
46. Stewart MD and Wong J. 2004. Curr. Opin. Endocrinol. Diabetes 11: 218-225.
47. Yoon HG‚ Choi Y‚ Cole PA and Wong J. 2005 Reading and Function of a histone code involved in targeting corepressor complexes for repression. Mol Cell Biol;25(1):324-335.
48. Stewart MD‚ Li J and Wong J. 2005 Relationship between H3 lysine 9 methylation‚ transcription repression and heterochromatin protein 1 recruitment. Mol Cell Biol;25(7):2525-38.
49. Zhang D‚ Yoon HG and Wong J. 2005. JMJD2A is a novel N-CoR-interacting protein and is involved in repression of the human transcription factor achaete scute-like homologue 2 (ASCL2/Hash2). Mol Cell Biol;25(15):6404-14.
50. Burd CJ‚ Petre CE‚ Morey LM‚ Wang Y‚ Revelo MP‚ Haiman CA‚ Lu S‚ Fenoglio-Preiser CM‚ Li J‚ Knudsen ES‚ Wong J and Knudsen KE. 2006 Cyclin D1b variant influences prostate cancer growth through aberrant androgen receptor regulation. Proc Natl Acad Sci U S A; 103:2190-5.
51. Yi P‚ Wu RC‚ Sandquist J‚ Wong J‚ Tsai SY‚ Tsai MJ‚ Means AR‚ O´Malley BW. 2005. Peptidyl-prolyl isomerase 1 (Pin1) serves as a coactivator of steroid receptor by regulating the activity of phosphorylated steroid receptor coactivator 3 (SRC-3/AIB1). Mol Cell Biol.25(21):9687-99.
52. Yoon‚ H. G.‚ and Wong J. 2006. The corepressors silencing mediator of retinoid and thyroid hormone receptor and nuclear receptor corepressor are involved in agonist- and antagonist-regulated transcription by androgen receptor. Mol Endocrinol 20:1048-60.
53. Jiang S‚ Meyer R‚ Kang K‚ Osborne CK‚ Wong J‚ Oesterreich S. 2006. Scaffold attachment factor SAFB1 suppresses estrogen receptor alpha-mediated transcription in part via interaction with nuclear receptor corepressor. Mol Endocrinol. 20(2):311-20.
54. Stewart D‚ Tomita A‚ Shi YB‚ Wong J. 2006. Chromatin immunoprecipitation for studying transcriptional regulation in Xenopus oocytes and tadpoles. Methods Mol Biol. 322:165-81. Review.
55. Klose‚ R. Yamane JK‚ Bae Y‚ Zhang D‚ Erdjument-Bromage H‚ Tempst P‚ Wong J and Zhang Y. 2006. The transcriptional repressor JHDM3A demethylates trimethyl histone H3 lysine 9 and lysine 36. Nature 442:312-316.
56. Li‚ J.‚ Fu J‚ Toumazou C‚ Yoon HG‚ and Wong J. 2006. A role of the amino-terminal (N) and carboxyl-terminal (C) interaction in binding of androgen receptor to chromatin. Mol Endocrinol 20:776-85.
57. Qiu J‚ Wong J‚ Tweardy DJ‚ Dong S. Decreased intranuclear mobility of acute myeloid leukemia 1-containing fusion proteins is accompanied by reduced mobility and compartmentalization of core binding factor beta. Oncogene. 2006 Jun 29;25(28):3982-93.
58. Yamane‚ K.‚ Toumazou C‚ Tsukada YI‚ Erdjument-Bromage H‚ Tempst P‚ Wong J and Zhang Y. 2006. JHDM2A‚ a JmjC-Containing H3K9 Demethylase‚ Facilitates Transcription Activation by Androgen Receptor. Cell 125:483-95.
59. Feng‚ Q.‚ P. Yi‚ J. Wong‚ and W. O´Malley B. 2006. Signaling within a coactivator complex: Methylation of SRC-3/AIB1 is a molecular switch for complex disassembly. Mol Cell Biol. 26:7846-57.
60. Stewart‚ M. D.‚ J. Sommerville‚ and J. Wong. 2006. Dynamic regulation of histone modifications in Xenopus oocytes through histone exchange. Mol Cell Biol 26:6890-901.
61. Grégoire‚ S.‚ Lin Xiao‚ Jianyun Nie‚ Xiaohong Zhang‚ Minghong Xu‚ Jiarong Li‚ J. Wong‚ Edward Seto‚ and Xiang-Jiao Yang. 2007. Histone deacetylase 3 interacts with and deacetylates MEF2 transcription factors. Mol Cell Biol 27:1280-95.
62. Fu‚ J.‚ Yoon‚ HG.‚ Qin‚ J. and Wong J. Regulation of P-TEFb Elongation Complex Activity by CDK9 Acetylation. Mol Cell Biol. 2007 Apr 23; [Epub ahead of print]
63. Karagianni P and Wong J. 2007. HDAC3: taking the SMRT-N-CoRrect road to repression. Oncogene 26(37):5439-49.
64. Zhang D‚ Cho E‚ and Wong J. 2007. A critical role for the co-repressor N-CoR in erythroid differentiation and heme synthesis. Cell Research 17:804-814.
65. Karagianni P‚ Amazit L‚ Qin J‚ Wong J. 2008. ICBP90‚ a novel methyl K9 H3 binding protein linking protein ubiquitination with heterochromatin formation. Mol Cell Biol 28(2):705-17
66. Ishii S‚ Kurasawa Y‚ Wong J‚ Yu-Lee LY. 2008. Histone deacetylase 3 localizes to the mitotic spindle and is required for kinetochore-microtubule attachment. PNAS 105(11):4179-84.
67. Liang J‚ Wan M‚ Zhang Y‚ Gu P‚ Xin H‚ Jung SY‚ Qin J‚ Wong J‚ Cooney AJ‚ Liu D‚ and Songyang Z. Nanog and Oct4 associate with unique transcriptional repression complexes in embryonic stem cells. Nature Cell Biology. In press.


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