郑雪莲
职称：副教授
邮箱：zhengxl@uestc.edu.cn
所在系别：生物技术系
研究领域：植物基因组工程



教育背景
1996年9月—2000年6月，西南师范大学（现西南大学），生命科学与技术学院，生物教育专业，本科
2000年9月—2007年6月，西南大学，生物技术中心，作物遗传育种专业，博士
工作履历
2011年7月—今，电子科技大学，生命科学与技术学院，副教授
2007年7月—2011年6月，电子科技大学，生命科学与技术学院，讲师
2003年7月－2004年9月，美国康涅狄格大学，植物系，访问****
科学研究
研究概况
致力于基因组编辑技术与合成生物学领域的研究和应用，基于CRISPR-Cas平台开展水稻、马铃薯、丹参等植物的基因功能研究及应用实践。主要参与开发了two-RVD TALEN快速组装文库，CRISPR-Cas9 与CRISPR-Cas12a基因定向编辑系统，SSCP快速基因分型技术，以及高效单一转录单元STU-CRISPR 1.0，2.0及3.0基因编辑系统。同时，将植物基因组编辑技术应用于水稻淀粉合成的分子机制研究、高寒粳稻品种的产量品质改良及丹参次生代谢产物的合成调控中。先后承担国家科技重大专项3项，国家自然科学基金项目7项，以及多项省部级科研项目。在Nature Biotechnology, Nature Plants, Genome Biology, Molecular Plant, Plant Biotechnology Journal, Frontiers in Plant Science, Plant Physiology, Plant Cell Reports等国内外重要期刊上发表论文30余篇；申请发明专利近30项，授权发明专利6项。
代表性成果
主要科研项目
(1)国家自然科学基金面上项目，**，基于水稻支链淀粉生物合成途径多基因定向聚合突变体库的淀粉合成机制研究。2021/01-2024/12，在研，主持
(2)国家转基因重大专项子课题，2019ZX**-001-002，基于大数据挖掘的水稻CRISPR-Cas精准基因组编辑新系统构建及应用，2019/01-2020/12，在研，主持
(3)国家自然科学基金面上项目，**，基于合成生物学的丹参酚酸类成分基因尺度代谢网络调控机制研究，2019/01-2022/12，在研，参加
(4)国家自然科学地区基金项目，**，基于CRISPR/Cas基因编辑系统的高海拔冷凉粳稻多基因定向聚合育种体系构建及高产优质材料创制，2020/01-2023/12，在研，参加。
(5)国家转基因重大专项子课题，2018ZX**-003，落叶松基因组编辑及基因工程新技术构建应用，2018/01-2020/12，在研，参加
(6)四川省科技计划国际合作项目，2018HH0112，基于基因组编辑技术快速创制水稻高产优质新材料/新品种，2018/04-2019/12，已结题，参加
(7)四川省科技计划青年基金项目，2017JQ0005，水稻基因组增强子高效挖掘及定向修饰功能研究，2017/03-2020/03,50万元，已结题，参加
(8)国家自然科学基金面上项目，**，马铃薯TALEN基因组定向突变技术体系的建立及在淀粉合成机理研究上的应用，2014/01-2017/12，已结题，主持
(9)国家转基因重大专项子课题，2014ZX**B-002，高效TALEN介导的小麦基因组定向编辑技术体系的优化，2014/01-2016/12，已结题，主持
(10)国家自然科学基金青年基金，**，甘薯组织特异启动子的筛选与功能鉴定，2013/01-2016/12，已结题，主持
主要代表性论文：
(1)Qiurong Ren , Simon Sretenovic , Shishi Liu , Xu Tang , Lan Huang , Yao He , Li Liu , Yachong Guo , Zhaohui Zhong , Guanqing Liu , Yanhao Cheng , Xuelian Zheng , Changtian Pan , Desuo Yin , Yingxiao Zhang , Wan-Feng Li , Liwang Qi , Chenghao Li , Yong Zhang, Yiping Qi. PAM-less plant genome editing using a CRISPR-SpRY toolbox. Nature Plant. 2020,
(2)Xuelian Zheng, Caiyan Qi, Lijia Yang, Quan Quan, Binglin Liu, Zhaohui Zhong, Xu Tang, Tingting Fan, Jianping Zhou and Yong Zhang. The improvement of CRISPR-Cas9 system with ubiquitin-associated domain fusion for efficient plant genome editing. Front. Plant Sci, 2020, 11:621. doi:10.3389/fpls.2020.00621.
(3)Xu Tang, Simon Sretenovic, Qiurong Ren, Xinyu Jia, Mengke Li, Tingting Fan, Desuo Yin, Shuyue Xiang, Yachong Guo, Li Liu, Xuelian Zheng, Yiping Qi, Yong Zhang. Plant prime editors enable precise gene editing in rice cells. Molecular Plant. 2020, 13:667-670.
(4)Meiling Ming, Qiurong Ren, Changtian Pan, Yao He, Yingxiao Zhang, Shishi Liu, Zhaohui Zhong, Jiaheng Wang, Aimee A. Malzahn1, Jun Wu, Xuelian Zheng, Yong Zhang, Yiping Qi. CRISPR–Cas12b enables efficient plant genome engineering. Nature Plants. 2020, 6:202–208
(5)Zhong, Zhaohui#; Liu, Shishi#; Liu, Xiaopei; Liu, Binglin; Tang, Xu; Ren, Qiurong; Zhou, Jianping; Zheng, Xuelian; Qi, Yiping*; Zhang, Yong*; Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing, Rice, 2020, 13: 8.
(6)Tang, Xu#; Ren, Qiurong#; Yang, Lijia; Bao, Yu; Zhong, Zhaohui; He, Yao; Liu, Shishi; Qi, Caiyan; Liu, Binglin; Wang, Yan; Sretenovic, Simon; Zhang, Yingxiao; Zheng, Xuelian; Zhang, Tao*; Qi, Yiping*; Zhang, Yong*; Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing , Plant Biotechnology Journal, 2019, 17(7): 1431-1445.
(7)Malzahn AA, Tang X, Lee K, Ren Q, Sretenovic S, Zhang Y, Chen H, Kang M, Bao Y, Zheng X, Deng K, Zhang T, Salcedo V, Wang K, Zhang Y, Qi Y. Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis. BMC Biology, 2019, 17(1):9.
(8)Qiurong Ren, Zhaohui Zhong, Yan Wang, Qi You, Qian Li, Mingzhu Yuan, Yao He,Caiyan Qi, Xu Tang, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang. Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front. Plant Sci, 2019, 10:1173.
(9)WANG Bo, ZHONG Zhaohui, ZHANG Huanhuan, WANG Xia, LIU Binglin, YANG Lijia, HAN Xiangyan, YU Deshui, ZHENG Xuelian, WANG Chunguo, SONG Wenqin, CHEN Chengbin, ZHANG Yong. Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice. Rice Science, 2019, 26(2): 98-108.
(10)Zhou J, Xin X, He Y, Chen H, Li Q, Tang X, Zhong Z, Deng K, Zheng X, Akher SA, Cai G, Qi Y, Zhang Y. Multiplex QTL editing of grain-related genes improves yield in elite rice varieties. Plant Cell Reports, 2019, 38: 475–485.
(11)Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang. Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9. 2019, Plant Genome Editing with CRISPR Systems: Methods and Protocols, Methods in Molecular Biology, vol. 1917
(12)Zheng X, Yang L, Li Q, Ji L, Tang A, Zang L, Deng K, Zhou J, Zhang Y, MIGS as a Simple and Efficient Method for Gene Silencing in Rice, Front. Plant Sci, 2018, 9:662.
(13)Tang, Xu; Liu, Guanqing; Zhou, Jianping; Ren, Qiurong; You, Qi; Tian, Li; Xin, Xuhui; Zhong, Zhaohui; Liu, Binglin; Zheng, Xuelian; Zhang, Dengwei; Malzahn, Aimee; Gong, Zhiyun; Qi, Yiping; Zhang, Tao; Zhang, Yong. A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice. Genome Biology. 2018, 19:84.
(14)Zhong, Zhaohui; Zhang, Yingxiao; You, Qi; Tang, Xu; Ren, Qiurong; Liu, Shishi; Yang, Lijia; Wang, Yan; Liu, Xiaopei; Liu, Binglin; Zhang, Tao; Zheng, Xuelian; Le, Ysa; Zhang, Yong; Qi, Yiping, Plant genome editing using FnCpf1 and LbCpf1 nucleases at redefined and altered PAM sites, Molecular Plant, 2018, 7:999-1002.
(15)周建平,吉琳艺,何瑶,郑雪莲,罗樊,蔡光泽,张勇.利用CRISPR-Cas9系统高效创制水稻品系“17318”的OsGW2、OsGN1a基因定向突变体.西昌学院学报(自然科学版), 2018, 32:1-5.
(16)Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng XL, Voytas DF, ZhongZH, Chen YY, Ren QR, Li Q, Kirkland ER, Zhang Y, Qi Y, A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants, Nature Plants, 2017, 3(7): 17018
(17)Zhou JP#, Deng KJ#, Cheng Y, Zhong ZH, Tian L, Tang X, Tang AT, Zheng XL, Zhang T, Qi YP, Zhang Y*, CRISPR-Cas9 Based Genome Editing Reveals New Insights into MicroRNA Function and Regulation in Rice, Frontiers in Plant Science, 2017, 8:1598
(18)Liu Y, Sun G, Zhong ZH, Ji LY, Zhang Y, Zhou JP, Zheng XL*, Deng KJ*. Overexpression of AtEDT1 promotes root elongation and affects medicinal secondary metabolite biosynthesis in roots of transgenic Salvia miltiorrhiza. Protoplasma, 2017, 254(4):1617-1625
(19)Wei T, Deng K, Zhang Q, Gao Y,Liu Y, Yang M, Zhang L, Zheng X, Wang C, Liu Z, Chen C* and Zhang Y*. Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza. Front Plant Sci. 2017, 8: 52.
(20)刘玉; 孙耿; 仲昭晖; 郑雪莲(*); 邓科君(*), 烟草中过表达丹参SAMDC基因增强其抗旱性（英文）, 农业生物技术学报, 2017.3, (05)：729~738.
(21)Tang Xu*, Zheng Xuelian*, Qi Yiping, Zhang Dengwei, Cheng Yan, Tang Aiting, Daniel F. Voytas, Zhang Yong, A single transcript CRISPR-Cas9 system for efficient genome editing in plants, Molecular Plant, 2016, 9(7): 1088~1091
(22)Zheng Xuelian, Yang Shixin, Zhang Dengwei, Zhong Zhaohui, Tang Xu, Deng Kejun, Zhou Jianping, Qi Yiping, Zhang Yong, Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism, Plant Cell Reports, 2016, 35(7): 1545~1554
(23)Zheng Xuelian, Zhou Jianping, Zang Lili, Tang Aiting, Liu Dongqing, Deng Kejun, Zhang Yong, Genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient. Genetics and Molecular Research, 2016, 15(2):
(24)Deng K，Zang L，Lan X，Zhong Z，Xiong B，Zhang Y，Zheng X(*). Antioxidant components from Cape Gooseberry. Journal of Food Processing and Preservation. 2016 40(5): 893-898.
(25)Deng K，Lan X，Sun G, Ji L, Zheng X(*). Determination of sulfonamide residues in chicken liver using high-performance liquid chromatography. Food Analytical Methods. 2016 9,(12): 3337–3344
(26)Zheng Xuelian, Li Qian, Liu Dongqing, Zang Lili, Zhang Kaiyue, Deng Kejun, Yang Shixin, Xie Zhengyang, Tang Xu, Qi Yiping, Zhang Yong, Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum. Plant Cell Reports, 2015, 34(11): 1873~1884
(27)Levi G. Lowder, Zhang Dengwei, Nicholas J. Baltes, Joseph W. Paul III, Tang Xu, Zheng Xuelian, Daniel F. Voytas, Tzung-Fu Hsieh, Zhang Yong, Qi Yiping, A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation, Plant Physiology, 2015, 169: 971~985
(28)Liu Yu, Yang Shixin, Cheng Yan, Liu Dongqing, Zhang Yong, Deng Kejun, Zheng Xuelian*, Production of Herbicide-Resistant Medicinal Plant Salvia miltiorrhiza Transformed with the Bar Gene. Appl Biochem Biotechnol, 2015, 177: 1456~1465
(29)Shan Qiwei, Wang Yanpeng, Chen Kunling, Liang Zhen, Li Jun, Zhang Yi, Zhang Kang, Liu Jinxing, Voytas Daniel F, Zheng Xuelian, Zhang Yong, Gao Caixia, Molecular Plant, 2013, 6(4): 1365~1368
(30)Zhang Mi*, Zheng Xuelian*, Song Shuiqing, Zeng Qiwei, Hou Lei, Li Demou, Zhao Juan, Wei Yuan, Li Xianbi, Luo Ming, Xiao Yuehua, Luo Xiaoying, Zhang Jinfa, Xiang Chengbin, Pei Yan, Nature Biotechnology, 2011, 29(5): 453~45
授权发明专利：
(1)张勇，郑雪莲，邓科君，唐旭，章登位，一种CRISPR/Cas9单一转录单元定向修饰骨架载体及其应用，2015-7，中国，ZL1.1
(2)邓科君、郑雪莲、张勇，一种基于液体培养基的白芨种苗生产方法，2015-12，中国，ZL7.2
(3)郑雪莲，张勇，邓科君，臧黎黎，一种基于人工tasiRNA途径的水稻RNAi骨架载体构建及应用，2016-2，中国，ZL5.5
(4)郑雪莲，张勇，邓科君，仲昭辉，一种用于TALEN高效构建的双RVD单元模块库及TALEN构建方法，2016-5，中国，ZL8.0
(5)周建平，张勇，郑雪莲，唐旭，程燕，邓科君，一种水稻miRNA定向敲除的方法，2016-9，中国，ZL3.6
(6)汤丽霞、李思扬、张勇、郑雪莲、刘炳麟、邹佳佳，高催化活性的PET水解酶突变体，2017-10，中国，ZL8.4
教育教学
本科生课程
《分子生物学》《植物组织培养技术》《合成生物学与iGEM》《定制生命：基因组合成生物学原理及实践》《解码基因》等
研究生课程
《基因组工程与合成生物学》等
招生专业
博士：083100生物医学工程（06生物化学与分子生物学）
硕士：071000生物学（01生物化学与分子生物学 04细胞生物学 06遗传学）
个人/团队主页

职称 副教授 邮箱 zhengxl@uestc.edu.cn
所在系别 生物技术系 研究领域 植物基因组工程
科学研究 招生专业 博士：083100生物医学工程（06生物化学与分子生物学）</br> 硕士：071000生物学（01生物化学与分子生物学 04细胞生物学 06遗传学）
联系方式 办公地址
团队联系方式 教育教学 1996年9月—2000年6月，西南师范大学（现西南大学），生命科学与技术学院，生物教育专业，本科</br>
2000年9月—2007年6月，西南大学，生物技术中心，作物遗传育种专业，博士

工作履历 2011年7月—今，电子科技大学，生命科学与技术学院，副教授</br>
2007年7月—2011年6月，电子科技大学，生命科学与技术学院，讲师</br>
2003年7月－2004年9月，美国康涅狄格大学，植物系，访问**** 研究概况 致力于基因组编辑技术与合成生物学领域的研究和应用，基于CRISPR-Cas平台开展水稻、马铃薯、丹参等植物的基因功能研究及应用实践。主要参与开发了two-RVD TALEN快速组装文库，CRISPR-Cas9 与CRISPR-Cas12a基因定向编辑系统，SSCP快速基因分型技术，以及高效单一转录单元STU-CRISPR 1.0，2.0及3.0基因编辑系统。同时，将植物基因组编辑技术应用于水稻淀粉合成的分子机制研究、高寒粳稻品种的产量品质改良及丹参次生代谢产物的合成调控中。先后承担国家科技重大专项3项，国家自然科学基金项目7项，以及多项省部级科研项目。在Nature Biotechnology, Nature Plants, Genome Biology, Molecular Plant, Plant Biotechnology Journal, Frontiers in Plant Science, Plant Physiology, Plant Cell Reports等国内外重要期刊上发表论文30余篇；申请发明专利近30项，授权发明专利6项。
代表性成果 主要科研项目</br>
(1)国家自然科学基金面上项目，**，基于水稻支链淀粉生物合成途径多基因定向聚合突变体库的淀粉合成机制研究。2021/01-2024/12，在研，主持</br>
(2)国家转基因重大专项子课题，2019ZX**-001-002，基于大数据挖掘的水稻CRISPR-Cas精准基因组编辑新系统构建及应用，2019/01-2020/12，在研，主持</br>
(3)国家自然科学基金面上项目，**，基于合成生物学的丹参酚酸类成分基因尺度代谢网络调控机制研究，2019/01-2022/12，在研，参加</br>
(4)国家自然科学地区基金项目，**，基于CRISPR/Cas基因编辑系统的高海拔冷凉粳稻多基因定向聚合育种体系构建及高产优质材料创制，2020/01-2023/12，在研，参加。</br>
(5)国家转基因重大专项子课题，2018ZX**-003，落叶松基因组编辑及基因工程新技术构建应用，2018/01-2020/12，在研，参加</br>
(6)四川省科技计划国际合作项目，2018HH0112，基于基因组编辑技术快速创制水稻高产优质新材料/新品种，2018/04-2019/12，已结题，参加</br>
(7)四川省科技计划青年基金项目，2017JQ0005，水稻基因组增强子高效挖掘及定向修饰功能研究，2017/03-2020/03,50万元，已结题，参加</br>
(8)国家自然科学基金面上项目，**，马铃薯TALEN基因组定向突变技术体系的建立及在淀粉合成机理研究上的应用，2014/01-2017/12，已结题，主持</br>
(9)国家转基因重大专项子课题，2014ZX**B-002，高效TALEN介导的小麦基因组定向编辑技术体系的优化，2014/01-2016/12，已结题，主持</br>
(10)国家自然科学基金青年基金，**，甘薯组织特异启动子的筛选与功能鉴定，2013/01-2016/12，已结题，主持</br>
主要代表性论文：</br>
(1)Qiurong Ren , Simon Sretenovic , Shishi Liu , Xu Tang , Lan Huang , Yao He , Li Liu , Yachong Guo , Zhaohui Zhong , Guanqing Liu , Yanhao Cheng , Xuelian Zheng , Changtian Pan , Desuo Yin , Yingxiao Zhang , Wan-Feng Li , Liwang Qi , Chenghao Li , Yong Zhang, Yiping Qi. PAM-less plant genome editing using a CRISPR-SpRY toolbox. Nature Plant. 2020,</br>
(2)Xuelian Zheng, Caiyan Qi, Lijia Yang, Quan Quan, Binglin Liu, Zhaohui Zhong, Xu Tang, Tingting Fan, Jianping Zhou and Yong Zhang. The improvement of CRISPR-Cas9 system with ubiquitin-associated domain fusion for efficient plant genome editing. Front. Plant Sci, 2020, 11:621. doi:10.3389/fpls.2020.00621.</br>
(3)Xu Tang, Simon Sretenovic, Qiurong Ren, Xinyu Jia, Mengke Li, Tingting Fan, Desuo Yin, Shuyue Xiang, Yachong Guo, Li Liu, Xuelian Zheng, Yiping Qi, Yong Zhang. Plant prime editors enable precise gene editing in rice cells. Molecular Plant. 2020, 13:667-670.</br>
(4)Meiling Ming, Qiurong Ren, Changtian Pan, Yao He, Yingxiao Zhang, Shishi Liu, Zhaohui Zhong, Jiaheng Wang, Aimee A. Malzahn1, Jun Wu, Xuelian Zheng, Yong Zhang, Yiping Qi. CRISPR–Cas12b enables efficient plant genome engineering. Nature Plants. 2020, 6:202–208</br>
(5)Zhong, Zhaohui#; Liu, Shishi#; Liu, Xiaopei; Liu, Binglin; Tang, Xu; Ren, Qiurong; Zhou, Jianping; Zheng, Xuelian; Qi, Yiping*; Zhang, Yong*; Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome Editing, Rice, 2020, 13: 8.</br>
(6)Tang, Xu#; Ren, Qiurong#; Yang, Lijia; Bao, Yu; Zhong, Zhaohui; He, Yao; Liu, Shishi; Qi, Caiyan; Liu, Binglin; Wang, Yan; Sretenovic, Simon; Zhang, Yingxiao; Zheng, Xuelian; Zhang, Tao*; Qi, Yiping*; Zhang, Yong*; Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing , Plant Biotechnology Journal, 2019, 17(7): 1431-1445.</br>
(7)Malzahn AA, Tang X, Lee K, Ren Q, Sretenovic S, Zhang Y, Chen H, Kang M, Bao Y, Zheng X, Deng K, Zhang T, Salcedo V, Wang K, Zhang Y, Qi Y. Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis. BMC Biology, 2019, 17(1):9.</br>
(8)Qiurong Ren, Zhaohui Zhong, Yan Wang, Qi You, Qian Li, Mingzhu Yuan, Yao He,Caiyan Qi, Xu Tang, Xuelian Zheng, Tao Zhang, Yiping Qi, Yong Zhang. Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing. Front. Plant Sci, 2019, 10:1173.</br>
(9)WANG Bo, ZHONG Zhaohui, ZHANG Huanhuan, WANG Xia, LIU Binglin, YANG Lijia, HAN Xiangyan, YU Deshui, ZHENG Xuelian, WANG Chunguo, SONG Wenqin, CHEN Chengbin, ZHANG Yong. Targeted Mutagenesis of NAC Transcription Factor Gene, OsNAC041, Leading to Salt Sensitivity in Rice. Rice Science, 2019, 26(2): 98-108.</br>
(10)Zhou J, Xin X, He Y, Chen H, Li Q, Tang X, Zhong Z, Deng K, Zheng X, Akher SA, Cai G, Qi Y, Zhang Y. Multiplex QTL editing of grain-related genes improves yield in elite rice varieties. Plant Cell Reports, 2019, 38: 475–485.</br>
(11)Jianping Zhou, Zhaohui Zhong, Hongqiao Chen, Qian Li, Xuelian Zheng, Yiping Qi, and Yong Zhang. Knocking Out MicroRNA Genes in Rice with CRISPR-Cas9. 2019, Plant Genome Editing with CRISPR Systems: Methods and Protocols, Methods in Molecular Biology, vol. 1917</br>
(12)Zheng X, Yang L, Li Q, Ji L, Tang A, Zang L, Deng K, Zhou J, Zhang Y, MIGS as a Simple and Efficient Method for Gene Silencing in Rice, Front. Plant Sci, 2018, 9:662.</br>
(13)Tang, Xu; Liu, Guanqing; Zhou, Jianping; Ren, Qiurong; You, Qi; Tian, Li; Xin, Xuhui; Zhong, Zhaohui; Liu, Binglin; Zheng, Xuelian; Zhang, Dengwei; Malzahn, Aimee; Gong, Zhiyun; Qi, Yiping; Zhang, Tao; Zhang, Yong. A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice. Genome Biology. 2018, 19:84.</br>
(14)Zhong, Zhaohui; Zhang, Yingxiao; You, Qi; Tang, Xu; Ren, Qiurong; Liu, Shishi; Yang, Lijia; Wang, Yan; Liu, Xiaopei; Liu, Binglin; Zhang, Tao; Zheng, Xuelian; Le, Ysa; Zhang, Yong; Qi, Yiping, Plant genome editing using FnCpf1 and LbCpf1 nucleases at redefined and altered PAM sites, Molecular Plant, 2018, 7:999-1002.</br>
(15)周建平,吉琳艺,何瑶,郑雪莲,罗樊,蔡光泽,张勇.利用CRISPR-Cas9系统高效创制水稻品系“17318”的OsGW2、OsGN1a基因定向突变体.西昌学院学报(自然科学版), 2018, 32:1-5.</br>
(16)Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng XL, Voytas DF, ZhongZH, Chen YY, Ren QR, Li Q, Kirkland ER, Zhang Y, Qi Y, A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants, Nature Plants, 2017, 3(7): 17018</br>
(17)Zhou JP#, Deng KJ#, Cheng Y, Zhong ZH, Tian L, Tang X, Tang AT, Zheng XL, Zhang T, Qi YP, Zhang Y*, CRISPR-Cas9 Based Genome Editing Reveals New Insights into MicroRNA Function and Regulation in Rice, Frontiers in Plant Science, 2017, 8:1598 </br>
(18)Liu Y, Sun G, Zhong ZH, Ji LY, Zhang Y, Zhou JP, Zheng XL*, Deng KJ*. Overexpression of AtEDT1 promotes root elongation and affects medicinal secondary metabolite biosynthesis in roots of transgenic Salvia miltiorrhiza. Protoplasma, 2017, 254(4):1617-1625 </br>
(19)Wei T, Deng K, Zhang Q, Gao Y,Liu Y, Yang M, Zhang L, Zheng X, Wang C, Liu Z, Chen C* and Zhang Y*. Modulating AtDREB1C Expression Improves Drought Tolerance in Salvia miltiorrhiza. Front Plant Sci. 2017, 8: 52.</br>
(20)刘玉; 孙耿; 仲昭晖; 郑雪莲(*); 邓科君(*), 烟草中过表达丹参SAMDC基因增强其抗旱性（英文）, 农业生物技术学报, 2017.3, (05)：729~738.</br>
(21)Tang Xu*, Zheng Xuelian*, Qi Yiping, Zhang Dengwei, Cheng Yan, Tang Aiting, Daniel F. Voytas, Zhang Yong, A single transcript CRISPR-Cas9 system for efficient genome editing in plants, Molecular Plant, 2016, 9(7): 1088~1091</br>
(22)Zheng Xuelian, Yang Shixin, Zhang Dengwei, Zhong Zhaohui, Tang Xu, Deng Kejun, Zhou Jianping, Qi Yiping, Zhang Yong, Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism, Plant Cell Reports, 2016, 35(7): 1545~1554</br>
(23)Zheng Xuelian, Zhou Jianping, Zang Lili, Tang Aiting, Liu Dongqing, Deng Kejun, Zhang Yong, Genetic and epigenetic alterations induced by different levels of rye genome integration in wheat recipient. Genetics and Molecular Research, 2016, 15(2): </br>
(24)Deng K，Zang L，Lan X，Zhong Z，Xiong B，Zhang Y，Zheng X(*). Antioxidant components from Cape Gooseberry. Journal of Food Processing and Preservation. 2016 40(5): 893-898.</br>
(25)Deng K，Lan X，Sun G, Ji L, Zheng X(*). Determination of sulfonamide residues in chicken liver using high-performance liquid chromatography. Food Analytical Methods. 2016 9,(12): 3337–3344</br>
(26)Zheng Xuelian, Li Qian, Liu Dongqing, Zang Lili, Zhang Kaiyue, Deng Kejun, Yang Shixin, Xie Zhengyang, Tang Xu, Qi Yiping, Zhang Yong, Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum. Plant Cell Reports, 2015, 34(11): 1873~1884</br>
(27)Levi G. Lowder, Zhang Dengwei, Nicholas J. Baltes, Joseph W. Paul III, Tang Xu, Zheng Xuelian, Daniel F. Voytas, Tzung-Fu Hsieh, Zhang Yong, Qi Yiping, A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation, Plant Physiology, 2015, 169: 971~985</br>
(28)Liu Yu, Yang Shixin, Cheng Yan, Liu Dongqing, Zhang Yong, Deng Kejun, Zheng Xuelian*, Production of Herbicide-Resistant Medicinal Plant Salvia miltiorrhiza Transformed with the Bar Gene. Appl Biochem Biotechnol, 2015, 177: 1456~1465</br>
(29)Shan Qiwei, Wang Yanpeng, Chen Kunling, Liang Zhen, Li Jun, Zhang Yi, Zhang Kang, Liu Jinxing, Voytas Daniel F, Zheng Xuelian, Zhang Yong, Gao Caixia, Molecular Plant, 2013, 6(4): 1365~1368</br>
(30)Zhang Mi*, Zheng Xuelian*, Song Shuiqing, Zeng Qiwei, Hou Lei, Li Demou, Zhao Juan, Wei Yuan, Li Xianbi, Luo Ming, Xiao Yuehua, Luo Xiaoying, Zhang Jinfa, Xiang Chengbin, Pei Yan, Nature Biotechnology, 2011, 29(5): 453~45</br>
授权发明专利：</br>
(1)张勇，郑雪莲，邓科君，唐旭，章登位，一种CRISPR/Cas9单一转录单元定向修饰骨架载体及其应用，2015-7，中国，ZL1.1</br>
(2)邓科君、郑雪莲、张勇，一种基于液体培养基的白芨种苗生产方法，2015-12，中国，ZL7.2</br>
(3)郑雪莲，张勇，邓科君，臧黎黎，一种基于人工tasiRNA途径的水稻RNAi骨架载体构建及应用，2016-2，中国，ZL5.5</br>
(4)郑雪莲，张勇，邓科君，仲昭辉，一种用于TALEN高效构建的双RVD单元模块库及TALEN构建方法，2016-5，中国，ZL8.0</br>
(5)周建平，张勇，郑雪莲，唐旭，程燕，邓科君，一种水稻miRNA定向敲除的方法，2016-9，中国，ZL3.6</br>
(6)汤丽霞、李思扬、张勇、郑雪莲、刘炳麟、邹佳佳，高催化活性的PET水解酶突变体，2017-10，中国，ZL8.4
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