单志,男,1979年出生,学硕导师
工作单位 生命科学学院
单位电话
电子邮箱 mesopig@hotmail.com
招生专业 【学硕】:071010生物化学与分子生物学，【专硕】:086000生物与医药
◆个人简历
本科就读于山东农业大学，硕士和博士攻读于四川农业大学，加拿大卡普顿大学Verschuren Centre博士后。主要从事自由基生物学（活性氧、活性氯对生物大分子和细胞的影响）和纳米生物技术（磁性和贵金属等纳米材料合成及在分离纯化和分析检测方面的应用）方面研究。
◆工作经历
2006-今 四川农业大学生命科学学院
◆教育经历
山东农业大学---生物技术专业 学士
四川农业大学---生物物理专业 硕士
四川农业大学---生化与分子生物学专业 博士
加拿大Cape Breton University大学Verschuren Centre博后
◆研究领域
主要研究方向：纳米生物技术
主要研究兴趣：（1）光学/磁学纳米材料的合成及其在生物分离/生物分析领域的应用研究（如生物大分子分离纯化、生物活性分子检测、环境激素检测及脱除）；（2）自由基和DNA损伤研究。
◆发表论文
1.Zhi Shan, Mingsheng Lu, Dennis E Curry, Stefanie Beale, Stephen Campbell, Kristin M Poduska, Craig Bennett, Ken D Oakes, Xu Zhang, Regenerative nanobots based on magnetic layered double hydroxide for azo dye removal and degradation, Chem. Commun., 2017,53, 10456-10458
2.Li Wang, Yanni Miao, Mingsheng Lu, Zhi Shan, Shan Lu, Jiaojiao Hou, Qiumei Yang, Xinle Liang, Tao Zhou, Dennis Curry, Ken Oakes, Xu Zhang, Chloride-accelerated Cu-Fenton chemistry for biofilm removal, Chem. Commun., 2017,53, 5862-5865
3.Mingsheng Lu, Zhi Shan, Kori Andrea, Bruce MacDonald, Stefanie Beale, Dennis E Curry, Li Wang, Shujun Wang, Ken D Oakes, Craig Bennett, Wenhui Wu, Xu Zhang, Chemisorption Mechanism of DNA on Mg/Fe Layered Double Hydroxide Nanoparticles: Insights into Engineering Effective SiRNA Delivery Systems, Langmuir, 2016, 32 (11), pp 2659–2667
4.Zhi Shan, Mingsheng Lu, Li Wang, Bruce MacDonald, Judy MacInnis, Martin Mkandawire, Xu Zhang, Ken D Oakes, Chloride accelerated Fenton chemistry for the ultrasensitive and selective colorimetric detection of copper, Chem. Commun., 2016,52, 2087-2090
5.Dennis Curry, Amanda Cameron, Bruce MacDonald, Collins Nganou, Hope Scheller, James Marsh, Stefanie Beale, Mingsheng Lu, Zhi Shan, Rajendran Kaliaperumal, Heping Xu, Mark Servos, Craig Bennett, Stephanie MacQuarrie, Ken D Oakes, Martin Mkandawire, Xu Zhang, Adsorption of doxorubicin on citrate-capped gold nanoparticles: insights into engineering potent chemotherapeutic delivery systems, Nanoscale, 2015,7, 19611-19619
6.Zhi Shan, Youjun Jiang, Mengyu Guo, J Craig Bennett, Xianghai Li, Hefeng Tian, Ken Oakes, Xu Zhang, Yi Zhou, Qianming Huang, Huaping Chen, Promoting DNA loading on magnetic nanoparticles using a DNA condensation strategy, Colloids and Surfaces B: Biointerfaces, 2015, 125, 247-254
7.Huang QM. Zhan MM, Cheng ZZ, Su GC1, Wang AY1, Chen HP1, Shan Z, Yang ZS, Genetic relationships analysis of olive cultivars grown in China, Genet. Mol. Res. 2015, 14 (2): 5958-5969
8.Yan Liao, Chun-mei Li, Hui Chen, Qi Wu, Zhi Shan, Xue-yi Han, Site-directed mutagenesis improves the thermostability and catalytic efficiency of Aspergillus niger N25 phytase mutated by I44E and T252R, Applied Biochemistry and Biotechnology, 2013, 171, 4, 900–915
9.Xianxiang Wang, Yanying Wang, Hanbing Rao, Zhi Shan, A Sensitive fluorescent assay for trypsin activity in biological samples using BSA-Au nanoclusters, J. Braz. Chem. Soc. [online]. 2012, vol.23, n.11, 2011-2015.
10.Ken Oakes, Zhi Shan, Rajendran Kaliaperumal, Shine Xu Zhang, Martin Mkandawire, Nanotechnology in Contemporary Mine Water Issues. In: Hu A., Apblett A. (eds) Nanotechnology for Water Treatment and Purification. Lecture Notes in Nanoscale Science and Technology, 2014, vol 22, 307-361, Springer, Cham
11.Zhi Shan, Wan-Shen Yang, Xu Zhang, Qian-Ming Huang, Hui Ye. Preparation and Characterization of Carboxyl-Group Functionalized Superparamagnetic Nanoparticles and the Potential for Bio-Applications. Journal of the Brazilian Chemical Society, 2007, 18, 1329-1335 1.343
12.Zhi Shan, Wanshen Yang, Xu Zhang, Bio-Separation Based on Functionalized Magnetic Nanoparticles. Nanotechnology Research Journal, 2009, 2, 1/2, 39-72
13.Zhi Shan, Wanshen Yang, Xu Zhang, Preparation of Magnetic Nanoparticles and Application to Isolation of Cells and Extraction of Biomacromolecules from Biological Samples. Book chapter, chapter 2 in Progress in Nanoparticles Research, Editors: Chris Thomas Frisiras, Nova Publishers, 2008, 57-90.
14.Zhi Shan, Qi Wu, Xianxiang Wang, Zhongwu Zhou, Ken D. Oakes, Xu Zhang, Qianming Huang and Wanshen Yang, Bacteria capture, lysate clearance, and plasmid DNA extraction using pH-sensitive multifunctional magnetic nanoparticles. Analytical Biochemistry, 398, 1, 2010, 120-122. 3.236
15.Zhi Shan, Chenglei Li, Xu Zhang, Ken D. Oakes, Mark R. Servos, Qi Wu, Hui Chen, Xianxiang Wang, Qianming Huang, Yi Zhou and Wanshen Yang, Temperature-dependent selective purification of plasmid DNA using magnetic nanoparticles in an RNase-free process. Analytical Biochemistry, 2011, 412(1): 117-119. 3.236
16.Zhi Shan, Zhongwu Zhou, Hui Chen, Zhiming Zhang, Yi Zhou, Anxiang Wen, Ken D. Oakes, Mark R. Servos, PCR-ready human DNA extraction from urine samples using magnetic nanoparticles, Journal of Chromatography B, 2012, 881– 882: 63–68 2.971
17.Zhi Shan, Xianghai Li, Yaying Gao, Xianxiang Wang, Chenglei Li, Qi Wu, Application of Magnetic Hydroxyapatite Nanoparticles for Solid Phase Extraction of plasmid DNA, Analytical Biochemistry (2012), doi: 10.1016/j.ab.2012.03.013
18.杨群峰，刘建云，陈华萍，王显祥，黄乾明，单志*，贵金属纳米团簇的制备及在生物检测中的应用，化学进展, 2011, 23(5): 880-892.
19.单志，吴宏亮，李成磊等，改良SDS法提取多种植物基因组DNA. 广东农业科学, 2011,38(8), 113-115.
20.单志，周中午，吴琦等，磁珠分离法快速提取大肠杆菌质粒DNA. 四川农业大学学报,2011,29(2), 230-234.
21.单志; 杨婉身; 张旭; 黄乾明; 叶辉. 正交实验法优化羧基纳米磁性粒子的合成条件. 绵阳师范学院学报; 2006, 02: 56-59
22.XianXiang Wang, ZhongKe Yang, Zhi Shan, Xi Pen and QianMing Huang, Preparation of giant unilamellar CdTe quantum dot vesicles and their metabolic pathway in vivo. Science China Chemistry, 2010, 53(8): 1718-1722
23.王显祥; 黄娟; 靳茹文; 杨中科; 单志; 杨婉身. 水相合成CdX(X=Te,Te/CdS,Te/ZnS)红色量子点及毒性效应. 化学学报, 2009,67(17):2025-2030
24.王显祥; 钟娇懿; 刘怡; 温安祥; 单志; 杨婉身;磁性荧光双功能纳米材料的合成及其用于葡萄糖的分离检测化学学报, 2010, 68(20): 2063~2068
25.沈茂; 李双江; 吴琦; 单志; 陈惠;磁修饰酵母对直接蓝71的生物吸附研究, 中国皮革, 2010,39(17): 1-4
26.王显祥; 杨中科; 单志; 彭西; 黄乾明;大单室CdTe量子点脂质体的制备及其体内代谢途径，中国科学:化学, 2010, 40（10）：1503-1508
27.吴琦; 容杰; 单志; 陈惠; 杨婉身;水溶性有机溶剂对Fe3O4磁性纳米粒子过氧化物酶样活性的影响生物工程学报, 2009,12
28.吴琦; 单志; 沈茂; 李双江; 陈惠;磁修饰酵母对直接大红染料的生物吸附. 生物工程学报, 2009,10
29.王显祥,王森,杨婉身,单志, 超声合成Fe3O4@SiO2复合纳米磁性粒子用于质粒DNA的提纯.化学学报, 2009 (1): 54—58
30.XianXiang Wang, Shuo Huang, Zhi Shan, Wanshen Yang, Preparation of Fe3O4@Au nano-composites by self-assembly technique for immobilization of glucose oxidase Chinese Science Bulletin, 2009, 54(7): 1176~1181
31.王显祥 黄硕 单志 杨婉身, 自组装制备Fe3O4@Au复合纳米粒子用于固定化葡萄糖氧化酶. 科学通报, 2009 54 (4): 430-435
32.叶辉; 杨婉身; 金丽琴; 廖敏; 单志; 纳米磁性干扰素脂质体在裸鼠移植性人肝癌靶向治疗过程中对VEGF和Caspase-3表达的影响. 中国药理学通报, 2008, 06, 730-735
33.杨婉身; 张寒飞; 黄乾明; 单志; 量子点作为生物荧光标记物的研究进展. 绵阳师范学院学报, 2006, 02, 1-7
34.陈惠; 王红宁; 杨婉身; 赵海霞; 吴琦; 单志; 植酸酶phyA~m基因结构延伸突变改善酶的热稳定性. 生物工程学报, 2005, 06, 983-987
35.陈惠; 王红宁; 杨婉身; 赵海霞; 吴琦; 单志; F43Y及I354M,L358F定点突变对植酸酶热稳定性及酶活性的改善. 中国生物化学与分子生物学报, 2005, 04, 516-520
◆专著教材
《生物化学实验》，芶琳 单志主编，西南交通大学出版社，成都，2015年8月第2版
《基础生物化学》，陈惠主编，单志、金青、陈疏影副主编，全国高等农林院校十二五规划教材，2014.1，北京，中国农业出版社
《生物化学实验教程》，单志、吴琦主编，全国高等农林院校十三五规划教材，2017.7，北京，中国农业出版社
◆指导学生
2009届 周中午（共同）
2010届 胡容 （共同）
2011届 李祥海 姜友军（共同）
2012届 田鹤枫
2013届 强 岩
2014届 雷云龙
2016届 谢刘琴
2017届 祝茂琴
2018届 张转红