一、个人简介：
　　吴健，1986年生，博士，讲师（校聘副教授），硕士生导师。主讲本科生《细胞遗传学》、《植物细胞工程与技术实验》和研究生《植物研究技术》等课程。长期从事油菜抗菌核病分子机理以及基因组编辑研究。
联系方式：E-mail：wu_jian@yzu.edu.cn; QQ：
　　招生方向：植物学
　　二、学习工作经历：
　　2005.09-2009.06，华中农业大学，植物科学技术学院，获农学学士学位
　　2009.09-2015.06，华中农业大学，植物科学技术学院，获农学博士学位
2015.07-2019.03，扬州大学，生物科学与技术学院，讲师（师资博士后）　
2019.03-今，扬州大学，生物科学与技术学院，讲师（校聘副教授）
　　三、主要研究方向：
油菜抗菌核病的遗传基础和分子机理
油菜基因组编辑
油菜基因组学
　　四、主讲课程：
　　《细胞遗传学》、《植物研究技术》、《植物细胞工程与技术实验》
　　五、发表相关论文：
First author and Corresponding author Publications
(1) Wu J, Chen C, Xian GY, Liu DX, Lin L, Yin SL, Sun QF, Fang YJ, Zhang H, Wang YP^(*). Engineering herbicide-resistant oilseed rape by CRISPR/Cas9-mediated cytosine base-editing. Plant Biotechnology Journal, 2020, https://doi.org/10.1111/pbi.13368 (IF₂₀₁₈ = 8.154，引用6次)
(2) Zhao Q^(#), Wu J^(#), Cai GQ, Yang QY, Shahid M, Fan CC, Zhang CY, Zhou YM^(*). A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus. Plant Biotechnology Journal, 2019, 17 (12):2313-2324 (IF₂₀₁₈ = 8.154, 引用6次)
(3) Wu J, Chen PP, Zhao Q, Cai GQ, Hu Y, Xiang Y, Yang QY, Wang YP, Zhou YM^(*). Co-location of QTL for Sclerotinia stem rot resistance and flowering time in Brassica napus. The Crop Journal, 2019, 7:227-237 (IF₂₀₁₈ = 3.179, 引用3次，封面文章)
(4) Wu J, Lin L, Xu ML, Chen PP, Liu DX, Sun QF, Ran LP, Wang YP^(*). Homoeolog expression bias and expression level dominance in resynthesized allopolyploid Brassica napus. BMC Genomics, 2018, 19(1):586 (IF₂₀₁₈ = 3.501, 引用21次)
(5) Sun QF, Lin L, Liu DX, Wu DW, Fang YJ, Wu J^(*), Wang YP^(*). CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L. International Journal of Molecular Sciences, 2018, 19 (9):2716 (IF₂₀₁₈ = 4.183, 引用24次)
(6) Wu J, Zhao Q, Yang QY, Liu H, Li QY, Yi XQ, Cheng Y, Guo L, Fan CC, Zhou YM^(*). Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus. Scientific Reports, 2016, 6:19007 (IF₂₀₁₅ = 5.228, 引用72次)
(7) Wu J^(#), Zhao Q^(#), Liu S^(#), Shahid M, Lan L, Cai GQ, Zhang CY, Fan CC, Wang YP, Zhou YM^(*). Genome-wide association study identifies new loci for resistance to Sclerotinia stem rot in Brassica napus. Frontiers in Plant Science, 2016, 7:1418 (IF₂₀₁₆ = 4.291, 引用29次)
(8) Wu J, Cai GQ, Tu JY, Li LX, Liu S, Luo XP, Zhou LP, Fan CC, Zhou YM^(*). Identification of QTLs for resistance to Sclerotinia stem rot and BnaC. IGMT5. a as a candidate gene of the major resistant QTL SRC6 in Brassica napus. Plos One, 2013, 8 (7):e67740 (IF₂₀₁₃ = 3.534, 引用86次)
(9) 吴健, 周永明, 王幼平^(*). 油菜与核盘菌互作分子机理研究进展. 中国油料作物学报, 2018, 40(5):721-729.
(10) 吴健, 涂江颖, 周永明^(*). 甘蓝型油菜抗菌核病研究进展. 中国油料作物学报, 2013, 35(5):608-615.
(11) 林俐，吴健^(*). 植物 MAPK 级联在逆境胁迫响应中的作用研究进展, 分子植物育种, 2018, 16(1): 280-288.
Co-author Publications（部分）
(1) Cai GQ, Fan CC, Liu S, Yang QY, LiuDX, Wu J, Li JW, Zhou YM, Guo L^(*), Wang XM^(*). Nonspecific phospholipase C6 increases seed oil production in oilseed Brassicaceae plants. New Phytologist, 2020, https://doi.org/10.1111/nph.16473.
(2) Du K, Xiao YY, Liu QE, Wu XY, Jiang JJ, Wu J, Fang YJ, Xiang Y, Wang YP^(*). Abnormal tapetum development and energy metabolism associated with sterility in SaNa-1A CMS of Brassica napus L. Plant Cell Reports, 2019, 38 (5):545-558.
(3) Sun QF, Xue JY, Lin L, Liu DX, Wu J, Jiang JJ, Wang YP^(*). Overexpression of soybean transcription factors GmDof4 and GmDof11 significantly increase the oleic acidcontent in seed of Brassica napus L. Agronomy, 2019, 8 (10):222
(4) Ling J, Li RJ, Nwafor CC, Cheng JL, Li MT, Xu Q, Wu J, Gan L, Yang QY, Liu C, Chen M, Zhou YM, Cahoon EB, Zhang CY^(*). Development of iFOX-hunting as a functional genomic tool and demonstration of its use to identify early senescence-related genes in the polyploid Brassica napus. Plant Biotechnology Journal, 2018, 16 (2):591-602. https://doi.org/10.1111/pbi.12799
(5) Du K, Liu QE, Wu XY, Jiang JJ, Wu J, Fang YJ, Li AM, Wang YP^(*). Morphological structure and transcriptome comparison of the cytoplasmic male sterility line in Brassica napus (SaNA-1A) derived from somatic hybridization and its maintainer line SaNa-1B. Frontiers in Plant Science, 2016, 7:1313. https://doi.org/10.3389/fpls.2016.01313
(6) Liu S, Fan CC, Li JN, Yang QY, Wu J, Yi XQ, Zhang CY, Zhou YM^(*). A genome-wide association study reveals novel elite allelic variations in the seed oilcontent of Brassica napus. Theoretical and Applied Genetics, 2016, 129(6): 1203-1215. https://doi.org/10.1007/s00122-016-2697-z
(7) Zhang J, Mason AS, Wu J, Liu S, Zhang XC, Luo T, Redden R, Batley J, Hu LY^(*), Yan GJ^(*). Identification of putative candidate genes for water stress tolerance in canola (Brassica napus). Frontiers in Plant Science, 2015, 6:1058. https://doi.org/10.3389/fpls.2015.01058
(8) Li QY, Yin M, Li YP, Fan CC, Yang QY, Wu J, Zhang CC, Wang H, Zhou YM^(*). Expression of Brassica napusTTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes. Journal of Experimental Botany, 2015, 66(19):5821-5836. https://doi.org/10.1093/jxb/erv287
(9) Yin TZ, Pan G, Liu H, Wu J, Li YP, Zhao ZX, Fu TD, Zhou YM^(*)．The chloroplast ribosomal protein L21 gene is essential for plastid development and embryogenesis in Arabidopsis. Planta, 2012, 235:907-921. https://doi.org/10.1007/s00425-011-1547-0
(10) Cai G, Yang QY, Yang Q, Zhao ZX, Chen H, Wu J, Fan CC, Zhou YM^(*)．Identification of candidate genes of QTLs for seed weight in Brassica napus through comparative mapping among Arabidopsis and Brassica species. 2012, BMC genetics, 13:105. https://doi.org/10.1186/1471-2156-13-105
六、专利
(1) 吴健, 王幼平, 林俐, 孙勤富, 刘东晓, 方玉洁, 蒋金金, 吴德伟, 一种受核盘菌诱导的油菜启动子pBnGH、鉴定方法和应用, 2.2.
(2) 吴健, 霍诗诗, 王幼平, 张辉, 倪萍, 基于基因编辑的甘蓝型油菜BnALS1突变型基因、蛋白及其应用, 3.0.
(3) 孙勤富, 王幼平, 吴健, 蒋金金, 李玉兰, 一种快速高效的甘蓝型油菜毛状根诱导及培养方法, ZL3.8.
(4) 孙勤富, 王幼平, 吴健, 蒋金金, 林俐, 方玉洁, 一种基于真空渗入的甘蓝和甘蓝型油菜叶片瞬时表达的方法, 0.0.
(5) 方玉洁, 耿玉璐, 王幼平, 张盼, 张帅, 赵璇, 吴健, 蒋金金, BnLAX1.c基因、蛋白及其在控制甘蓝型油菜株型中的应用，20**。
(6) 蒋金金，郭图丽，王幼平，王月，王霞琴，蒙贵生，方玉洁，吴健，吴德伟，Bna-miR156d在控制甘蓝型油菜多分枝中的应用，1.9
七、科研项目
(1). 利用跨界RNA干扰技术提高油菜菌核病抗性的研究，国家自然科学基金面上项目，2021.01-2024.12，59万，主持人
(2). 利用基因编辑技术创制抗除草剂油菜新种质，省农业科技自主创新，2020.08-2022.07，30万，主持人
(3). 甘蓝型油菜MKK9基因在菌核病抗性中的功能分析，国家自然科学基金青年基金，2017.01-2019.12，22万，主持人
(4). 国家科技重大专项“转基因油菜新品种培育及产业化研究”子课题—黄籽油菜转基因油菜新品种培育，2018.01-2020.12，58.75万，子课题负责人
(5). 甘蓝型油菜MAPK信号通路在抗菌核病反应中的功能分析，中国博士后科学基金特别资助，2016.01-2018.07，15万，主持人
(6). MKK9在甘蓝型油菜抗菌核病反应中的功能研究，中国博士后科学基金面上资助，2015.07-2017.07，5万，主持人
(7). 基于基因组编辑技术创制抗菌核病甘蓝型油菜新种质，江苏省现代农业重点项目，2018.07- 2021.6，100万，项目骨干