高翔
东北师范大学生命科学学院
-->
职　　称：副教授
研究方向：经济作物重要农艺形状转录调控网络解析
办公电话：
办公地点：综合实验楼305
电子邮件：gaox891@nenu.edu.cn



个人简历
高翔，副教授，博士生导师，东师仿吾青苗学者，主要从事经济植物重要农艺性状转录调控研究。2010年毕业于东北师范大学生命科学学院，获细胞生物学博士学位；2016年12月至2017年12月在加拿大英属哥伦毕业大学植物系开展访学研究。目前，主持国家重点研发计划子课题1项、国家自然科学基金青年项目1项、吉林省科技厅项目3项，参与科技部国际合作项目和国家自然科学基金面上项目多项；参编教材2部；参与培育多个花卉新品种，获得发明专利2项；以第2完成人获吉林省科技进步一等奖和二等奖各1项。近5年，在J Exp Bot等杂志发表SCI论文26篇，其中通讯作者19篇。Front. Plant Sci杂志Reviewer Editor，Journal of Experimental Botany等杂志审稿人，国家自然科学基金网评审稿人。近期开展工作：经济植物重要农艺性状调控网络解析及其进化意义探究方向（1）依托课题组长期的研究优势，进一步解析观赏花卉香雪兰花朵多种重要农艺性状的转录调控网络，建立香雪兰瞬时及稳定遗传转化体系。首先，筛选并验证控制除花色苷以外的其他类黄酮物质（如黄酮醇和原花青素）生物合成的转录因子基因，并解析相关的调控网络。其次，筛选与控制香雪兰花香形成相关的挥发性萜类合酶基因表达的转录因子基因的功能。最后，厘清在香雪兰花朵发育过程中控制上述各种次生代谢物质合成的转录调控网络之间的复杂的交互作用关系。（2）基于香雪兰花色苷转录调控模型研究基础，对比其他被子植物，包括基础双子叶植物（耧斗菜和/荷花），单子叶植物（香雪兰和/蝴蝶兰），核心双子叶植物（大豆和/烟草）。揭示MBW复合物中MYB转录因子在被子植物进化过程中的进化机制。（3）依托承担的国家科技计划项目国家重点研发计划项目，重点筛选控制大豆和番茄异黄酮、花色苷和原花青素等次生代谢物质合成的MYB和MADS-box等转录因子基因，解析它们参与的转录调控网络。（4）与加拿大英属哥伦比亚大学（UBC）植物系的Quentin Cronk教授合作，探究花色和花香等花朵性状影响植物传粉者选择行为的分子机制，揭示花色和花香性状的生态和进化意义。主要科研项目1.长白山特色植物耧斗菜花香形成分子机制及其与传粉者的协同进化，吉林省科技厅自然科学基金（主题引导项目），2019.1-2021.12,70万。2.调控重要农艺性状的转录因子的鉴定及功能分析Ⅱ，国家重点研发计划子课题，2016.7-2020.12,80万。3.调控香雪兰挥发性萜类合成的转录因子克隆及其对萜类合酶基因表达和花香释放的影响，2014.1-2016.12，24万。欢迎对植物分子生物学及遗传与进化方向感兴趣的同学加入我们课题组！！！！！发表论文：(1)Li Y, Shan X, Zhou L, Gao R,Yang S, Wang S, Wang L and Gao X（高翔）* (2019) The R2R3-MYB Factor FhMYB5 From Freesia hybrida Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis. Front. Plant Sci. 9:1935.(2)Jing Chen, Baofeng Liu, Zhongzhou Yang, Jiao Qu, Hongwei Xun, Runzhi Dou, Xiang Gao（高翔）*, and Li Wang*, Phenotypic, transcriptional, physiological and metabolic responses to carbon nanodots exposure in Arabidopsis thaliana (L.),2018, Environ. Sci.: Nano, 2018, 5, 2672(3)Chen J, Dou R, Yang Z, You T, Gao X（高翔）*, Wang L*, Phytotoxicity and bioaccumulation of zinc oxide nanoparticles in rice (Oryza sativa L.). Plant Physiol Biochem. 2018 Sep; 130:604-612.(4)Shi H, Dong S, Zhang X, Chen X, Gao X（高翔）*, Wang L*. Phage vaccines displaying YGKDVKDLFDYAQE epitope induce protection against systemic candidiasis in mouse model. Vaccine. 2018 Sep 11;36(38):5717-5724.(5)Gao F, Liu B, Li M, Gao X, Fang Q, Liu C, Ding H, Wang L*, Gao X（高翔）*, Identification and Characterization of Terpene Synthase Genes Accounting for the Volatile Terpene Emissions in Flowers of Freesia hybrida, J Exp Bot. 2018 Volume 69, Issue 18, Pages 4249–4265(6)Zhigang Ju, Wei Sun, Xiangyu Meng, Lingjie Liang, Yueqing Li, Tongtong Zhou, Huan Shen, Xiang Gao（高翔）*, Li Wang*, Isolation and functional characterization of two 5-Oglucosyltransferases related to anthocyanin biosynthesis from Freesia hybrida, Plant Cell, Tissue and Organ Culture, (2018) 135: 99. (7)Yicun Wang, Hongxi Shi, Shuai Dong, Yan Li, Meng Wang, Yanyan Huai, Xintong Zhang, Xi Chen, Chuanbin Mao, Xiang Gao（高翔）* and Li Wang*, Nontoxic engineered virus nanofiber as an efficient agent for prevention and detection of fungal infection, Nano Research, 11: 2248, 2018(8)Dong S, Shi H, Zhang X, Chen X, Cao D, Mao C, Gao X*（高翔）, Wang L*, Difunctional bacteriophage conjugated with photosensitizers for Candida albicans-targeting photodynamic inactivation, International Journal of Nanomedicine, 13: 2199—2216, 2018(9)Tingting You, Dandan Liu, Jing Chen, Zhongzhou Yang, Runzhi Dou, Xiang Gao（高翔）*, Li Wang* (2018) Effects of metal oxide nanoparticles on soil enzyme activities and bacterial communities in two different soil types. J SOIL SEDIMENT, 18: 211.(10)Wei Sun, Xiangyu Meng, Lingjie Liang, Yueqing Li, Tongtong Zhou, Xinquan Cai, Li Wang, Xiang Gao（高翔）*(2017) Overexpression of a Freesia hybrida flavonoid 3-O-glycosyltransferase gene, Fh3GT1, enhances transcription of key anthocyanin genes and accumulation of anthocyanin and flavonol in transgenic petunia (Petunia hybrida). In Vitro Cell. Dev.Biol.—Plant, ?53: 478.(11)Li Y, Liu X, Cai X, Shan X, Gao R, Yang S, Han T, Wang S, Wang L* and Gao X（高翔）* (2017) Dihydroflavonol 4-Reductase Genes from Freesia hybrida Play Important and Partially Overlapping Roles in the Biosynthesis of Flavonoids. Front. Plant Sci. 8:428.(12)Li S, Xia Q, Wang F*, Yu X, Ma J, Kou H, Lin X, Gao X（高翔）* and Liu B (2017) Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice. Front. Plant Sci. 8:363(13)Shuai Dong, Hongxi Shi, Donghui Cao, Yicun Wang, Xintong Zhang, Yan Li, Xiang Gao（高翔）*, Li Wang*, Novel nanoscale bacteriophage-based single-domain antibodies for the therapy of systemic infection caused by Candida albicans, Scientific Reports, (2016) | 6:32256(14)Yueqing Li, Xiaotong Shan, Ruifang Gao, Song Yang, Shucai Wang, Xiang Gao（高翔）*, Li Wang*, Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis, Scientific Reports, (2016) 6:30514(15)Jing Chen, Runzhi Dou, Zhongzhou Yang, Xiaoping Wang, Chuanbin Mao, Xiang Gao（高翔）*, Li Wang*, The Effect and Fate of Water-Soluble Carbon Nanodots in Maize (Zea mays L.), Nanotoxicology, 2016, 10(6):818-828.(16)Wei Sun, Lingjie Liang, Xiangyu Meng, Yueqing Li, Fengzhan Gao, Xingxue Liu, Shucai Wang, Xiang Gao（高翔）* and Li Wang*，Biochemical and Molecular Characterization of a Flavonoid 3-O-glycosyltransferase Responsible for Anthocyanins and Flavonols Biosynthesis in Freesia hybrida，Front. Plant Sci. (2016) 7:410.(17)Wei Sun, Xiangyu Meng, Lingjie Liang, Wangshu Jiang, Yafei Huang, Jing He, Haiyan Hu, Jonas Almqvist, Xiang Gao（高翔）*, Li Wang*, Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in Flavonoid Biosynthetic Pathway, (2015),PLoS ONE 10(3): e**.(18)Wang Y, Su Q, Dong S, Shi H, Gao X（高翔）*, Wang L*, Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice, Hum Vaccin Immunother. (2014) 10(4):1057-63.(19)Gao X（高翔）, Cao D, Liu J, Wang X, Geng S, Liu B, Shi D*, Tissue-Specific and Cation/Anion-Specific DNA Methylation Variations Occurred in C. virgata in Response to Salinity Stress, PLoS ONE,(2013) 8(11):e78426(20)Wang QM, Wang YZ, Sun LL, Gao FZ, Sun W, He J, Gao X（高翔）*, Wang L*, Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets, Plant Cell Rep, (2012) 31:1283–1296(21)Xiang Gao（高翔）, Dan Yang, Donghui Cao, Man Ao, Xin Sui, Qinmei Wang, J. N. Kimatu, Li Wang*, In Vitro Micropropagation of Freesia hybrida and the Assessment of Genetic and Epigenetic Stability in Regenerated Plantlets，J Plant Growth Regul,(2010) 29:257–267(22)Donghui Cao*, Xiang Gao（高翔）*, Jie Liu, Xiaoping Wang, Shujuan Geng, Chunwu Yang, Bao Liu, Decheng Shi*, Root-Specific DNA Methylation in Chloris virgata, a Natural Alkaline-Resistant Halophyte, in Response to Salt and Alkaline Stresses, Plant Mol Biol Rep, (2012) 30(5): 1102-1109 (*contributed equally to this work)(23)DH Cao*, X Gao（高翔）*, J Liu, JN Kimatu, SJ Geng, XP Wang, J Zhao, DC Shi, Methylation sensitive amplified polymorphism (MSAP) reveals that alkali stress triggers more DNA hypomethylation levels in cotton (Gossypium hirsutum L.) roots than salt stress, Afa J biotechnol, (2011) 10(82):18971-18980 (*contributed equally to this work)(24)Xiang GAO（高翔）, Yue-jun HUI, Yi-qun XUE, Yang FU, Man AO, Li WANG*, Specific Proteins in the Indirect Somatic Embryogenesis of Freesia Refracta,Chem Res Chinese U, (2007) 23(2):187-190(25)Xun H, Ma X, Chen J, Yang Z, Liu B, Gao X（高翔）, Li G, Yu J, Wang L, Pang J.Zinc oxide nanoparticle exposure triggers different gene expression patterns in maize shoots and roots. Environ Pollut. (2017) 229:479-488.(26)Xiaoping Wang, Xiyu Yang, Siyu Chen, Qianqian Li, Wei Wang, Chunjiang Hou, Xiang Gao（高翔）, Li Wang and Shucai Wang*, Zinc Oxide Nanoparticles Affect Biomass Accumulation and Photosynthesis in Arabidopsis. Front. Plant Sci. (2016) 6:1243.(27)Huai Y, Dong S, Zhu Y, Li X, Cao B, Gao X（高翔）, Yang M*, Wang L*, Mao C*. Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection, Adv Healthc Mater. (2016) doi: 10.1002/adhm.(28)Yicun Wang?, Zhigang Ju?, Binrui Cao, Xiang Gao（高翔）, Ye Zhu, Penghe Qiu, Hong Xu, Pengtao Pan, Huizheng Bao, Li Wang,* and Chuanbin Mao*, Ultrasensitive Rapid Detection of Human Serum Antibody Biomarkers by Biomarker-Capturing Viral Nanofibers, ACS NANO, (2015) 9(4): 4475-4483(29)Pengtao Pan?, Yicun Wang?, Ye Zhu?, Xiang Gao（高翔）, Zhigang Ju, Penghe Qiu, Li Wang*, Chuanbin Mao*, Nontoxic virus nanofibers improve the detection sensitivity for the anti-p53 antibody, a biomarker in cancer patients, Nano Research, (2015) 8(11): 3562–3570(30)Zhongzhou Yang, Jing Chen, Runzhi Dou, Xiang Gao（高翔）, Chuanbin Mao* and Li Wang*, Assessment of the Phytotoxicity of Metal Oxide Nanoparticles on Two Crop Plants, Maize (Zea mays L.) and Rice (Oryza sativa L.), Int. J. Environ. Res. Public Health (2015), 12: 15100–15109(31)Pan P, Han X, Li F, Fu Q, Gao X（高翔）, Sun H*, Wang L*, Detection of serum p53 antibodies from Chinese patients with papillary thyroid carcinoma using phage-SP-ELISA: correlation with clinical parameters, Endocrine. (2014) 47(2):543-9(32)Wang Y, Gao X（高翔）, Zhi Gang JU, Liu J, Dong S, Wang L*, Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis, Indian J Pathol Microbiol. (2013) 56(3):243-7(33)Qin-Mei Wang, Feng-Zhan Gao, Xiang Gao（高翔）, Fan-Yu Zou, Xin Sui, Meng Wang, Yue-Jun Hui, Li Wang*, Regeneration of Clivia miniata and assessment of clonal fidelity of plantlets, Plant Cell Tiss Org, (2012) 109(2):191-200(34)Sui X, Gao X（高翔）, Ao M, Wang Q, Yang D, Wang M, Fu Y, Wang L*, cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida, Plant Cell Rep, (2011) 30(7):1209-18(35)Wu J, Qiu T, Pan P, Yu D, Ju Z, Qu X, Gao X（高翔）, Mao C, Wang L*, Detection of serum anti-P53 antibodies from patients with colorectal cancer in China using a combination of P53- and phage-ELISA: correlation to clinical parameters, Asian Pac J Cancer Prev. (2011) 12(11):2921-4.(36)Yang Fu, Xiang Gao（高翔）,Yiqun Xue,Yuejun Hui,Fengqing Chen, Quanping Su and Li Wang. Voltile Compounds in the Flowers of Freesia Parental Species and Hybrids. Jounal of Integrative Plant Biology, 2007,49(12):1714-1718(37)Feng-Qing Chen, Yang Fu, De-Li Wang, Xiang Gao（高翔）, Li Wang*, The Effect of Plant Growth Regulators and Sucrose on the Micropropagation and Microtuberization of Dioscorea nipponica Makino, J Plant Growth Regul, (2007) 26(1):38-45

社会兼职




获奖情况 （数据来源：科学技术处、社会科学处）


2016-11-01 吉林省科学技术奖科技进步奖一等奖
2014-11-13 长春市科学技术进步奖一等奖
2011-12-31 吉林省科学技术进步奖一等奖
2008-11-06 吉林省科学技术进步二等奖


教学信息 （数据来源：教务处）


细胞工程


科研信息 （数据来源：科学技术处、社会科学处）


项目：
1. 调控重要农艺性状的转录因子的鉴定及功能分析Ⅱ，2016年
2. 基于p53抗体的肿瘤筛查生物检测剂开发，吉林省科技厅，2013年
3. 调控香雪兰挥发性萜类合成的转录因子克隆及其对萜类合酶基因表达和花香释放的影响，国家自然科学基金委员会，2013年
4. 香雪兰花青素合成相关基因克隆及其表达对花色形成的影响，吉林省科技厅，2012年
专著：
1. 细胞生物学实验指导，高等教育出版社，01-1年
论文：
1. Phenotypic, transcriptional, physiological and metabolic responses to carbon nanodot exposure in Arabidopsis thaliana (L.)，ENVIRONMENTAL SCIENCE-NANO，2018年
2. Phytotoxicity and bioaccumulation of zinc oxide nanoparticles in rice (Oryza sativa L.)，PLANT PHYSIOL BIOCH，2018年
3. Phage vaccines displaying YGKDVKDLFDYAQE epitope induce protection against systemic candidiasis in mouse model，VACCINE，2018年
4. Isolation and functional characterization of two 5-Oglucosyltransferases related to anthocyanin biosynthesis from Freesia hybrida，PLANT CELL TISS ORG，2018年
5. Identification and characterization of terpene synthase genes accounting for volatile terpene emissions in flowers of Freesia x hybrida，J EXP BOT，2018年
6. Difunctional bacteriophage conjugated with photosensitizers for Candida albicans-targeting photodynamic inactivation，INTERNATIONAL JOURNAL OF NANOMEDICINE，2018年
7. Nontoxic engineered virus nanofiber as an efficient agent for the prevention and detection of fungal infection，Nano Research ，2018年
8. Effects of metal oxide nanoparticles on soil enzyme activities and bacterial communities in two different soil types，J SOIL SEDIMENT，2018年
9. Overexpression of a Freesia hybrida flavonoid 3-O-glycosyltransferase gene, Fh3GT1, enhances transcription of key anthocyanin genes and accumulation of anthocyanin and flavonol in transgenic petunia (Petunia hybrida)，IN VITRO CELL DEV-PL，2017年
10. 玉米转录因子Pl表达载体的构建及大花君子兰遗传转化体系的建立，分子植物育种，2017年
11. Zinc oxide nanoparticle exposure triggers different gene expression patterns in maize shoots and roots，ENVIRON POLLUT，2017年
12. Dihydroflavonol 4-Reductase Genes from Freesia hybrida Play Important and Partially Overlapping Roles in the Biosynthesis of Flavonoids，FRONTIERS IN PLANT SCIENCE，2017年
13. Laser Irradiation-Induced DNA Methylation Changes Are Heritable and Accompanied with Transpositional Activation of mPing in Rice，FRONTIERS IN PLANT SCIENCE，2017年
14. Novel nanoscale bacteriophage-based single-domain antibodies for the therapy of systemic infection caused by candida albicans，SCIENTIFIC REPORTS，2016年
15. Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis，SCIENTIFIC REPORTS，2016年
16. Genetically Engineered Virus Nanofibers as an Efficient Vaccine for Preventing Fungal Infection，ADVANCED HEALTHCARE MATERIALS，2016年
17. Biochemical and Molecular Characterization of a Flavonoid 3-O-glycosyltransferase Responsible for Anthocyanins and Flavonols Biosynthesis in Freesia hybrida，FRONTIERS IN PLANT SCIENCE，2016年
18. The effect and fate of water-soluble carbon nanodots in maize (Zea mays L.)，NANOTOXICOLOGY，2016年
19. Zinc oxide nanoparticles affect biomass accumulation and photosynthesis in Arabidopsis，FRONTIERS IN PLANT SCIENCE，2016年
20. Assessment of the Phytotoxicity of Metal Oxide Nanoparticles on Two Crop Plants, Maize (Zea mays L.) and Rice (Oryza sativa L.)，INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH，2015年
21. Nontoxic virus nanofibers improve the detection sensitivity for the anti-p53 antibody, a biomarker in cancer patients，Nano Research ，2015年
22. Ultra Sensitive Voltammetric Determination of Hydrazine with Curcumin-Ordered Mesoporous Carbon Composite as Sensing Material，INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE，2015年
23. Ultrasensitive Rapid Detection of Human Serum Antibody Biomarkers by Biomarker-Capturing Viral Nanofibers，ACS NANO，2015年
24. Molecular and Biochemical Analysis of Chalcone Synthase from Freesia hybrid in Flavonoid Biosynthetic Pathway，PLoS ONE ，2015年
25. Formation of flowerlike gold nanostructure on ordered mesoporous carbon electrode and its application in electrochemical determination of ractopamine，MATER LETT，2015年
26. Detection of serum p53 antibodies from Chinese patients with papillary thyroid carcinoma using phage-SP-ELISA:correlation with clinical parameters，ENDOCRINE，2014年
27. Hybrid phage displaying SLAQVKYTSASSI induces protection against Candida albicans challenge in BALB/c mice，HUMAN VACCINES & IMMUNOTHERAPEUTICS，2014年
28. Tissue-Specific and Cation/Anion-Specific DNA Methylation Variations Occurred in C.virgata in Response to Salinity Stress，PLoS ONE ，2013年
29. Detection of Candida albicans Sap2 in cancer patient serum samples by an indirect competitive enzyme-linked immunosorbent assay for the diagnosis of candidiasis，INDIAN JOURNAL OF PATHOLOGY AND MICROBIOLOGY，2013年
30. Root-Specific DNA Methylation in Chloris virgata, a Natural Alkaline-Resistant Halophyte, in Response to Salt and Alkaline Stresses，PLANT MOL BIOL REP，2012年
31. Regeneration of Clivia miniata and assessment of clonal fidelity of plantlets，PLANT CELL TISS ORG，2012年
32. Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets，PLANT CELL REP，2012年
33. Methylation sensitive amplified polymorphism (MSAP) reveals that alkali stress triggers more DNA hypomethylation levels in cotton (Gossypium hirsutum L.) roots than salt stress，AFR J BIOTECHNOL，2011年
34. cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida，PLANT CELL REP，2011年
35. In Vitro Micropropagation of Freesia hybrida and the Assessment of Genetic and Epigenetic Stability in Regenerated Plantlets，J PLANT GROWTH REGUL，2010年
36. 红花香雪兰挥发油提取方法及化学成分分析，东北师大学报.自然科学版，2010年
37. 香雪兰花瓣总RNA的提取和cDNA文库的构建，东北师大学报（自然科学版），2008年
38. 穿龙薯蓣组织培养研究进展，东北师大学报(自然科学版）增刊，2007年
39. Volatile Compounds in the Flowers of Freesia Parental Species and Hybrids，JOURNAL OF INTEGRATIVE PLANT BIOLOGY，2007年
40. Specific Proteins in the Indirect Somatic Embryogenesis ofFreesia Refracta，CHEM RES CHINESE U，2007年
41. The Effect of Plant Growth regulators and Sucrose on the Micropropagation and Microtuberization of Dioscorea nipponica Makino，J PLANT GROWTH REGUL，2007年
42. 香雪兰基因组DNA快速提取方法，东北师大学报(自然科学版）增刊，2006年
专利：
正调控香雪兰花朵花色苷合成的MYB转录因子的cDNA序列 2018-10-09
香雪兰二氢黄酮醇-4还原酶基因的cDNA 2018-10-09
控制香雪兰花色苷合成的查尔酮异构酶的cDNA 2018-05-25
一种用于双表位展示的噬菌体载体及其构建方法 2018-05-15