Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 黄思铨教授 (肿瘤学系) |
| 陈德章教授 (肿瘤学系) |
| 欧志传先生 (肿瘤学系) |
| 林茵仪女士 (肿瘤学系) |
| 马碧如教授 (肿瘤学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1586/EPR.09.1 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/43WOS source URL
其它资讯
摘要Proteomic technologies have experienced major improvements in recent years. Such advances have facilitated the discovery of potential tumor markers with improved sensitivities and specificities for the diagnosis, prognosis and treatment monitoring of cancer patients. This review will focus on four state-of-the-art proteomic technologies, namely 2D difference gel electrophoresis, MALDI imaging mass spectrometry, electron transfer dissociation mass spectrometry and reverse-phase protein array. The major advancements these techniques have brought about and examples of their applications in cancer biomarker discovery will be presented in this review, so that readers can appreciate the immense progress in proteomic technologies from 1997 to 2008. Finally, a summary will be presented that discusses current hurdles faced by proteomic researchers, such as the wide dynamic range of protein abundance, standardization of protocols and validation of cancer biomarkers, and a 5-year view of potential solutions to such problems will be provided.
着者Wong SCC, Chan CML, Ma BBY, Lam MYY, Choi GCG, Au TCC, Chan ASK, Chan ATC
期刊名称EXPERT REVIEW OF PROTEOMICS
出版年份2009
月份4
日期1
卷号6
期次2
出版社Taylor & Francis: STM, Behavioural Science and Public Health Titles - No Open Select
页次123 - 134
国际标準期刊号1478-9450
电子国际标準期刊号1744-8387
语言英式英语
关键词2D difference gel electrophoresis; cancer biomarker; electron transfer dissociation; MALDI imaging mass spectrometry; proteomics; reverse-phase protein array
Web of Science 学科类别Biochemical Research Methods; BIOCHEMICAL RESEARCH METHODS; Biochemistry & Molecular Biology
