Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
| 温志昌教授 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1002/jcb.24617 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/13WOS source URL
其它资讯
摘要Although accumulating evidences indicate that miRNA emerge as a vital player in cell growth, development, and differentiation, how they contribute to the process of adipocyte differentiation remains elusive. In the present study, we revealed that the expression level of miR-210 was dramatically upregulated during 3T3-L1 adipogenesis. Ectopic introduction of miR-210 into 3T3-L1 cells promoted terminal differentiation as well as the expression of adipogenic markers. MTT assay showed that miR-210 significantly inhibited cell proliferation whereas the BrdU incorporation assay and flow cytometry analysis showed that miR-210 did not impair G1/S phase transition. Further experiments demonstrated that enhanced expression of miR-210 in 3T3-L1 cells provoked adipocyte differentiation via activation of PI3K/Akt pathway by targeting SHIP1, a negative regulator of PI3K/Akt pathway. Moreover, blockade of endogenous miR-210 during adipogenesis significantly repressed adipocyte differentiation. In summary, we have identified miR-210 as an important positive regulator in adipocyte differentiation through the activation of PI3K/Akt pathway. J. Cell. Biochem. 114: 2699-2707, 20http://aims.cuhk.edu.hk/converis/portal/Publication/13. (c) 20http://aims.cuhk.edu.hk/converis/portal/Publication/13 Wiley Periodicals, Inc.
着者Liang WC, Wang Y, Wan DCC, Yeung VSY, Waye MMY
期刊名称Journal of Cellular Biochemistry
详细描述To ORKTS: doi: 10.1002/jcb.24617
出版年份20http://aims.cuhk.edu.hk/converis/portal/Publication/13
月份12
日期1
卷号114
期次12
出版社Wiley: 12 months
页次2699 - 2707
国际标準期刊号0730-2312
电子国际标準期刊号1097-4644
语言英式英语
关键词3T3-L1; ADIPOGENESIS; MICRORNA; MIR-210
Web of Science 学科类别Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Cell Biology; CELL BIOLOGY
