Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
| 梁佩贤小姐 (生物医学学院) |
| 徐国荣教授 (生物医学学院) |
| 冯国培教授 (生物医学学院) |
| 詹兆?教授 (妇产科学系) |
| 梁佩贤小姐 (生物医学学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.bbrc.2010.08.064 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/18WOS source URL
其它资讯
摘要The relationship between DNA methylation, histone modifications and terminal differentiation in cardiomyocytes was investigated in this study. The upregulation of methylation-related proteins, including DNA methyltransferase (DNMT) 1, methyl-CpG binding domain proteins 1, 2 and 3, and the increase in global methylation during rat neonatal heart development were observed. Moreover, an increase in DNA synthesis and a delay in differentiation were found in 5-azacytidine (5-azaC)-treated cardiomyocytes. Increase in acetylation of H3-K9, H4-K5, H4-K8 and methylation of H3-K4 suggested a more accessible chromatin structure in 5-azaC-treated cells. Furthermore, methyl-CpG-binding protein 2 was found to be upregulated in differentiated cardiomyocytes. Overexpression of this protein resulted in an increase of global methylation levels. Therefore, we suggest that a hypermethylated genome and a more compact chromatin structure are formed during terminal differentiation of cardiomyocytes. (C) 2010 Elsevier Inc. All rights reserved.
着者Kou CYC, Lau SLY, Au KW, Leung PY, Chim SSC, Fung KP, Waye MMY, Tsui SKW
期刊名称Biochemical and Biophysical Research Communications
出版年份2010
月份9
日期17
卷号400
期次2
出版社Elsevier
页次278 - 283
国际标準期刊号0006-291X
电子国际标準期刊号1090-2104
语言英式英语
关键词5-Azacytidine; Acetylated histone; Chromatin structure; DNA methylation; Terminal differentiation
Web of Science 学科类别Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS
