Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 冯国培教授 (生物医学学院) |
| 陈月华博士 (那打素护理学院) |
| 邓铭权教授 (病理解剖及细胞学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.bbrc.2007.08.068 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/20WOS source URL
其它资讯
摘要Bioassay-guided phytochemical study of Androsace umbellata led to the successful isolation of saxifragifolin B (SB) for the first time. The anti-tumor effect of SB was firstly reported that it was shown to have potent cytotoxicity on human hepatoma HepG2 cells with IC50 value of 11.9 mu M at 24 h. Mechanistic studies were conducted, the accumulation of sub-G I population and the externalization of phosphatidylserine suggested that SB exerted its cytotoxic effect by induction of programmed cell death, which was confirmed by activation of PARP and caspase-3. Furthermore, SB-induced apoptosis on HepG2 cells was mediated by activation of caspase-8 and -9, mitochondrial membrane potential (Delta Psi(m).) collapse and the leakage of cytochrome c. In summary, this study provided evidence that SB isolated from A. umbellata could induce apoptosis on human hepatoma HepG2 cells and described the molecular mechanism. Our finding revealed the potential of SB as new chemotherapeutic agent for human hepatoma. (c) http://aims.cuhk.edu.hk/converis/portal/Publication/2007 Elsevier Inc. All rights reserved.
着者Zhang DM, Wang Y, Tang MK, Chan YW, Lam HM, Ye WC, Fung KP
期刊名称Biochemical and Biophysical Research Communications
出版年份http://aims.cuhk.edu.hk/converis/portal/Publication/2007
月份10
日期26
卷号362
期次3
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
页次759 - 765
国际标準期刊号0006-291X
电子国际标準期刊号1090-2104
语言英式英语
关键词Androsace umbellata; apoptosis; HepG2 cells; saxifragifolin B
Web of Science 学科类别Biochemistry & Molecular Biology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Biophysics; BIOPHYSICS
