Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 冯国培教授 (生物医学学院) |
| 陈月华博士 (那打素护理学院) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/j.canlet.2005.05.017 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/15WOS source URL
其它资讯
摘要Our previous study showed that arsenic trioxide (As2O3) was effective in inhibiting the growth of human hepatocellular carcinoma (HepG2) cells via induction of apoptosis. In the present study, we examined the effect of As2O3 on multidrug resistant human hepatocellular carcinoma (R-HepG2) cells which are characterized with overexpression of mdr1 gene and P-glycoprotein. The anti-proliferation of R-HepG2 by As2O3 was examined by MTT assay. For the induction of apoptosis, DNA fragmentation and Annexin V-PI staining were performed after treatment with arsenic trioxide. To study the effect of arsenic trioxide on P-glycoprotein, Western analysis probing anti-P-glycoprotein antibody was used to monitor the change of its expression. Results showed that As2O3 was effective in inhibiting the cell proliferation of R-HepG2 cells in a dose- and time-dependent manner via induction of apoptosis without affecting the cell cycle. The sensitivity of R-HepG2 cells toward As2O3 was found to be similar to that of the parental HepG2 cells. The Western analysis showed that As2O3 was probably not the substrate to be bound and extruded by P-glycoprotein in R-HepG2 cells because it could not maintain the cellular P-glycoprotein expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
着者Chan JYW, Siu KPY, Fung KP
期刊名称Cancer Letters
出版年份2006
月份5
日期18
卷号236
期次2
出版社ELSEVIER IRELAND LTD
页次250 - 258
国际标準期刊号0304-3835
电子国际标準期刊号1872-7980
语言英式英语
关键词apoptosis; arsenic trioxide; multidrug resistant; P-glycoprotein
Web of Science 学科类别Oncology; ONCOLOGY
