Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 韦妙宜教授 (那打素护理学院) |
| 徐国荣教授 (生物医学学院) |
| 李铭源博士 |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1016/S0014-4827(03)00018-1 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/10WOS source URL
其它资讯
摘要LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specific to the CDC25B2 isoform. Deletion and point mutation studies indicated that the second LIM domain of FHL3 was essential for this interaction. FRET experiments in C2C12 cells showed that, although both proteins were colocated in the cytoplasm and the nucleus, they interacted only in the nucleus. Finally, we showed that FHL3 binding impaired neither CDC25B2 phosphatase activity nor its localization. Further work is now needed to elucidate the consequences of this interaction on myoblast fate decision and cycle control. (C) 2003 Elsevier Science (USA). All rights reserved.
着者Mils V, Lee SMY, Joly W, Hang EWC, Baldin V, Waye MMY, Ducommun B, Kwok S, Tsui W
期刊名称Experimental Cell Research
出版年份2003
月份4
日期15
卷号285
期次1
出版社ACADEMIC PRESS INC ELSEVIER SCIENCE
页次99 - http://aims.cuhk.edu.hk/converis/portal/Publication/106
国际标準期刊号0014-4827
电子国际标準期刊号http://aims.cuhk.edu.hk/converis/portal/Publication/1090-2422
语言英式英语
关键词CDC25B; cell cycle; LIM-only protein
Web of Science 学科类别Cell Biology; CELL BIOLOGY; Oncology; ONCOLOGY
