Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 关海山教授 (生命科学学院) |
| 司徒莹影博士 |
| 李铭聪先生 (食品研究中心) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1128/EC.00222-07 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/2WOS source URL
其它资讯
摘要Endocytosis is the process by which substrates enter a cell without passing through the plasma membrane but rather invaginate the cell membrane and form intracellular vesicles. Rab7 regulates endocytic trafficking between early and late endosomes and between late endosomes and lysosomes. LeRab7 in Lentinula edodes is strongly homologous to Rab7 in Homo sapiens. Receptors for activated C kinase-1 (LeRACK1) and Rab5 GTPase (LeRAB5) were isolated as interacting partners of LeRab7, and the interactions were confirmed by in vivo and in vitro protein interaction assays. The three genes showed differential expression in the various developmental stages of the mushroom. In situ hybridization showed that the three transcripts were localized in regions of active growth, such as the outer region of trama cells, and the subhymenium of the hymenophore of mature fruiting bodies and the prehymenophore of young fruiting bodies. The existence of endocytosis in the mycelium and hymenophores was confirmed by the internalization of FM4-64. LeRAB7 was partially colocalized with the AM4-64 and was located in the late endocytic pathway. This is the first report of the presence of endocytosis in homobasidiomycetes. LeRAB7, LeRAB5, and LeRACK1 may contribute to the growth of L. edodes and cell differentiation in hymenophores.
着者Lee MT, Szeto CYY, Ng TP, Kwan HS
期刊名称Eukaryotic Cell
出版年份http://aims.cuhk.edu.hk/converis/portal/Publication/2007
月份1http://aims.cuhk.edu.hk/converis/portal/Publication/2
日期1
卷号6
期次1http://aims.cuhk.edu.hk/converis/portal/Publication/2
出版社AMER SOC MICROBIOLOGY
页次http://aims.cuhk.edu.hk/converis/portal/Publication/2406 - http://aims.cuhk.edu.hk/converis/portal/Publication/2418
国际标準期刊号1535-9778
语言英式英语
Web of Science 学科类别Microbiology; MICROBIOLOGY; Mycology
