Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 吕爱兰教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1038/cr.2015.143 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/12WOS source URL
其它资讯
摘要Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem cells (CSCs), are in clinical trials to investigate their ability to stimulate cardiac regeneration and repair. These studies were initially motivated by the purported cardiogenic activity of these cells. Recent lineage tracing studies using Kit promoter to drive expression of the inducible Cre recombinase showed that these CSCs had highly limited cardiogenic activity, inadequate to support efficient cardiac repair. Here we reassess the lineage tracing data by investigating the identity of cells immediately after Cre labeling. Our instant lineage tracing approach identifies Kit-expressing cardiomyocytes, which are labeled immediately after tamoxifen induction. In combination with long-term lineage tracing experiments, these data reveal that the large majority of long-term labeled cardiomyocytes are pre-existing Kit-expressing cardiomyocytes rather than cardiomyocytes formed de novo from CSCs. This study presents a new interpretation for the contribution of Kit(+) cells to cardiomyocytes and shows that Kit genetic lineage tracing over-estimates the cardiogenic activity of Kit(+) CSCs.
着者Liu QZ, Yang R, Huang XZ, Zhang H, He LJ, Zhang LB, Tian XY, Nie Y, Hu SS, Yan Y, Zhang L, Qiao ZY, Wang QD, Lui KO, Zhou B
期刊名称Cell Research
出版年份2016
月份1
日期1
卷号26
期次1
出版社INST BIOCHEMISTRY & CELL BIOLOGY
页次119 - 130
国际标準期刊号1001-0602
电子国际标準期刊号1748-7838
语言英式英语
关键词c-kit; cardiac stem cell; cardiomyocyte; lineage tracing
Web of Science 学科类别Cell Biology
