Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 张明仁教授 (妇产科学系) |
| 刘子建教授 (妇产科学系) |
| 梁子昂教授 (妇产科学系) |
| 卢煜明教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1086/302205 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/436WOS source URL
其它资讯
摘要Fetal DNA has been detected in maternal plasma during pregnancy. We investigated the clearance of circulating fetal DNA after delivery, using quantitative PCR analysis of the sex-determining region Y gene as a marker for male fetuses. We analyzed plasma samples from 12 women 1-42 d after delivery of male babies and found that circulating fetal DNA was undetectable by day 1 after delivery. To obtain a higher time-resolution picture of fetal DNA clearance, we performed serial sampling of eight women, which indicated that most women (seven) had undetectable levels of circulating fetal DNA by 2 h postpartum. The mean half-life for circulating fetal DNA was 16.3 min (range 4-30 min). Plasma nucleases were found to account for only part of the clearance of plasma fetal DNA, The rapid turnover of circulating DNA suggests that plasma DNA analysis may be less susceptible to false-positive results, which result from carryover from previous pregnancies, than is the detection of fetal cells in maternal blood; also, rapid turnover may be useful for the monitoring of fete-maternal events with rapid dynamics. These results also may have implications for the study of other types of nonhost DNA in plasma, such as circulating tumor-derived and graft-derived DNA in oncology and transplant patients, respectively.
着者Lo YMD, Zhang J, Leung TN, Lau TK, Chang AMZ, Hjelm NM
期刊名称American Journal of Human Genetics
出版年份1999
月份1
日期1
卷号64
期次1
出版社UNIV CHICAGO PRESS
页次218 - 224
国际标準期刊号0002-9297
电子国际标準期刊号1537-6605
语言英式英语
Web of Science 学科类别Genetics & Heredity; GENETICS & HEREDITY
