LIGHT-CHAIN RATIO OF SERUM IGA1 IN IGA NEPHROPATHY
Publication in refereed journal


香港中文大学研究人员 ( 现职)

黎嘉能教授 (内科及药物治疗学系)

林伟基教授 (化学病理学系)

全文

数位物件识别号 (DOI) http://dx.doi.org/10.1007/BF00917428

引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/11WOS source URL

其它资讯

摘要Patients with primary IgA nephropathy have deposits of IgA1 in their kidneys and increased IgA1 in circulation. We had previously shown that IgA nephritic patients displayed a unique immunological response characterized by a predominance of IgA with lambda chain in glomerular deposits and in circulation. We have now studied the kappa/lambda (k/lambda) ratio of serum IgA1 in 21 IgA nephritic patients at quiescence, with http://aims.cuhk.edu.hk/converis/portal/Publication/11 patients investigated during exacerbation as well. A novel enzyme-linked immunosorbent assay was used with monoclonal mouse anti-human IgA1 as the solid-phase capture antibody and peroxidase-labeled anti-human kappa and lambda antibodies as tracers. The ratio of serum IgA1 to total IgA (mean +/- SD) was significantly higher in patients (90.1 +/- 8.2% at quiescence, P < 0.01; 88.7 +/- 8.1% during exacerbation, P < 0.02) than in 20 healthy age- and sex-matched controls (80.0 +/- 9.8%). Furthermore, serum IgA1 kappa/lambda ratios were significantly lower in patients (1.02 +/- 0.27 at quiescence, P < 0.01; 0.93 +/- 0.16 during exacerbation, P < 0.01) than in controls (1.31 +/- 0.30). These findings indicate a predominance of lambda light-chain IgA1 in the serum of IgA nephritic patients. However, no difference in IgA1 kappa/lambda ratio was observed in these patients at quiescence and during exacerbation.

着者CHUI SH, LAM CWK, LEWIS WHP, LAI KN
期刊名称JOURNAL OF CLINICAL IMMUNOLOGY
出版年份1991
月份7
日期1
卷号http://aims.cuhk.edu.hk/converis/portal/Publication/11
期次4
出版社PLENUM PUBL CORP
页次219 - 223
国际标準期刊号0271-9142
语言英式英语

关键词ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA); IGA NEPHROPATHY; IGA1; KAPPA-LAMBDA RATIO
Web of Science 学科类别Immunology; IMMUNOLOGY