Optimization of fermentation medium for the new hydrogen bacterium E.Harbinense YUAN-3

ZHANG Lu-si^1,2, DING Jie¹, REN Nan-qi¹, GUO Wan-qian¹, GAO Lei¹, LIU Shuang³, ZHENG Guo-xiang^1,4

1.State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China;2.The Architectural Design and Research Institute,Harbin Institute of Technology,Harbin 150090,China;3.The Centre for Agricultural Engineering,Northeastern University,Harbin 150090,China;4.3.The Centre for Agricultural Engineering,Northeastern University,Harbin 150090,China



Abstract:

This study is aiming at investigating the metabolic characteristics of Ethanoligenens Harbinense YUAN-3.The effects of yeast extract powder and supplemental vitamins on the growth medium were assessed.With yeast powder as the sole nitrogen source,the biomass yield and hydrogen-producing capacity of YUAN-3 from glucose fermentation significantly increased.Adding vitamin in yeast powder can enhance hydrogen yield compared with no adding.Therefore vitamin supplement can be considered as an effective growth factor in this study.Batch culture also produced larger yields of hydrogen.

Key words:  biological hydrogen production  E.Harbinese YUAN-3  Novel strain  yeast  peptone

DOI：10.11916/j.issn.1005-9113.2011.02.027

Clc Number:Q93

Fund: