Measuring methods for three kinds of polyhydroxyalkanoates from activated sludge by gas chromatography
CHEN Wei1, CHEN Zhi-qiang1,2, LV Bing-nan1, WEN Qin-xue1, ZHANG Yun-hai1
1.State Key Lab of Urban Water Resource and Environment,School of Municipal and Environmental Engineering,Harbin Institute of Technology,Harbin 150090,China;2.National Engineering Research Center of Urban Water Resources,Harbin 150090,China
Abstract:
Gas chromatography determination of polyhydroxyalkanoates has been common;however,the pretreatment steps are often complex,and gas chromatography operation conditions are not given in detail.In this study,gas chromatography is used for analyzing PHB,PHV and PH2MV,three majors of PHAs in activated sludge.The sample was centrifuged at a speed of 4000 r/min for the separation of floc and supernatant,freezen,and dried for 12 h in vacuum freezing drier;and then transferred to the fridge for freezing to ice and drying for 12 h in vacuum freezing drier;then chloroform and a simple composition digestion solution including methanol,sulfuric acid and benzoic acid was added;digested at 105 ℃ for 6 h;cooled to room temperature,the lower solution of the result can be used for analyzing.Samples were analyzed by gas chromatography with FID detector and auto sampler;the standard curve of standard material shows an excellent linear relationship with correlation coefficients larger than 0.99;the relative standard deviation (RSD) of sludge samples is less than 1%.The recovery rates of each sample are between 95%-105%.The GC analysis time of each PHA sample is shorter than 10 minutes.
Key words: polyhydroxyalkanoates activated sludge gas chromatography measuring method
DOI：10.11916/j.issn.1005-9113.2011.05.020
Clc Number:X703.1
Fund:
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Measuring methods for three kinds of polyhydroxyalkanoates from activated sludge by gas chromatograp
本站小编 哈尔滨工业大学/2019-10-23
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