基本信息教育教学
研究领域
我们实验室的主要研究内容：

研究免疫与感染性疾病领域的重要生物大分子的结构和功能的关系。我们对膜上参与细胞信号转导的复合物以及重要病原如HIV与宿主相互作用的蛋白及其复合物的结构与功能的关系进行研究。

研究细菌CRISPR-Cas系统以及噬菌体Anti-CRISPR系统的分子机制，以及它们共进化“军备竞赛”的分子机制。同时基于研究成果靶向设计高效的、特异性的基因编辑工具。

病原感染引发的细胞死亡的分子机制研究。HIV等病原体感染导致细胞死亡，我们希望通过对受感染细胞发生死亡的机制进行研究找出参与该信号途径的重要分子和分子事件。

除了上述结构生物学及信号通路方面的基础研究外，我们也对免疫与感染性疾病发生过程中起关键作用的可溶/膜蛋白（复合物）的结构进行解析，并利用这些结构信息理性设计小分子药物治疗上述疾病。

我们采用整合的研究方法对感兴趣、且具有重要意义的课题进行研究，这一系列研究手段包括病毒学、结构生物学、分子细胞生物学以及小鼠遗传学等。将结构与体内外功能研究密切结合，从分子、细胞以及个体水平等多个层次，以较全视野研究目标蛋白质（复合物）分子结构和功能，及其信号调控机制。

招聘：该研究组招聘副教授、博士后、技术员

欢迎有大分子X射线晶体学、冷冻电镜、免疫学、噬菌体生物学、基因编辑等研究背景的人员申请。也欢迎有其它研究背景，如病原微生物学，细胞生物学，生物物理学或物理学博士学位，且对生命科学研究有强烈兴趣的人员申请。该实验室根据个人研究背景提供薪资待遇。请申请者将一份CV、以及三位推荐人的联系方式发至huangzhiwei@hit.edu.cn

发表文章:( ^* Co-first author, ^# Corresponding author）


Liyong Dong*, Xiaoyu Guan*, Ningning Li*, Fan Zhang, Yuwei Zhu, Kuan Ren, Ling Yu, Fengxia Zhou, Zhifu Han, Ning Gao & Zhiwei Huang^# (2019). An anti-CRISPR protein disables type V Cas12a by acetylation. Nature Structural & Molecular Biology. 26(4):308-314.

Minghui Guo*, Kaiming Zhang*, Yuwei Zhu, Grigore D. Pintilie, Xiaoyu Guan, Shanshan Li, Michael F. Schmid, Zhuo Ma, Wah Chiu^# & Zhiwei Huang^# (2019). Coupling of ssRNA Cleavage with DNase Activity in Type III-A CRISPR-Csm Revealed by Cryo-EM and Biochemistry. Cell Research. 29(4):305-312.

Minghui Guo*, Kuan Ren*, Yuwei Zhu*, Ziyun Tang, Yuhang Wang, Bailing Zhang & Zhiwei Huang^# (2019). Structural insights into a high fidelity variant of SpCas9. Cell Research. 29,183-192.

Yuwei Zhu, Zhiwei Huang^# (2018). Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools. National Science Review. doi: 10.1093/nsr/nwy150

Liuke Sun, Ruifang Shen, Sandeep K. Agnihotri, Yun Chen, Zhiwei Huang & Hansruedi Büeler^# (2018). Lack of PINK1 alters glia innate immune responses and enhances inflammation-induced, nitric oxide-mediated neuron death. Scientific Reports. 8(1):383.

Fan Zhang^#, Shijia Zhao, Chunyan Ren, Yuwei Zhu, Haibin Zhou, Yongkui Lai, Fengxia Zhou, Yuqiang Jia, Kangjie Zheng & Zhiwei Huang^# (2018). CRISPRminer is a knowledge base for exploring CRISPR-Cas systems in microbe and phage interactions. Communications Biology. 31;1:180.

De Dong*, Minghui Guo*, Sihan Wang*, Yuwei Zhu, Shuo Wang,Zhi Xiong, Jianzheng Yang, Zengliang Xu and Zhiwei Huang^# (2017). Structural basis of CRISPR-SpyCas9 inhibition by an anti-CRISPR protein. Nature. 546(7658):436-439.

Dan Wu*, Xiaoyu Guan*, Yuwei Zhu*, Kuan Ren, Zhiwei Huang^# (2017). Structural basis of stringent PAM recognition by CRISPR-C2c1 in complex with sgRNA.Cell Research. 27(5):705-708.

Marchisio^# & Zhiwei Huang^# (2017). CRISPR-Castype II-based Synthetic Biology applications in eukaryotic cells. RNA Biology. 14(10):1286-1293.

Pu Gao, Hui Yang, Kanagalaghatta R Rajashankar, Zhiwei Huang and Dinshaw J Patel (2016). Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition. Cell Research. 26(8):901-13.

De Dong*, Kuan Ren*, Xiaolin Qiu*, Jianlin Zheng, Minghui Guo, Xiaoyu Guan, Hongnan Liu, Ningning Li, Bailing Zhang, Daijun Yang, Chuang Ma, Shuo Wang, Dan Wu, Yunfeng Ma, Shilong Fan, Jiawei Wang, Ning Gao and Zhiwei Huang^# (2016). Crystal structure of CRISPR-Cpf1 in complex with CRISPR RNA (crRNA). Nature. 532(7600):522-6.

Yingying Guo*, Liyong Dong*, Xiaolin Qiu*, Yishu Wang, Bailing Zhang, Hongnan Liu, You Yu, Yi Zang, Maojun Yang and Zhiwei Huang^# (2014). Structural basis for hijacking CBF-β and CUL5 E3 ligase complex by HIV-1 Vif. Nature. 505(7482):229-33. (This paper is featured with News & Views, Nature. 505, 167-168, highlighted in Nature Structural & Molecular Biology, 21, 117 (2014))

Jae-Hyuck Shim, Matthew B. Greenblatt, Weiguo Zou, Zhiwei Huang, Marc N. Wein, Nicholas Brady, Dorothy Hu, Jean Charron, Heather R. Brodkin, Gregory A. Petsko, Dennis Zaller, Bo Zhai, Steven Gygi, Laurie H. Glimcher and Dallas C. Jones (2013). Schnurri-3 regulates ERK downstream of WNT signaling in osteoblasts. J Clin Invest. 123(9):4010-22.

Zehan Hu, Chuangye Yan, Peiyuan Liu, Zhiwei Huang, Rui Ma, Chenlu Zhang, Ruiyong Wang, Yueteng Zhang, Fabio Martinon, Di Miao, Haiteng Deng, Jiawei Wang, Junbiao Chang, Jijie Chai (2013). Crystal structure of NLRC4 reveals its autoinhibition mechanism. Science. 341(6142):172-5.

Weiguo Zou, Xi Chen, Jae Shim, Zhiwei Huang, Nicholas Brady, Dorothy Hu, Rebecca Drapp, Kirsten Sigrist, Laurie H. Glimcher, Dallas Jones (2011). The E3 ubiquitin ligase Wwp2 regulates craniofacial development through monoubiquitination of Goosecoid. Nature Cell Biology. 13(1):59-65.

Chen D, Lei L, Flores R, Zhiwei Huang, Wu Z, Chai J, Zhong G. (2010). Autoprocessing and self-activation of the secreted protease CPAF in Chlamydia-infected cells. Microbial Pathogene-sis. 49(4):164-73.

Zhiwei Huang and Jijie Chai (2010). Mapping the selection mechanisms by bacterial GEFs. Virulence. 1(2):93-6.

Zhiwei Huang*, Sarah E. Sutton*, Adam J. Wallenfang, Robert C. Orchard, Xiaojing Wu, Yingcai Feng, Jijie Chai and Neal M. Alto (2009). Structural insights into host GTPase isoform selection by a family of bacterial GEF mimics. Nature Structural & Molecular Biology. 16(8):853-60. (This publication was selected as cover story, and high-lighted by Nature China）

Zhiwei Huang, Yingcai Feng, Ding Chen, Xiaojing Wu, Xiaojun Wang, Xingguo Xiao, Wenhui Li, Niu Huang, Lichuan Gu, Guangming Zhong and Jijie Chai (2008). Structural basis for activation and inhibition of the secreted chlamydia protease CPAF. Cell Host & Microbe. 4(6):529-42. (This publication was selected as a research “highlight” by Nature China）

Maikke B. Ohlson, Zhiwei Huang, Neal M. Alto, Jack E. Dixon, Jijie Chai and Samuel I. Miller (2008). Structure and Function of Salmonella SifA Indicate that Its Interactions with SKIP, SseJ, and RhoA Family GTPases Induce Endosomal Tubulation. Cell Host & Microbe. 4(5):434-46.


工作经历
时间工作经历
2012-Present哈尔滨工业大学生命科学与技术学院，教授、博士生导师
2009-2012哈佛大学免疫与感染疾病系，博士后




教育经历
2004-2008 北京生命科学研究所生化与分子生物学博士

2003-2004 中国农业大学生化与分子生物学博士（博士课程学习）

1999-2003 兰州理工大学化学工程与工艺学士

主要任职
哈工大生命科学与技术学院教授、院长

哈工大生命科学中心主任

荣誉称号
国家杰出青年科学基金

谈家桢生命科学创新奖

中国青年科技奖

教育部“****奖励计划”青年项目

国家优秀青年科学基金

黑龙江省杰出青年科学基金


讲授课程
结构分子生物学

简介:

生命科学研究进展

简介:

招生信息
硕士、博士招生:

欢迎有大分子X射线晶体学、冷冻电镜、HIV病毒学、免疫学、噬菌体生物学、基因编辑等研究背景的人员申请。也欢迎有其它研究背景，如病原微生物学，细胞生物学，生物物理学或物理学学位且对生命科学研究有强烈兴趣的人员申请。