一、基本信息
陈纯琪，博士，教授，博士生导师
电子邮件：ccckate0722@hubu.edu.cn
研究方向：免疫学、微生物学、结构生物学与酶学
研究领域：结构生物学、酶应用与改造、酶的机理研究。酶是所有生命活动的发动机，酶的结构与催化机理研究对于了解生物基本运作、疾病发生原因、药物开发、以及医药产业与绿色生物工艺发展具有重大意义。陈纯琪教授通过结构生物学方法(X光晶体学与冷冻电镜)解析酶的三维结构与底物结合的复合体结构，阐释催化机理，并开展基于结构的理性设计，改造酶的特征与活性。工作内容主要涵盖三个体系：(1)新型萜类合成酶；(2)天然与人造合成塑料降解酶；(3)霉菌毒素分解酶。目前已在Nature Reviews Chemistry, Immunity, Nature Communications, Angew. Chem. Intl. Ed., JACS, ACS Catalysis等SCI期刊发表论文70余篇，共20篇获选为封面文章，申请国内外专利24个，已授权16个。2019年获天津市自然科学二等奖（第二完成人）
二、教育背景
2018.06至今 湖北大学生命科学学院，教授
2014.01–2018.5 中国科学院天津工业生物技术研究所，访问****，高级台湾青年访问****
三、工作经历
2012.01–2013.12 中国科学院微生物研究所，副研究员，高级台湾青年访问****
2008.02–2011.12 台湾中研院特聘博士后
2003.09–2008.01 台湾大学，微生物学研究所，博士
1999.09–2001.07 台湾大学，医事技术学研究所，硕士
1995.09–1999.07 台湾大学，医事技术学系，学士
四、科研项目
1. 国家自然科学基金，面上项目（**）：新型倍半萜合成酶晶体结构与催化机理研究，2020.01–2023.12，58万，在研，主持
2. 国家自然科学基金青年基金（**）：免疫球蛋白样转物3受体鉴定：功能性以及结构性研究，2013.01–2015.12，25万，已结题，主持
五、发表论文（^#共同一作，*通讯作者）
1. Chen CC^#, Yu X^#, Kuo CJ^#, Min J, Chen S, Ma L,* Liu K,* Guo RT* (2021) Overview of antiviral drug candidates targeting coronaviral 3C-like main proteases. FEBS Journal doi.org/10.1111/febs.15696 (Review)
2. Chen CC, Min J, Zhang L, Yang Y, Yu X,* Guo RT*. (2021) Advanced understanding of the electron transfer pathway of cytochrome P450s ChemBioChem 22, 1317–1328.
3. Chen CC^#, Malwal SR^#, Han X^#, Liu W, Ma L, Zhai C, Dai L, Huang JW, Shillo A, Desai J, Ma X, Zhang Y, Guo RT*, Oldfield E*. (2021) terpene cyclases and prenyltransferases: structures and mechanisms of action. ACS Catalysis 11, 290–303 (Cover story)
4. Zhang L, Xie Z, Liu Z, Zhou S, Ma L, Liu W, Huang JW, Ko TP, Li X, Hu Y, Min J, Yu X, Guo RT*, Chen CC*. (2020) Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Nature Communications 11, 2676 (Highlighted by C&EN, ChemistryViews,科技日报,中国科学报)
5. Chen CC,* Zhang L, Yu X, Ma L, Ko TP, Guo RT* (2020) Versatile cis-isoprenyl diphosphate synthase superfamily members in catalyzing carbon-carbon bond formation. ACS Catalysis 10, 4717–4725. (Review, ACS Editors’ Choice, Cover story)
6. Chen CC, Dai L, Ma L*, Guo RT* (2020) Enzymatic degradation of plant biomass and synthetic polymers. Nature Reviews Chemistry 4, 114–126. (Review, Cover story)
7. Yang Y, Li L, Yuan L, Zhou X, Duan J, Xiao H, Cai N, Han S, Ma X, Liu W, Chen CC, Wang L, Li X, Chen J, Kang N, Chen J, Shen, Z, Malwal, S R, Liu W, Shi Y, Oldfield E, Guo RT, Zhang Y. (2019) A Structural change in butyrophilin upon phosphoantigen binding underlies phosphoantigen-mediated Vgamma9Vdelta2 T cell activation. Immunity 50, 1043–1053.
8. Sun H, Ko T P, Liu W, Liu W, Zheng Y, Chen CC*, Guo RT*. (2019) Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate. Biochemical and Biophysical Research Communications 521, 31–36.
9. Kuo CJ, Gao J, Huang JW, Ko TP, Zhai C, Ma L, Liu W, Dai L, Chang YF, Chen TH, Hu Y, Yu X, Guo RT,* Chen CC*. (2019) Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis. Biochimica et Biophysica Acta General subjects 1863, 1351–1359.
10. Tang X^#, Xue J^#, Yang Y, Ko T P, Chen CY, Dai L, Guo RT , Zhang Y*, Chen CC*. (2019) Structural insights into the calcium dependence of Stig cyclases. RSC Advances 9, 13182.
11. Ma J, Ko T P, Yu X, Zhang L, Ma L, Zhai C, Guo RT, Liu W, Li H, Chen CC*. (2019) Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1. Biochemical and Biophysical Research Communications 515, 621–626.
12. Zhang L, Ko TP, Malwal SR, Liu W, Zhou S, Oldfield E, Guo RT*, Chen C C*. (2019) Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism. Biochemical and Biophysical Research Communications 511, 800–805.
13. Chang Z, Ansbacher T, Zhang L, Yang Y, Ko TP, Zhang G, Liu W, Huang JW, Dai L, Guo RT, Major DT*, Chen CC*. (2019) Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis. Organic Biomolecular Chemistry17, 2070–2076. (Cover story)
14. Chen CC^#, Hu X^#, Tang X^#, Yang Y^#, Ko TP, Gao J, Zheng Y, Huang JW, Yu Z, Li L, Han S, Cai N, Zhang Y,* Liu W,* Guo RT*. (2018) The crystal structure of a class of cyclases that catalyze the cope rearrangement. Angewandte Chemie International Edition 57, 15060–15064. (Cover story)
15. Chen CC^#, Han X^#, Ko TP, Liu W*, Guo RT*. (2018) Structural studies reveal the molecular mechanism of PETase. FEBS Journal 285, 3717–3723.
16. Zheng Y^#, Liu W^#, Chen CC^#, Hu X, Liu W, Ko TP, Tang X, Wei H, Huang JW, Guo RT*. (2018Crystal structure of a mycoestrogen-detoxifying lactonase from Rhinocladiella mackenziei: molecular insight into ZHD substrate selectivity. ACS Catalysis 8, 4294–4298.
17. Han, X^#, Liu W^#, Huang JW^#, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT*. (2017) Structural insight into catalytic mechanism of PET hydrolase. Nature Communications13, 2106.
18. Zhang L^#, Chen CC^#, Ko TP^#, Huang JW, Zheng Y, Liu W, Wang I, Malwal SR, Feng X, Wang Ke, Huang CC, Hsu ST, Wang AH, Oldfield E*, Guo R T*. (2016) Moenomycin biosynthesis: structure and mechanism of action of the prenyl transferase MoeN5. Angewandte Chemie International Edition 55, 4716–4720.
19. Xu Z^#, Liu W^#, Chen CC^#, Li Q, Huang JW, Ko TP, Liu G, Liu W, Peng W, Cheng YS, Chen Y, Jin J, Li H,* Zheng Y,* Guo RT*. (2016) Enhanced α-zearalenol hydrolyzing activity of a mycoestrogen detoxifying lactonase by structure-based engineering. ACS Catalysis 6, 7657–7663. (Cover story)
20. Liu M^#, Chen CC^#, Chen Lu^#, Xiao X, Zheng Y, Huang JW, Liu W, Ko TP, Cheng YS, Feng X, Oldfield E*, Guo RT*, Ma Y*. (2016) Structure and function of a “head-to-middle” prenyltransferase: lavandulyl diphosphate synthase. Angewandte Chemie International Edition 55, 4721–4724. (Cover story)
21. Huang JW^#, Liu W^#, Lai H L, Cheng YS, Zheng Y, Li Q, Sun H, Kuo C J, Guo RT*, Chen CC*. (2016) Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50. Biochemical and Biophysical Research Communications 16, 565–572.
22. Yang Y^#, Ko TP^#, Chen CC^#, Huang G, Zheng Y, Liu W, Wang I, Ho M, Hsu S, O'Dowd B, Huff H, Huang C H, Docampo R, Oldfield E,* Guo RT*. (2016 Structures of trypanosome vacuolar soluble pyrophosphatases: anti-parasitic drug targets. ACS Chemical Biology 11, 1362–1371
23. Haywood J, Qi J, Chen, CC, Lu G, Liu Y, Yan J, Shi Y, Gao GF. (2016 Structural basis of collagen recognition by human osteoclast-associated receptor and design of osteoclastogenesis inhibitors. Proceedings of the National Academy of Sciences of the United States of America 13, 1038–1043.
24. Cheng YS^#, Chen CC^#, Huang JW, Ko TP, Huang Z, Guo RT*. (2015) Improving the catalytic performance of a GH11 xylanase by rational protein engineering. Applied Microbiology and Biotechnology 99, 9503–9510.
25. Zheng Y^#, Chen C C^#, Ko TP, Xiao X, Yang Y, Huang CH, Qian G, Shao W*, Guo RT*. (2015) Crystal structures of S-adenosylhomocysteine hydrolase from the thermophilic bacteriumThermotoga maritima. Journal of Structural Biology 190, 135–142. (Cover story)
26. Chen CC, Cheng KJ, Ko TP, Guo RT*. (2015) Current progresses in phytase research: three-dimensional structure and protein engineering. ChemBioEng Reviews 2, 76–86. (Review, Cover story)
27. Chen CC, Ko TP, Huang JW, Guo RT*. (2015) Heat- and alkaline-stable xylanases: applications, protein structure and engineering. ChemBioEng Reviews 2, 95–106. (Review)
28. Chen CC^#, Huang JW^#, Zhao P, Ko TP, Huang CH, Chan HC, Huang Z, Liu W, Cheng YS, Liu JR, Guo, RT*. (2015) Structural analyses and yeast production of the β-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum. Enzyme and Microbial Technology 71, 1–7.
29. Han X^#, Chen CC^#, Kuo CJ, Huang CH, Zheng Y, Ko TP, Zhu Z, Feng X, Wang K, Oldfield, E, Wang AH, Liang PH, Guo RT*, Ma Y*. (2015) Crystal structures of ligand-bound octaprenyl pyrophosphate synthase from Escherichia coli reveal the catalytic and chain-length determining mechanisms. Proteins 83, 37–45.
30. Cheng YS^#, Chen CC^#, Huang CH, Ko TP, Luo W, Huang JW, Liu J R, Guo RT*. (2014) Structural analysis of a glycoside hydrolase family 11 xylanase from Neocallimastix patriciarum: insights into the molecular basis of a thermophilic enzyme. The Journal of Biological Chemistry 289, 11020–11028.
31. Wu TH^#, Chen CC^#, Cheng YS, Ko TP, Lin CY, Lai HL, Huang TY, Liu JR*, Guo RT*. (2014) Improving specific activity and thermostability of Escherichia coli phytase by structure-based rational design. Journal of Biotechnology 175, 1–6.
32. Huang JW^#, Chen CC^#, Huang CH, Huang TY, Wu TH, Cheng YS, Ko TP, Lin CY, Liu J R*, Guo RT*. (2014) Improving the specific activity of beta-mannanase from Aspergillus niger BK01 by structure-based rational design. Biochimica et Biophysica Acta 1844, 663–669. (Cover story)
33. Chen CC^#, Luo H^#, Han X^#, Lv P, Ko TP, Peng W, Huang CH, Wang K, Gao J, Zheng Y, Yang Y, Zhang J, Yao B,* Guo RT*. (2014) Structural perspectives of an engineered beta-1,4-xylanase with enhanced thermostability. Journal of Biotechnology 189, 175–182.
34. Lin C W, Chang Y L, Chang YC, Lin J C, Chen CC, Pan SH, Wu CT, Chen HY, Yang SC, Hong TM, Yang PC. (2013) MicroRNA-135b promotes lung cancer metastasis by regulating multiple targets in the Hippo pathway and LZTS1. Nature communications 4, 1877.
35. Chen CC, Chang CM, Sun CP, Yu CP, Wu PY, Jeng KS, Hu CP, Chen PJ, Wu JC, Shih, CH, Gershwin ME, Tao MH. (2012) Use of RNA interference to modulate liver adenoma development in a murine model transgenic for hepatitis B virus. Gene therapy 19, 25–33
36. Chen CC, Sun CP, Ma HI, Fang CC, Wu PY, Xiao X, Tao MH. (2009) Comparative study of anti-hepatitis B virus RNA interference by double-stranded adeno-associated virus serotypes 7, 8, and 9. Molecular therapy 17, 352–359.
37. Chen CC, Ko TM, Ma HI, Wu HL, Xiao X, Li J, Chang CM, Wu PY, Chen C H, Han JM, Yu CP, Jeng KS, Hu CP, Tao MH. (2007) Long-term inhibition of hepatitis B virus in transgenic mice by double-stranded adeno-associated virus 8-delivered short hairpin RNA. Gene therapy 14, 11–19.
六、已授权专利
1. 具有提升的酶活性的植酸酶，中国，CNB，2/8
2. 具有提升的酶活性的植酸酶，中国，CNB，2/8
3. Cellulase having improved enzymatic activity，美国，US**B1，2/8
4. Phytase having improved thermostability，美国，US**B2，7/8
5. Glucose oxidase having improved thermostability，美国，US**B2，9/10
6. Lactone hydrolase and method of degrading alpha–zearalenol using the same，美国，US**B2，4/8
7. Lysozyme having improved enzymatic activity，美国，US**B2，1/4
8. Xylosidase having improved enzymatic activity，美国，USA1，9/10
9. Cellulase having improved enzymatic activity，美国，US**，1/11
七、获奖与荣誉称号
1. 2020年，湖北省创新群体
2. 2020年，湖北省****特聘专家
3. 2020年，湖北省楚天********
4. 2019年，天津市自然科学二等奖（第二完成人）