一、基本信息
王行国，教授、博士生导师
办公电话：-8066
电子邮件：xgwang@hubu.edu.cn
社会兼职：湖北省生物物理学会常务理事、湖北省微生物学会理事、湖北省生物工程学会理事
研究领域：微生物生化与分子生物学、分子酶学、肿瘤靶向治疗。
二、教育背景
1978.03–1982.01 华中师范大学生物系，理学学士
1985.09–1988.07 华中师范大学生物系，理学硕士
1993.09–1996.07 英国谢菲尔德大学分子生物学与生物技术系，博士
三、工作经历
2004.04至今湖北大学生命科学学院，教授
1997.10–2004.03 美国爱因斯坦医学院和塔芙斯大学，研究员
1996.08–1997.09 爱尔兰都柏林生化系，博士后
四、科研项目
1. 湖北省科技厅项目：TRAIL-同位素偶联制备多功能杀癌靶向药物的研究
2. 教育部博士点基金：磷脂酰胆碱在植物细菌致病性中的作用与机理研究
3. 国家自然科学基金：磷脂酰胆碱合成酶基因在细菌致病性中的作用与机理
4. 国家留学人员科技活动重点项目: 转氨酶工程菌催化非天然氨基酸合成的研究
5. 武汉市科技局对外科技合作与交流项目: 构建转氨酶工程菌生产L–型非天然氨基酸的研究
6. 国家自然科学基金：细菌磷脂酰胆碱生物学功能的研究
7. 国家科技部国际合作处中爱政府间科技合作项目：转氨酶催化生产非天然氨基酸的研究
8. 国家人事部留学回国人员基金：细菌磷脂酰胆碱的生物学功能
9. 湖北省自然科学基金：不同性质的细菌漆酶菌株的筛选、基因克隆、表达及酶学分析
10. 湖北省教育厅项目：细菌铜离子结合蛋白的漆酶活性的研究
11. 武汉市科技局项目：细菌铜离子结合蛋白与细菌漆酶活性
12. 武汉高新第五批“3551光谷人材计划”：胶乳增强研发生产平台。
五、代表性论文
1. Long D, He H, Xiong M, Li Y, Wu W, Wang XG (2013) A soil-dwelling Pseudomonas sp.593 harboring an exogenous hrpZ gene elicits a typical hypersensitive response in tobacco and soybean leaves. International Journal of Microbiology Research 5, 374–380.
2. Yu X, Bresser J, Schall I, Djurdjevic I, Buckel W, Wang XG, Engel PC. (2012) Development of a satisfactory and general continuous assay for aminotransferases by coupling with (R)-2-hydroxyglutarate dehydrogenase. Analytical Biochemistry 431, 127–131.
3. Li Y, Zuo W, Li Y, Wang XG （2012）Cloning of multicopper oxidase gene from Ochrobactrum sp. 531 and characterization of its alkaline laccase activities towards phenolic substrates. Advances in Biological Chemistry. 2, 248–255.
4. He H, Wu B, Xiong M, Li Y, Wenhua Wu, Wang X (2011) Gene cloning, structural gene and promoter identification, and active assay of the phosphatidylcholine synthase of Pseudomonas sp. strain 593. Canadian Journal of Microbiology 57, 785–794.
5. Chen F, Lv M, Xue Y, Zhou J, Hu F, Chen X, Zhao Z, Li Y, Wang X. (2011) Genetic variants of fibroblast growth factor receptor 2 (FGFR2) are associated with breast cancer risk in Chinese women of the Han nationality. Immunogenetics 64,71–76 .
6. Li Y, Gong Z, Li X, Li Y, Wang XG. (2011) Engineering Klebsiella sp. 601 multicopper oxidase enhances the catalytic efficiency towards phenolic substrates. BMC Biochemistry 12, 30.
7. Harris M, Wang XG, Jiang Z, Goldberg GL, Casadevall A, Dadachova E. (2011) Radioimmunotherapy of experimental head and neck squamous cell carcinoma (HNSCC) with E6-specific antibody using a novel HPV-16 positive HNSCC cell line. Head & Neck Oncology 3, 9.
8. Wang XG, Phang P, Susan Lynch S& Paul Engel P (2010) Forced evolution of Escherichia coli cells with the ability to effectively utilize non-natural amino acids L-tert-leucine, L-norleucine and γ-methyl-L-leucine. Biocatalysis and Biotransformation, 28, 293–303.
9. Phaeto R, Harris M, Jiang Z, Wang XG, Mark H. Einstein MH, Goldberg GL, Casadevall A, Dadachova E. (2010) Radioimmunotherapy with antibody to HPV16 E6 oncoprotein is effective in experimental cervical tumor expressing low levels of E6. Cancer Biology & Therapy 10, 1041–1047.
10. Phaeton B, Wang XG, Einstein MH, Goldberg GL, Casadevall A, Dadachova E. (2010) The influence of proteasome inhibitor MG132, external radiation, and unlabeled antibody on the tumor uptake and biodistribution of ¹⁸⁸Re-labeled anti-E6 C1P5 antibody in cervical cancer in mice. Cancer 116,1067–1074.
11. Chen F, hao Q, Cai X, Lv L, Wen Lin W, Xuejin Yu X, Li C, Yang Li Y, Min Xiong M, Wang XG. (2009) Phosphatidylcholine in membrane of Escherichia coli changes bacterial antigenicity. Canadian Journal of Microbiology 55,1328–1334.
12. Li Y, Yin J, Qu G, Lv L, Li Y, Yang S, Wang XG. (2008) Gene cloning, protein purification and enzymatic properties of multicopper oxidase from Klebsiella sp. 601. Canadian Journal of Microbiology 54, 725–733.
13. Wang XG, Revskaya E, Bryan RA, Strickler HD, Burk RD, Casadevall A, Dadachova E. (2007) Treating cancer as an infectious disease-viral antigens as novel targets for treatment and potential prevention of tumors of viral etiology. PLoS ONE 2, e1114.
14. Dadachova E, Wang XG, Casadevall A. (2007) Targeting the virus with radioimmunotherapy in virus-associated cancers. Cancer Biotherapy& Radiopharmaceuticals 22, 303–308.
15. Medrano MS, Ding Y, Wang XG, Lu P, Coburn J, Hu LT. (2007) Regulations of expression of the oligopeptide permease a proteins of Borrelia burgdorferi. Journal of Bacteriology 189, 2653–2659.
16. Wang XG, Scagliotti JP, Hu LT (2004) Phospholipid synthesis in Borrelia burgdorferi, BB0249 and BB0721 encod functional phosphatidylcholine synthase and phosphatidylglycerolphosphate synthase proteins. Microbiology 150, 391–397.
17. Wang XG, Kidder M, Scagliotti JP, Klempner MS, Noring R, Hu LT. (2004) Analysis of differences in the functional properties of the substrate binding proteins of the Borrelia burgdorferi oligopeptide permease (opp) operon. Journal of Bacteriology 186, 51–60.
18. Wang XG, Michael Kidder M, Telford S, Hu LT. (2002) Effects of environmental Changes on expression of the oligopeptide permease (opp) genes of Borrelia burgdorferi. Journal of Bacteriology 184, 6198–6206.
19. Wang XG, Olsen LR, Roderick SL. (2002) Structure of the lac operon galactoside acetyltransferase. Structure 10, 581–588.
20. Wang XG, Britton KL, Timothy J. Stillman TJ, Rice DW, Engel PC. (2001) Conversion of a glutamate dehydrogenase into methionine/norleucine dehydrogenase by site-directed mutagenesis. European Journal of Biochemistry 268, 5791–5799.
六、受权专利
1. 王行国：活体催化工程菌及利用??酮酸生产L–型非天然疏水性氨基酸的方法（专利号ZL9.9）
2. 王行国 李洋 龚子君 左文峰：在大肠杆菌中高效表达可溶性细菌漆酶的方法（专利号：ZL5.6）
3. 王行国 叶青 陈凡: 可溶性截短的人TRAIL活性蛋白的制备方法 (专利号ZL 9.6)
七、获奖荣誉
1. 湖北省科协“科技创新源泉工程” 创新创业人才奖 (2012)
2. 第四届中国侨联创新人才奖(2012)
3. 国务院政府特殊津贴 (2008)