一、基本信息
李顺意，博士，副教授、硕士研究生导师
办公电话：-8040
电子邮件：lisy@hubu.edu.cn
社会兼职：湖北省生化与分子学会理事、湖北省生物物理学会理事
研究领域：生物化学与分子生物学、蛋白质化学
二、教育背景
1991.09–1994.07 湖南师范大学生物学系，理学硕士
1994.09–1997.07 中国科学院昆明动物研究所，理学博士
三、工作经历
1997.08至今 湖北大学生命科学学院讲师、副教授
2004.02–2008.02 美国佐治亚州立大学化学系，博士后
四、科研项目：
1. 钙离子与蛇毒磷脂酶A2的结构和功能的关系研究，教育部留学人员回国人员基金，2011-2013年，主持
2. 湖北省自然科学基金重点项目（2010CDB04507，2010），主持
3. 湖北省教育厅自然科学基金（DC**，2010），主持
五、主要论文
1. Li S, Jiang J, Zou J, Qiao J, Xue S, Wei L, Long R, Wang L, Castiblanco A, Maor N, Ngo J, Mao H, Liu L, Yang JJ. (2012) PEGylation of protein-based MRI contrast agents improves relaxivities and biocompatibilities. Journal of Inorganic Biochemistry 107, 111–118.
2. Zhang G, Li S, et al. (2012) Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10. Extremophiles 16, 35–42.
3. Qiao J, Li S, Jiang J, Wei L, Liang R, Wang L, Mao H, Yang H. (2011) Grossniklaus H, Liu Z, Yang JJ. (2011) HER-2 targeted molecular MR imaging using a de novo designed protein contrast agent. PLoS One 6, e18103.
4. Wei L, Li S, Yang, Ye, Y, Zou J, Wang, L, Long, R, Zurkiya O, Zhao T, Johnson J, Qiao J, Zhou W, Castiblanco A, Maor N, Chen Y, Mao H, Hu X, Yang JJ, Liu ZR. (2010) Protein-based MRI contrast agents for molecular imaging of prostate cancer. Molecular Imaging and Biology 13, 416-423.
5. Li S, Yang W, Maniccia A, Barrow D, Jr Tjong, H, Zhou H, Yang J. (2008) Rational design of a Ca²⁺-binding protein with switchable conformation. FEBS Journal 275, 5048-5061.
6. Chen N, Ye Y, Zou, J, Li S, Wang S, Martin A, Wohlhueter R, Yang J. (2009) Fluorescence complementation via EF-hand interactions. Journal of Biotechnology 142, 205–213.
7. Zhou Y, Tzeng W, Ye Y, Huang Y, Li S, Chen Y, Frey T, Yang J. (2009) A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication. Biochemical Journal 417, 477–483.
8. Maniccia A, Yang W, Johnson J, Li S, Tjong H, Zhou H, Shaket L, Yang J. (2009) Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteins. PMC Biophysics 2, 11.
9. Yang J, Yang J, Wei L, Zurkiya, O, Yang W, Li S, Zou J, Zhou Y, Maniccia A, Mao H, Zhao F, Malchow R, Zhao S, Johnson J, Hu X, Krogstad E, Liu Z. (2008) Rational design protein based MRI contrast agents with high relaxivity Journal of the American Chemical Society 130, 9260–9267.
10. Huang Y, Zhou Y, Yang W, Butters R, Lee H, Li S, Castiblanco A, Brown E, Yang J. (2007) Identification and dissection of Ca²⁺-binding sites in the extracellular domain of Ca²⁺-sensing receptor. Journal of Biological Chemistry 282, 19000–19010.
11. Maniccia A, Yang W, Li S, Johnson J, Yang J. (2006) Using protein design to dissect the effect of charged residues on metal binding and protein stability. Biochemistry 45, 5848–5856.
12. Yang W, Wilkins A, Li S, Ye Y, Yang J. (2005) The effects of Ca²⁺ binding on the dynamic properties of a designed Ca²⁺–binding protein. Biochemistry 44, 8267–8273.
13. Yang W, Wilkins A, Ye Y, Liu Z, Li S, Urbauer J, Hellinga H, Kearney A, van der Merwe P, Yang J. (2005) Design of a calcium-binding protein with desired structure in a cell adhesion molecule. Journal of the American Chemical Society 127, 2085–2093.