姓名： 李桂银
正高级 女 生物与环境科学学院 邮箱:liguiyin01@163.com





研究领域生物医学传感;生物纳米材料;靶向纳米探针;
个人简介


李桂银，女，1972年生，博士/博士后，生命与环境科学学院教授，博士生导师。2008年毕业于中南大学化学化工学院，获生物化工博士学位。2008年进入中南大学药学博士后流动站从事博士后研究，2010年9月优秀出站进入桂林电子科技大学生命与环境科学学院从事教学与科研工作。主要研究方向为生物医学传感与智能仪器，靶向分子探针，生物纳米材料。2014年为桂林电子科技大学生物医学工程专业学术带头人， 2008年入选湖南省普通高校中青年骨干教师培养对象，2009年获得湖南省“芙蓉百岗明星”称号。获广西科技技术发明奖三等奖1项（排名第二），广西科技技术进步奖三等奖1项（排名第二），广西医药卫生适宜技术推广奖二等奖1项（排名第二）。近5年来，主持国家自然科学基金项目2项，中国博士后基金2项，广西科技厅项目3项等多项省部级科研项目。已在国内外知名杂志上发表SCI收录论文50多篇，其中以第一或者通讯作者SCI收录论文30篇；申请国家发明专利46项，以第一完成人授权12项。



教育背景


2001-2008年在中南大学化学化工学院分别获得硕士和博士学位



工作经历


2010/10-今，桂林电子科技大学，生命与环境科学学院，教授



2015/06-2016/07， University of Michigan, Biomedical Engineering, Visiting Scholar



2014/01-2018/12，广西医科大学生物靶向诊治中心，临床医学博士后2013/01-2014/01, 桂林电子科技大学，生命与环境科学学院副院长



2008/11-2010/9，中南大学药学院，药学博士后流动站，药学博士后



2000/01-2001/08，湖南金正方实业有限公司，技术员，工程师



1995/07-1999/12，湖南丽臣实业有限公司，检验员



主要荣誉

曾获得奖励



1） 李桂银，广西医药卫生适宜技术推广奖二等奖（排名第二），基于哑铃型Au-Fe3O4纳米材料的生物传感方法构建及其临床应用，省级，2019年



2） 李桂银，广西科技技术进步奖三等奖（排名第二），基于磁纳米金的生物传感方法构建及其临床应用, 省级，2019年



3） 李桂银，广西科技技术发明奖三等奖（排名第二），柿单宁提取制粉技术及功能化应用，省级，2018年



4） 李桂银，桂林电子科技大学生物医学专业学术带头人，校级，2014年



5） 李桂银，广西高等教育自治区教学成果奖（第八），广西高等教育自治区教学成果奖，校级，2012年



6） 李桂银，桂林电子科技大学学术骨干，校级，2011年



7） 李桂银，湖南省“芙蓉百岗明星”，湖南省总工会，省级，2009年



8） 李桂银，湖南省普通高校中青年骨干教师，湖南教育厅，省级，2008年










学术活动










教学信息


《药理学（英文教材）》、《药剂学》、《酶工程》、《药学概论》、《药物化学》、《医学检验原理与技术》、《生物材料》、《生物力学》、《分子生物学》、《微生物与免疫学》、《生物信息学数据挖掘》等



部分论文(*通讯作者)

[1] ZhongminWang, MingminGao, XiaojuanLi, JinliangNing, ZhideZhou*, Guiyin*. Efficient adsorption of methylene blue from aqueous solution by graphene oxide modified persimmon tannins. Materials Science & Engineering C-Materials for Biological Applications, 108(2020) 11096 (SCI, IF=5.07，2区)



[2] Guiyin Li, Junxiang Zeng,Le Zhao, Zhihong Wang, Chenyang Dong, Jintao Liang*, Zhide Zhou*, Yong Huang*.Amperometric cholesterol biosensor based on reduction graphene oxide-chitosan-ferrocene/platinum nanoparticles modified screen-printed electrode, Journal of Nanoparticle Research 21 (2019) 162-177(SCI, IF=2.127, 3区)



[3] Guiyin Li, Junxiang Zeng, Huiling Liu, Ping Ding, Jintao Liang, Xinmin Nie*, Zhide Zhou*. A fluorometric aptamer nanoprobe for alpha-fetoprotein by exploiting the FRET between 5-carboxyfluorescein and palladium nanoparticles, Microchimica Acta, 186 (2019) 314-322(SCI, IF=5.705，2区)



[4] Zhong Liping, Zou Hang, Huang Yong, Gong Wenlin, He Jian,Tan Jie, Lai Zongqiang, Li Yanmei, Zhou Chaofan, Zhang Guoqing, Li Guiyin*, Yang Nuo*, ZhaoYongxiang*, Magnetic Endoglin Aptamer Nanoprobe for Targeted Diagnosis of Solid Tumor, Journal of Biomedical Nanotechnology 15 (2019) 352-362 (SCI, IF=5.068，2区)



[5] Mingmin Gao, Zhongmin Wang*, Chengxian Yang, Jinliang Ning, Zhide Zhou*, Guiyin Li*, Novel magnetic graphene oxide decorated with persimmon tannins for efficient adsorption of malachite green from aqueous solutions, Colloids and Surface A 566 (2019) 48-57(SCI, IF=3.13，3区)



[6] Zhongmin Wang, Jinliang Ning, Chengxian Yang, Xiaojuan Li, Mingmin Gao,Guiyin Li*, Zhide Zhou*. Functionalization of persimmon tannin with chitosan for the removal of cationic and anionic dyes from aqueous solutions, Desalination and Water Treatment, 150 (2019) 339-347(SCI, IF=1.36，4区)



[7] Xiaojuan Li, Zhongmin Wang, Haijun Liang, Jingliang Ning, Guiyin Li*, Zhide Zhou*. Chitosan modification persimmon tannin bioadsorbent for highly efficient removal of Pb(II) from aqueous environment: the adsorption equilibrium, kinetics and thermodynamics, Environmental Technology, 40 (2019) 112-124 (SCI, IF=1.82，4区)



[8] Yayuan He, Wen Xiao, Guiyin Li, Fei Yang, Pian Wu, Tao Yang, Cuimei Chen, Ping Ding*. A novel lead-ion-imprinted magnetic biosorbent: preparation, optimization and characterization, Environmental Technology, 40 (2019) 499-507(SCI, IF=1.82，4区)



[9] Yong Huang, Jie Tan, Lijie Cui, Zhide Zhou, Sufang Zhou, Zhenghua Zhang, Rong Zheng, Yewei Xue, Mengxin Zhang, Shanshan Li, Nixuan Zhu, Jintao Liang, Guiyin Li*, Liping Zhong, Yongxiang Zhao. Graphene and Au NPs co-mediated enzymatic silver deposition for the ultrasensitive electrochemical detection of cholesterol, Biosensors & Bioelectronics 102 (2018) 560-567 (SCI, IF=9.518，1区)



[10] Yong Huang, Yewei Xue, Junxiang Zeng, Shanshan Li, Zhihong Wang, Chenyang Dong, Guiyin Li*, Jintao Liang*, Zhide Zhou*, Non-enzymatic electrochemical hydrogen peroxide biosensor based on reduction graphene oxide-persimmon tannin?platinum nanocomposite, Materials Science & Engineering C-Materials for Biological Applications, 92 (2018) 590-598 (SCI, IF=5.07，2区).



[11] Xiaojuan Li, Zhongmin Wang*, Jingliang Ning, Mingmin Gao, Wenbin Jiang, Zhide Zhou*,Guiyin Li*.Preparation and characterization of a novel polyethyleneimine cation-modified persimmon tannin bioadsorbent for anionic dye adsorption, Journal of Environmental Management 217 (2018) 305-314 (SCI, IF=4.865，2区).



[12] Guiyin Li, Shanshan Li, Zhihong Wang, Yewei Xue, Chenyang Dong, Junxiang Zeng,Yong Huang∗, Jintao Liang∗, Zhide Zhou∗. Label-free electrochemical aptasensor for detection of alpha-fetoprotein based on AFP-aptamer and thionin/reduced graphene oxide/gold nanoparticles, Analytical biochemistry 547 (2018) 37-44 (SCI, IF=2.34，3区).



[13] Zhide Zhou,Le Zhao ,ZhihongWang,Wen Xue, YunxiaoWang,Yong Huang, Jintao Liang, Jiejing Chen?, Guiyin Li?, Colorimetric detection of 1,5-anhydroglucitol based on graphene quantum dots and enzyme-catalyzed reaction, International Journal of Biological Macromolecules 112 (2018) 1217-1224 (SCI, IF=3.909, 2区).



[14] Pian Wu, Ruixue Huang, Guiyin Li, Yayuan He, Cuimei Chen, Wen Xiao, Ping Ding, Optimization of Synthesis and Modification of ZnSe/ZnS Quantum Dots for Fluorescence Detection of Escherichia coil.Journal of Nanoscience and Nanotechnology18 (2018) 3654-3659 (SCI, IF=1.354, 4区).



[15] Yulin Yuan, Shanshan Li, Yewei Xue, Jintao Liang, Lijie Cui, Qingbo Li , Sufang Zhou, Yong Huang, Guiyin Li*, Yongxiang Zhao. A Fe3O4@Au-based pseudo-homogeneous electrochemical immunosensor for AFP measurement using AFP antibody-GNPs-HRP as detection probe, Analytical biochemistry, 534 (2017) 56-63 (SCI, IF=2.34，3区)



[16] Zhongmin Wang, Xiaojuan Li, Haijun Liang, Jingliang Ning, Zhide Zhou, Guiyin Li*, Equilibrium, kinetics and mechanism of Au3+, Pd2+ and Ag+ ionsadsorption from aqueous solutions by graphene oxide functionalized persimmon tannin. Materials Science & Engineering C-Materials for Biological Applications, 79 (2017) 227-236 (SCI, IF=5.070，2区)



[17] Huang Y, Cui LJ, Xue YW, Zhang SB, Zhu NX, Liang JT*, Li GY*, Ultrasensitive cholesterol biosensor based on enzymatic silver deposition on gold nanoparticles modified screen-printed carbon electrode, Materials Science & Engineering C-Materials for Biological Applications, 2017,77:1-8 (SCI, IF= 5.070，2区)



[18] Liang JT, Zhu NX, Li SS, Jia HQ, Xue YW, Cui LJ, Huang Y*, Li GY*, Light-addressable potentiometric sensor with gold nanoparticles enhancing enzymatic silver deposition for 1,5-anhydroglucitol determination, Biochemical Engineering Journal, 2017,123: 29-37 (SCI, IF=3.371，2区)



[19] Zhide Zhou, Yong Huang, Jintao Liang, YulinYuan, Weizhi Mo, Guiyin Li*. Rapamycin loaded magnetic graphene oxide nanoparticles as tumor-targeted drug delivery system: synthesis and in vitro characterization. Journal of Controlled Release 259 (2017) e37-e38 (SCI, IF=7.877, 1区)



[20] Bai ZH#, Li GY#, Liang JT, Su J, Zhang Y, Chen HZ, Huang Y*, Sui WG*, Zhao YX*. Non-enzymatic Electrochemical Biosensor Based on Pt NPs/RGO-CS-Fc Nano-hybrids for the Detection of Hydrogen Peroxide in Living Cells. Biosensors & Bioelectronics, 2016, 82:185-194 (SCI, IF=9.518,1区)



[21] Zhou ZD, Huang Y, Liang JT, Ou ML, Chen JJ, Li GY*, Extraction, purification and anti-radiation activity of persimmon tannin from Diospyros kaki L.f, Journal of Environmental Radioactivity, 2016,162-163:182-188 (SCI, IF=2.263，3区).



[22] Liang JT, Guan MY, Huang GY, Qiu HM, Chen ZC, Li GY*, Huang Y*. Highly sensitive covalently functionalized light-addressable potentiometric sensor for determination of biomarker. Materials Science & Engineering C-Materials for Biological Applications, 2016, 63:185-191(SCI, IF= 5.070, 2区)



[23] Cuimei Chen, Ming Zhong, Guiyin Li, Fei Yang, Ruixue Huang, Wen Xiao, Ping Ding. Optimization on preparation conditions of calcium-crosslinked modified chitosan as potential matrix material for theophylline sustained-release beads and its evaluation of release kinetics. Journal of Alloys and Compounds 658 (2016) 348-355(SCI, IF= 3.779, 2区)



[24] Li GY, Cao LL, Zhou ZD, Chen ZC, Huang Y*, Zhao YX*. Rapamycin Loaded Magnetic Fe3O4/Carboxymethylchitosan Nanoparticles as Tumor-targeted Drug Delivery System: Synthesis and In Vitro Characterization. Colloids and Surfaces B-Biointerfaces, 2015,128:379-388 (SCI, IF=3.973, 2区)



[25] Zhou ZD, Liu FL, Huang Y, Wang ZM, Li GY*. Biosorption of palladium(II) from aqueous solution by grafting chitosan on persimmon tannin extract. International Journal of Biological Macromolecules, 2015,77:336-343(SCI, IF=3.909, 2区)



[26] Huang Y, Zhou ZD, Liang JT, Chen ZC, Ding P, Cao LL, Li GY*. Preparation, characterisation and in vitro cytotoxicity studies of chelerythrine-loaded magnetic Fe3O4@O-carboxymethylchitosan nanoparticles. Journal of Experimental Nanoscience, 2015, 10: 483-498 (SCI, IF=1.362,4区)



[27] Huang Y#, Yuan YL#, Zhou ZD#, Liang JT, Cheng ZC, Li GY*. Optimization and evaluation of chelerythrine nanoparticles composed of magnetic multiwalled carbon nanotubes by response surface methodology. Applied Surface Science, 2014, 292,378-386 (SCI,IF=4.439,2区)



[28] Cao LL, Li GY*, Yang DL, Liu JJ*, Huang Y. Application of response surface methodology to formulation optimization of rapamycin loaded magnetic Fe3O4/carboxymethylchitosan nanoparticles. Journal of Macromolecular Science Part A-Pure and Applied Chemistry, 2013, 50(8) 894-904 (SCI, IF=1.163, 4区)



[29] Liu FL, Wang ZM，Li GY*, Adsorption of Ag+ by persimmon tannins immobilized on collagen fiber. Desalination and Water Treatment, 2013, 8:1-8 (SCI，IF=1.36, 4区)



[30] Huang Y*, Zhu J, Li GY, Chen Z, Jiang JH*, Shen GL, Yu RQ. Electrochemical detection of point mutation based on surface hybridization assay conjugated allele-specific polymerase chain reaction. Biosensors and Bioelectronics. 2013, 42: 526-531 (SCI, IF=9.518,1区)



[31] Li GY, Zhong M, Zhong YD, Ding P, Huang Y*. Formulation optimization of chelerythrine loaded O-carboxymethylchitosan microspheres using response surface methodology. International Journal of Biological Macromolecules, 2011, 49(5): 970-978 (SCI)



[32] Li GY, Zhong M, Zhou ZD, Ding P, Li YJ*. Novel carboxymethyl chitosan microspheres for controlled delivery of chelerythrine. Journal of Macromolecular Science Part A-Pure and Applied Chemistry, 2011, 48(10): 904-911 (SCI)



[33] Li GY, Zhou ZD, Li YJ*, Huang KL, Zhong M. Surface functionalization of chitosan-coated magnetic nanoparticles for covalent immobilization of yeast alcohol dehydrogenase from saccharomyces cerevisiae. Journal of Magnetism and Magnetic Materials, 2010, 322(24): 3862-3868 (SCI)



[34] Zhou ZD, Li GY*, Li YJ*. Immobilization of saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate-chitosan beads. International Journal of Biological Macromolecules, 2010, 47(1): 21-26 (SCI)



[35] Zeng JX, Tang ZJ, Li GY*. TMB colorimetric cholesterol biosensor based on reduced graphene oxide-Hemin nanocomposites. IOP Conference Series: Earth and Environmental Science 252(2019), 022134(EI)



[36] Lu CT, Wei DC, Li GY*. A fluorescence turn-on biosensor based on gold nanoclusters and aptamer for alpha fetoprotein detection. IOP Conference Series: Earth and Environmental Science 218(2019), 012106(EI)



[37] Xue YW, Huang Y, Zhou ZD, Li GY*, Non-enzymatic glucose biosensor based on reduction graphene oxide-persimmon tannin-Pt-Pd nanocomposite, IOP Conference Series: Material Science and Engineering, 382 (2018) 022016 (EI)



[38] Zhou ZD, Wang ZH, Zhao L, Li GY*, Preparation and properties of magnetic-fluorescent endoglin aptamer nanoprobe, IOP Conference Series: Material Science and Engineering, 382 (2018) 022035(EI)



[39] Li SS, Liang JT, Zhou ZD, Li GY*, An electrochemical immunosensor for AFP measurement based on the magnetic Fe3O4@Au@CS nanomaterials, IOP Conference Series: Material Science and Engineering, 382 (2018) 022017 (EI)



[40] Zhide Zhou, Guizhi Huang, Li GY*, Microwave drying of Siraitia grosvenorii and its effect on the Vitamin C content, 2018 7th International Conference on Energy, Environment and Sustainable Development, (2018) 1725-1728(EI)



[41] Xiaojuan Li, Zhide Zhou, Yong Huang, Li Guiyin*, Chitosan/polyvinylpyrrolidone hydrogels for cimetidine release, Material Science and Engineering - Proceedings of the 3rd Annual International Conference on Material Science and Engineering, ICMSE 2015 (2016) 191-195(EI).



[42] Huang GY, Ma LF, Guan MY, Liang JT, Huang Y*, Li GY*. A novel hIg E Aptamer Biosensor Base on core-shell Fe3O4@Au Magnetic Composite nanoparticles. Advanced Materials Research, 2015,1118:170-175 (EI)



[43] Ma LF, Huang GY, Guan MY, Huang Y, Shi GW, Liang JT, Li GY*，A silicon resonant micro-cantilever biosensor with closed-loop self-excitation system for biomacromolecular detection. Advanced Materials Research, 2014, (1030-1032), 2320-2325 (EI)



[44] Huang Y, Zhou ZD, Liang JT, Li GY*. Preparation and Characterization of Chelerythrine Nanoparticles Composed of Magnetic Multiwalled Carbon Nanotubes. Advanced Materials Research, 2013, 788: 127-131 (EI).







学术著作

科研项目

1) 基于石墨烯的GPC3适配体纳米生物传感器靶向诊断肝癌的研究(**)，国家自然科学基金，34万，2018.01-2021.12，主持，进行。



2) 近红外荧光/磁性Endoglin适配体靶向肺癌双模态成像研究（**），国家自然科学基金，48万，2015.01-2018.12，主持，结题。



3) 柿单宁/石墨烯/Pt-Pd无酶血糖传感器研究（2016GXNSFAA380080），广西自然科学基金，5万，2016.09 - 2019.08，主持，结题。



4) 特异性适配体生物传感器靶向诊断肝癌的新技术研究 (**)，，国家自然科学基金重点项目，320万元，2015/01-2019/12，主要参与（第三），结题。



5) 基于Endoglin靶的光/磁探针用于肝癌多模态成像研究（2013M531916），5.0万，中国博士后科学基金，2014.1-2016.12，主持，结题。



6) 柑桔果皮黄酮类及辛弗林生物活性物质高校提取关键技术研究（桂科转），10万，广西科技厅科学计划与研究项目，2014.01-2016.04，主持，结题。



7) 一种治疗骨质增生、椎间盘突出的外敷药的稳定化研究（桂科攻**），16万，广西科技厅科学计划与研究项目，2012.01-2016.04，主持，结题。



8) 罗汉果有效成份综合提取与产品开发， 2014年第一批自治区企业技术改造资金（工业创新发展）项目，50万，2014.04-2016.07，主持，结题。



专利及知识产权

[1] 李桂银;李珊珊; 薛叶薇；黄勇；梁晋涛; 王志宏;董辰杨；周治德.一种基于石墨烯、硫瑾和核酸适配体检测甲胎蛋白的方法. 申请日：2017.09.07, 中国，发明专利, 授权公告号：CNB, 授权公告日：2019.10.18, 专利号：ZL 9.1，证书号，第**号



[2] 李桂银;李珊珊; 周治德; 梁晋涛; 陆春婷. 一种复合纳米材料电化学检测甲胎蛋白的方法.申请日：2018.02.08, 中国，发明专利, 授权公告号：CNB, 授权公告日：2019.07.30, 专利号：ZL 2.9，证书号，第**号



[3] 李桂银; 李珊珊; 王志宏; 杨凤仙;周治德; 黄勇; 梁晋涛;李昊远. 一种柿单宁@石墨烯@Pt-Pd无酶传感器检测血糖的方法. 申请日：2016.09.23, 中国，发明专利, 授权公告号：CNB. 授权公告日：2018.12.21. 专利号：ZL 3.3，证书号，第**号



[4] 李桂银, 谢冬养, 周治德,卢照凯. 一种从柑橘小果、皮中提取橙皮苷和新橙皮苷的方法, 申请日：2015.12.08, 中国，国家发明专利，授权公告号：CNB. 授权公告日：2018.08.17. 专利号：ZL7.0，证书号，第**号



[5] 李桂银, 周治德, 谢冬养, 赖廷晓，应启实,卢照凯，杨涛，刘雯，叶航. 一种从柑橘皮、果中提取橙皮苷、新橙皮苷和辛弗林的方法, 申请日：2015.12.08中国，国家发明专利，授权公告号：CNB. 授权公告日：2018.11.13. 专利号：ZL9.6，证书号，第**号



[6] 李桂银, 周治德, 谢冬养, 应启实,卢照凯，叶世伟，赖廷晓，杨涛，黄荣爱. 一种提取橙皮苷、新橙皮苷和辛弗林的方法, 申请日：2015.12.08,中国，国家发明专利，授权公告号：CNB. 授权公告日：2018.11.23. 专利号：ZL2.4，证书号，第**号



[7] 李桂银, 谢冬养, 周治德,李昊远，叶世伟，应启实,杨裴庆，卢照凯，钟祥荣. 从柑橘皮、果中提取橙皮苷和新橙皮苷的方法, 申请日：2015.12.08,中国，国家发明专利，授权公告号：CNB. 授权公告日：2018.11.02, 专利号：ZL7.3，证书号，第**号



[8] 李桂银，周治德，吴媛燕，陈真诚，莫伟志.一种具有抗电离辐射作用的复方柿单宁芦荟胶，申请日：2014.12.23, 授权公告日：2017.06.06，授权公告号：CNB, 中国，国家发明专利，专利号：ZL 2. 9，证书号，第**号



[9] 李桂银, 周治德, 黄勇, 杨裴庆, 刘雯, 秦亚珍. 一种罗汉果复方胶囊的制备方法, 申请日：2015.06.03, 授权公告日：2017.01.18, 中国，国家发明专利，专利号：ZL7.5，证书号，第**号



[10] 李桂银，周治德，黄勇，梁晋涛，马龙飞，管明源，黄国银. 一种用碳纳米管微悬臂梁生物传感器检测血小板衍生化生长因子浓度为0.5-10毫克/毫升的方法，申请日：2014.06.18, 授权公告日：2016.05.11, 中国，国家发明专利，专利号：ZL4.2. 证书号，第**号



[11] 李桂银，周治德, 黄勇, 操良丽, 陈真诚. 一种雷帕霉素/磁性羧甲基壳聚糖纳米载药微球的制备方法，申请日：2012.12.20, 授权公告日：2014.05.21，授权公告号：CNB，中国，国家发明专利，专利号：ZL2.2，证书号，第**号



[12] 李桂银, 周治德, 王仲民, 操良丽, 崔健, 周怀营. 一种小分子含量多的涩柿子粉的制备方法，申请日：2012.2.24，授权公告日：2013.4.10，授权公告号：CNB，中国，国家发明专利，专利号：ZL9.X, 证书号，第**号



[13] 李桂银, 周治德,刘绍.一种冷冻干燥用的芦荟浓缩汁配制方法，申请日：2008.04.18，中国，国家发明专利，授权公告号：CNB， 授权公告日：2012.03.21，专利号：ZL4.3



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