何凯 教授、博士生导师博士 美国俄克拉荷马大学植物与微生物系 2008 学士 四川大学生命科学院 2000 硕士 四川大学生命科学院 2003
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所在部门: 植物学与植物生理学研究所
办公室: 逸夫生物楼614
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电子邮件: hekai@
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学习经历2008 美国俄克拉荷马大学植物与微生物系 博士
2003 四川大学生命科学院 硕士
2000 四川大学生命科学院 学士
工作经历2013至今 兰州大学生命科学学院 教授
2009-2013美国加州大学伯克利分校植物与微生物系 博士后
2005-2008美国俄克拉荷马大学 教学助理
2003-2008 美国俄克拉荷马大学 研究助理
社会工作
研究方向本课题组主要关注植物逆境适应过程中的分子机制:
(1)植物氮胁迫与氮信号(Nitrogen Stress and Signaling in Plants):氮素(Nitrogen, N)作为植物营养的三大要素之一,是构成植物体多种有机化合物的基本成分。植物主要以无机态氮:铵态氮(NH4+)、硝态氮(NO3-)的形式吸收氮素,而氮素会直接影响植物的各项生命活动。本团队致力于研究植物对氮素营养的感知、吸收、转运以及氮素信号应答机制,首次发现硝酸根离子外排通道SLAH3通过平衡植物硝态氮的吸收,在缓解植物铵盐毒害过程中发挥重要作用。主要研究方向包括三方面:A.植物对高铵氮源逆境适应的分子机理;B.植物对氮素的感知与信号转导机制;C.植物对氮素的塑性反应分子机理。
(2)拟南芥类受体蛋白的生物学功能(Functional Analyses of Receptor-Like Proteins, RLPs):细胞表面的受体激酶(Receptor-Like Kinases, RLKs)在细胞与周围环境之间的信号转导过程中起至关重要的作用。与类受体蛋白激酶不同,类受体蛋白只包含胞外识别域、跨膜域,通常缺乏胞内激酶结构域。本团队正在利用各种遗传学、分子生物学以及组学手段对RLP中尚未揭示功能的组分开展生物学功能研究。
项目成果主持科研项目:
国家自然科学基金面上项目“离子通道蛋白 SLAH3 调控植物对高铵和低 pH 胁迫响应的分子机理研究”、(No.**)2019-2022,60万,主持,在研;
国家自然科学基金面上项目“拟南芥受体激酶 BAK1 和 BKK1 介导细胞死亡分子机理”(No.**)2015-2018,80万,主持,结题;
中央高校基本科研业务费专项资金-自由探索-面上项目“BED1依赖 MAPK 途径调节植物花序发育”(lzujbky-2016-75)2016-2017,10万,主持,结题;
中央高校基本科研业务费专项资金-科研创新团队培育项目“植物免疫与逆境响应的分子机制”(lzujbky-2015-ct01)2015-2016,50万,主持,结题;
中央高校基本科研业务费专项资金-自由探索-重点项目“拟南芥蛋白激酶 SnRK1 的生物学功能研究”(lzujbky-2015-k17)2015-2016,14万,主持,结题;
中央高校基本科研业务费专项资金-自由探索-面上项目“拟南芥阴离子通道蛋白介导氮平衡的分子机理”(lzujbky-2014-84)2014-2015,10万,主持,结题。
参与科研项目:
国家作物转基因重大专项子课题,2016ZX08009-003-002,2016-2020,200万,参与,结题;
国家自然科学基金植物激素作用的分子机理重大研究集成项目“ BAK1 调控油菜素内酯(BR)信号转导及器官发育的分子机制”(**)2014-2015,200万,参与,结题。
荣誉、获奖
教学及指导研究生情况本科生《细胞信号转导》教学
研究生《Cell signal transduction》全英文教学
目前指导博士后1人,博士研究生6人,硕士研究生6人。
欢迎欢迎有志于植物逆境胁迫应答与信号转导研究的同学加盟!
发表论文及专著(*corresponding author; §contributed equally)
1. Fang, X., Zhang, Y., Cheng, B., Luan, S.*, & He, K.* (2021). Evidence for the involvement of AtPiezo in mechanical responses. Plant Signaling & Behavior, DOI:10.1080/**.2021.**
2. Sun, D., Fang, X., Xiao, C., Ma, Z., Huang, X., Su, J., Li, J., Wang, J., Wang, S., Luan, S., He, K.* (2021) Kinase SnRK1.1 regulates nitrate channel SLAH3 engaged in nitrate-dependent alleviation of ammonium toxicity. Plant Physiology,DOI:10.1093/plphys/kiab057
Commented in: Wang, P. (2021). Friend or foe: how nitrate antagonizes ammonium toxicity. Plant Physiology, kiab095. Advance online publication. https://doi.org/10.1093/plphys/kiab095
3. Fang, X., Liu, B., Shao, Q., Huang, X., Li, J., Luan, S.*, and He, K.* (2021) AtPiezo plays an important role in root cap mechanotrasdiction. International Journal of Molecular Sciences 22, 467.
4. Chen, L., Zhao, M., Wu, Z., Chen, S., Rojo, E., Luo, J., Li, P., Zhao, L., Chen, Y., Deng, J., Cheng, B., He, K., Gou, X., Li, J., Hou, S.* (2020) RNA polymerase II associated proteins regulate stomatal development through directly interacting with the stomatal transcription factors in Arabidopsis thaliana. New Phytologist,DOI:10.1111/nph.17004
5. Wu, Y., Gao, Y., Zhan, Y., Kui, H., Liu, H., Yan, L., Kemmerling, B., Zhou, J-M., He, K.*, Li, J.* (2020) Loss of the common immune coreceptor BAK1 leads to NLR-dependent cell death. Proc. Natl. Acad. Sci. USA 117(43), 27044-27053.
6. Lu, X., Shi, H., Ou, Y., Cui, Y., Chang, J., Peng, L., Gou, X., He, K., Li, J.* (2020) RGF1-RGI1, a peptide-receptor complex, regulates Arabidopsis root meristem development via a MPAK signaling cascade. Molecular Plant 13, 1594-1607.
7. Xun, Q., Wu, Y., Li, H., Chang, J., Ou, Y., He, K., Gou, X., Tax, F. E., Li, J.* (2020) Two receptor-like protein kinases, MUSTACHES and MUSTACHES-LIKE, regulate lateral root development in Arabidopsis thaliana, New Phytologist 227, 1157-1173.
8. Chang, J., Li, X., Fu, W., Wang, J., Yong, Y., Shi, H., Ding, Z., Kui, H., Gou, X., He, K., Li, J.* (2019) Asymmetric distribution of cytokinins determines root hydrotropism in Arabidopsis thaliana. Cell Research 29(12), 984-993.
9. Li, H., Cai, Z., Wang, X., Li, M., Cui, Y., Cui, N., Yang, F., Zhu, M., Zhao, J., Du, W., He, K., Yi, J., Tax, F.E., Hou, S., Li, J., Gou, X.* (2019) SERK receptor-like kinases control division patterns of vascular precursors and ground tissue stem cells during embryo development in Arabidopsis. Molecular Plant 12(7), 984-1002.
10. Lv, M., Li, M., Chen, W., Wang, Y., Sun, C., Yin, H., He, K., and Li, J.* (2018) Thermal-enhanced bri1-301 instability reveals a plasma membrane protein quality control system in plants. Frontiers in Plant Science 9, 1620.
11. Cui, Y., Hu, C., Zhu, Y., Cheng, K., Li, X., Wei, Z., Xue, L., Lin, F., Shi, H., Yi, J., Hou, S., He, K., Li, J., Gou, X.* (2018) CIK Receptor kinases determine cell fate specification during early anther development in Arabidopsis. Plant Cell 30, 2383-2401.
12. Hu, C., Zhu, Y., Cui, Y., Cheng, K., Liang, W., Wei, Z., Zhu, M., Yin, H., Zeng, Li, Xiao, Y., Lv, M., Yi, J., Hou, S., He, K., Li, J., Gou, X.* (2018). A group of receptor kinases are essential for CLAVATA signaling to maintain stem cell homeostasis. Nature Plants 4, 205-211.
13. Wei, Z., Yuan,T., Tarkowská,D., Kim,J., Nam,H.G.,Novák,O., He, K., Gou, X. and Li, J.* (2017) Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4 and PIF5. Plant Physiology 174, 1260-1273.
14. Gao Y., Wu Y., Du J., Zhan Y., Sun D., Zhao J., Zhang S., Li J. andHe K.* (2017) Both light-induced SA accumulation and ETI mediators contribute to the cell death regulated by BAK1 and BKK1.Frontiers in Plant Science25;8:622.
15. Wei, Z., Yuan, T., Tarkowská, D., Kim, J., Nam, H.G., Novák, O.,He, K., Gou, X. and Li, J. (2017) Brassinosteroid biosynthesis is modulated via a transcription factor cascade of COG1, PIF4 and PIF5.Plant Physiology174(2):1260-1273
16. He, K.*, and Wu, Y. (2016) Receptor-Like Kinases and Regulation of Plant Innate Immunity.Enzymes, 40:105-142
17. Ou, Y., Lu, X., Zi, Q., Xun, Q., Zhang J., Wu, Y., Shi, H., Wei, Z., Zhao, B., Zhang, X.,He, K., Gou, X., Li, C., Li, J. (2016) RGF1 INSENSITIVE 1 to 5, a group of LRR receptor-like kinases, are essential for the perception of root meristem growth factor 1 inArabidopsis thaliana.Cell Research, 26(6), 686-698
18. Chen, L., Guan, L., Qian, P., Xu, F., Wu, Z., Wu, Y.,He, K., Gou, X., Li, J., and Hou, S. (2016) NRPB3, the third largest subunit of RNA polymerase II, is essential for stomatal patterning and differentiation in Arabidopsis.Development, 2016 Mar 17. pii: dev.129098.
19. Du, J., Gao, Y., Zan, Y., Zhang, S., Wu, Y., Xiao, Y., Zou, B.,He, K., Gou, X., Li, G., Lin, H., Li, J. (2016) Nucleocytoplasmic trafficking is essential for BAK1 and BKK1-mediated cell-death control.Plant Journal85(4), 520-531.
20. Wu, Y., Xun, Q., Guo, Y., Zhang, J., Cheng, K., Shi, T.,He, K., Hou, S., Gou, X., Li, J. (2016) Genome-wide expression pattern analyses of the Arabidopsis leucine-rich repeat receptor-like kinases.Molecular Plant9(2), 289-300.
21. Wu, W., Wu Y., Gao, Y., Li, M., Yin, H., Lv, M., Zhao, J., Li, J.,He, K.* (2015) Somatic embryogenesis receptor-like kinase 5 in the ecotype Landsberg erecta of Arabidopsis is a functional RD LRR-RLK in regulating brassinosteroid signaling and cell death control.Frontiers in Plant Science6:852. doi: 10.3389/fpls.2015.00852
22. Zheng X.§,He K.§, Kleist T., Chen F., Luan S. (2015) Anion channel SLAH3 functions in nitrate-dependent alleviation of ammonium toxicity in Arabidopsis.Plant, Cell and EnvironmentMar;38(3):474-86
23. Hou C., Tian W., Kleist T.,He K., Garcia V., Bai F., Hao Y., Luan S., Li L. (2014) DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes.Cell Research2014 Feb 7. doi: 10.1038/cr.2014.14.
24. He K., Xu S., Li J. (2013) BAK1 directly regulates brassinosteroid perception and BRI1 activation.Journal of Integrative Plant Biology55(12), 1264–1270. ( Cover article)
25. Lee SC., Lim CW., Lan W.,He K., Luan S. (2013) ABA signaling in guard cells entails a dynamic protein-protein interaction relay from the PYL-RCAR family receptors to ion channels.Molecular Plant6:528-38.
26. Gou, X.§, Yin, H.§,He, K.§, Du, J., Yi., J., Xu, S. Lin, H., Clouse, SD., Li, J. (2012) Genetic Evidence for an Indispensable Role of Somatic Embryogenesis Receptor Kinases in Brassinosteroid Signaling.PLoS Genetics8(1): e**. doi:10.1371/journal.pgen.**.
27. Li J., Du J.,He K., Gou X. (2011). Control of cell death by Receptor Kinases inArabidopsis thaliana. In "Receptor-like Kinases in Plants: From Development to Defense (eds Birgit Kemmerling and Frans Tax)". Signaling and Communication in Plants (ed. Frantisek Baluska, Springer Publisher),Volume 13, pp79-91.
28. Yang, H., Gou, X.,He, K., Xi, D., Du, J., Lin, H., Li, J. (2010) Arabidopsis BAK1 and BKK1 confer reduced susceptibility to Turnip Crinkle Virus.European Journal of Plant Pathology127: 147-156.
29. Gou, X.,He, K., Yang, H., Yuan, T., Lin, H., Clouse, S.D., Li, J. (2010) Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana.BMC Genomics11(1):19
30. He, K., Gou, X., Powell, R.A., Yang, H., Yuan, T, Guo, Z., and Li, J.(2008) Receptor-like protein kinases, BAK1 and BKK1, regulate a light-dependent cell-death control pathway.Plant Signaling & Behavior3 (10), 813-815.
31. Wang, X., Kota, U.,He, K., Blackburn, K., Li, J., Goshe, M.B., Huber, S.C., and Clouse, S.D.(2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase pair impacts early events in brassinosteroid signaling.Developmental Cell15, 220-235.
32. He, K., Gou, X., Yuan, T., Lin, H., Asami, T., Yoshida, S., Russell, S.D., Li, J. (2007) BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell death pathways.Current Biology17, 1109-1115.
33. Heese, A., Hann, D.R., Gimenez-ibanex, S., Jones, A.,He, K., Li, J., Schroeder, J.I., Peck, S.C., Rathjen, J.P. (2007) The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants.Proceedings of the National Academy of Sciences of the United States of America104, 12217-12222.
34. Yuan, T., Fujioka, S., Takatsuto, S., Matsumoto, S., Gou, X.,He, K., Russell, S.D., and Li, J.(2007) BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana.The Plant Journal51 (2), 220–233.
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