顾 颖 副教授/硕导

学科（方向）：抗病毒的单抗筛选、基于表位的抗原研究等
办公电话：+86-
邮 箱：guying@xmu.edu.cn



研究领域
抗病毒的单抗筛选：利用细胞模型和重组抗原等进行抗病毒的单抗筛选，如HIV中和单抗、识别免疫优势表位单抗等的筛选。
功能性表位研究：应用生物传感器、分析超离、微量热等方法研究抗原-抗体的相互作用，尝试解析免疫复合物的空间结构，鉴定功能性表位的特征。
基于表位的抗原研究：利用基因工程重组技术表达获得含有功能表位的抗原或颗粒化抗原。
学习和工作经历
1996年，厦门大学生物学专业，学士学位；
2006年，厦门大学生化与分子生物学专业/细胞生物学专业，博士学位；
2006年，厦门大学生命科学学院助理教授、硕士生导师；
2013年，厦门大学生命科学学院副教授；
2018-2019年，美国国立卫生研究院过敏与传染病研究所疫苗研究中心（NIH-NIAID-VRC），访问****
代表性成果
1、Zheng Q#, Jiang J#, He M#, Zheng Z#, Yu H, Li T, Xue W, Tang Z, Ying D, Li Z, Song S, Liu X, Wang K, Zhang Z, Wang D, Wang Y, Yan X, Zhao Q, Zhang J,Gu Y*,Li S*, Xia N*. Viral neutralization by antibody-imposed physical disruption.Proc Natl Acad Sci U S A. 2019 Dec 9.
2、Liu X#, Chen J#, Wang Z#, Wang D, He M, Qian C, Song S, Chi X, Kong Z, Zheng Q, Wang Y, Yu H, Zhao Q, Zhang J, Li S*,Gu Y*,Xia N. Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection.Emerg Microbes Infect. 2019;8(1):1721-1733.
3、Kong R#, Duan H#, Sheng Z#, Xu K, Acharya P#, Chen X#, Cheng C#, Dingens AS#, Gorman J#, Sastry M#, Shen CH#, Zhang B#, Zhou T#, Chuang GY, Chao CW, Gu Y, Jafari AJ, Louder MK, O'Dell S, Rowshan AP, Viox EG, Wang Y, Choi CW, Corcoran MM, Corrigan AR, Dandey VP, Eng ET, Geng H, Foulds KE,Guo Y, Kwon YD, Lin B, Liu K, Mason RD, Nason MC, Ohr TY, Ou L, Rawi R, Sarfo EK, Sch?n A, Todd JP, Wang S, Wei H, Wu W; NISC Comparative Sequencing Program, Mullikin JC, Bailer RT, Doria-Rose NA, Karlsson Hedestam GB, Scorpio DG, Overbaugh J, Bloom JD, Carragher B, Potter CS, Shapiro L, Kwong PD*, Mascola JR*. Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization.Cell. 2019 Jul 25;178(3):567-584.
4、Zhang Z#, Wei X#, Lin Y, Huang F, Shao J, Qi J, Deng T, Li Z, Gao S, Li S, Yu H, Zhao Q, Li S,Gu Y*,Xia N. HIV-1 Membrane-Proximal External Region Fused to Diphtheria Toxin Domain-A Elicits 4E10-Like Antibodies in Mice.Immunol Lett. 2019 Jul 26. pii: S0165-2478(19)30005-7.
5、Li T#, Zhang Z#, Zhang Z#, Qiao J, Rong R, Zhang Y, Yao Q, Li Z, Shen H, Huang F, Xue W, Gao S, Li S, Zheng Q, Yu H, Zhang J,Gu Y*,Li S*, Xia N. Characterization of native-like HIV-1 gp140 glycoprotein expressed in insect cells.Vaccine. 2019 Mar 7;37(11):1418-1427.
6、Li Z#, Song S#, He M#, Wang D, Shi J, Liu X, Li Y, Chi X, Wei S, Yang Y, Wang Z, Li J, Qian H, Yu H, Zheng Q, Yan X, Zhao Q, Zhang J,Gu Y*,Li S*, Xia N*. Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity.Nat Commun. 2018 Dec 18;9(1):5360.
7、Zhang Z, He M, Bai S, Zhang F, Jiang J, Zheng Q, Gao S, Yan X, Li S*,Gu Y*,Xia N. T = 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display.Viruses. 2018 Nov 26;10(12). pii: E667.
8、Li Z#, Wang D#,Gu Y#,Song S, He M, Shi J, Liu X, Wei S, Li J, Yu H, Zheng Q, Yan X, Baker TS, Zhang J, McLellan JS*, Li S*, Xia N*. Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus.mBio.2017 Sep 26;8(5). pii: e00787-17.
9、Gu Y#,Wei M#, Wang D, Li Z, Xie M, Pan H, Wu T, Zhang J, Li S*, Xia N*. Characterization of an Escherichia coli-derived human papillomavirus type 16 and 18 bivalent vaccine.Vaccine.2017 Aug 16;35):4637-4645.
10、Zhang Z, Zhang F, Bai S, Qiao J, Shen H, Huang F, Gao S, Li S,Gu Y*,Xia N. Characterization and epitope mapping of a panel of monoclonal antibodies against HIV-1 matrix protein.Biotechnol Appl Biochem.2018 Nov;65(6):807-815.
11、Zhang Z, Wang L, Bai S, Qiao J, Shen H, Huang F, Gao S, Li S, Li S,Gu Y*,Xia N. Expression, Purification and Characterization of Hiv-1 Capsid Precursor Protein p41.Protein J.2018 Apr;37(2):194-202.
12、Zhang Z, Li S,Gu Y*,Xia N. Antiviral Therapy by HIV-1 Broadly Neutralizing and Inhibitory Antibodies.Int J Mol Sci. 2016 Nov 18;17(11). pii: E1901.
13、Zhang Z, Zhang F, Yang C, Qi J, Gao S, Li S,Gu Y*,Xia N. HIV-1 Capsid as a Target for Antiviral Therapy,Journal of AIDS & Clinical Research, 2016.1.15, 7(1)
14、Gu Y#,Tang X#, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S*, Xia N*, Sivaraman J*.Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody.Cell Res. 2015 May;25(5): 604-20.
15、Hou W, Fang C, Liu J, Yu H, Qi J, Zhang X, Yuan R, Xiong D, Gao S, Adam Yuan Y, Li S,Gu Y*, Xia N. Molecular insights into the inhibition of HIV-1 infection using a CD4 domain-1-specific monoclonal antibody.Antiviral Res.2015 Aug 8. pii: S0166-3542(15)00190-4.
16、Gu Y, Cao F, Wang L, Hou W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly.PLoS One. 2013 Apr 19;8(4):e61314.
17、Gu Y, Hou W, Xu C, Li S, Shih JW, Xia N*. The enhancement of RNAi against HIV in vitro and in vivo using H-2K(k) protein as a sorting method.J Virol Methods. 2012 Jun;182(1-2):9-17.
18、Shen L#,Gu Y#,Sun L, Yang Y, Wang F, Li Y, Bi S*. Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China.J Med Virol. 2012 Mar;84(3):445-9.
19、Xu CY,Gu Y*,Hou WH, Que YQ, Gao SG, Cheng T, Xia NS*. Tetracysteine as a Reporter for Gene Therapy.Biomed Environ Sci. 2009 Dec;22(6):496-501.