李光LI Guang, Ph.D.

副教授
E-mail:guangli@xmu.edu.cn

1999-2003，安徽师范大学，本科
2003-2006，厦门大学，硕士
2006-2010，厦门大学，博士
2008-2010，牛津大学，联合培养博士
1999-2003, Anhui Normal University，B.S. degree
2003-2006, Xiamen University，M.S. degree
2006-2010, Xiamen University, Ph.D. degree
2008-2010, Oxford University, Joint training doctoral students

研究领域
文昌鱼是现存生物中与脊椎动物最相似的无脊椎动物，因此是研究脊椎动物起源及其胚胎发育机制的理想模型。我们课题组主要运用基因敲除、转基因、显微注射、原位杂交、扫描电镜等试验手段，研究文昌鱼早期胚胎发育机制，特别是体轴建立机制，以期对脊椎动物器官起源与发育机制有更深入的理解。
Research Area
Among the living invertebrates, amphioxus shows most similarities with vertebrates in respect to either body structure, developmental process, or genome content, which makes amphioxus an ideal model organism for studying developmental and evolutionary mechanisms of vertebrate embryogenesis. We are using methods including gene knockout, transgene, microinjection, whole-mount in situhybridization and scanning electron microscopeto dissect the molecular mechanisms of amphioxus embryogenesis, aiming to illustrate how the morphological and developmental complexity of vertebrates has evolved.





代表性论文(Selected Publications)
1. Ren QQ^#, Zhong YH^#, Huang X, Leung B, Xing CF, Hu GW, Wang YQ*, Shimeld SM*, Li G*. 2020. Step-wise evolution of neural patterning by Hedgehog signalling in chordates. Nature Ecology & Evolution, Sep;4(9):1247-1255.
2. Zhong YH^#, Herrera-úbeda C^#, Garcia-Fernàndez J, Li G*, Holland PW*, 2020. Mutation of amphioxus Pdx and Cdx demonstrates conserved roles for ParaHox genes in gut, anus and tail patterning. BMC Biology, 16;18(1):68.
3. Zhu X^#, Shi CG, Zhong YH, Liu X, Yan QN, Wu XT, Wang YQ*, Li G*, 2020. Cilia-driven asymmetric Hedgehog signalling determines the amphioxus left-right axis by controlling Dand5 expression. Development, 147(1). pii: dev182469. doi: 10.1242/dev.182469.
4. Shi CG^#, Wu XT, Su LR, Shang CQ, Li XW, Wang YQ*, Li G*, 2020. A ZZ/ZW sex chromosome system in cephalochordate amphioxus. Genetics, 214(3):617-622
5. Su LR^#, Shi CG, Huang X, Wang YQ, Li G*, 2020. Application of CRISPR/Cas9 nuclease in amphioxus genome editing. Genes (Basel), 2020 Nov 5;11(11):1311.
6. Yuan L^#, Wang YQ, Li G*. 2020. Differential expression pattern of two Brachyury genes in amphioxus embryos. Gene Expr Patterns, Dec;38:119152. doi: 10.1016/j.gep.2020.119152.
7. Zhang HY, Chen S, Shang CQ, Wu XT, Wang YQ*, Li G*, 2019. Interplay between Lefty and Nodal signaling is essential for the organizer and axial formation in amphioxus embryos. Developmental biology, 456(1):63-73.
8. Huang JF^#, You WW^#, Xu ZW, Yan QN, Shi CG, Tang B, Luo X, Li, G.*, Ke, CH*, 2019. An Effective Microinjection Method and TALEN-Mediated Genome Editing in Pacific Abalone. Marine biotechnology, 21(4):441-447.
9. Hu GW, Li G, Wang H, Wang YQ* (2017), Hedgehog participates in the establishment of left-right asymmetry during amphioxus development by controlling Cerberus expression. Development, 144(24):4694-4703. Co-first author
10. Li G, Liu X, Xing CF, Zhang HY, Shimeld SM, Wang YQ. (2017) Cerberus-Nodal-Lefty-Pitx signaling cascade controls left-right asymmetry in amphioxus. PNAS, 14(114): 3684–3689
11. Li G, Wang J, Yuan L, Wang H, Wang YQ. (2015) A simple method for selecting spawning-ready individuals out from laboratorial cultured amphioxus population. Journal of experimental zoology, 324(7):629-635.
12. Feng J, Li G, Liu X, Wang J, Wang YQ. (2014) Functional analysis of the promoter region of amphioxus beta-actin gene: a useful tool for driving gene expression in vivo. Mol Biol Rep. 41:6817–6826. Co-first author
13. Li G, Feng J, Lei Y, Wang J, Wang H, Shang LK, Liü DT, Zhao H, Zhu Y and Wang YQ. (2014) Mutagenesis at specific genomic loci of amphioxus Branchiostoma belcheri using TALEN method. Journal of Genetics and Genomics. 41(4):215-219
14. Liü X, Li G, Feng J, Yang X, Wang YQ. (2013) An efficient microinjection method for unfertilized eggs of Asian amphioxus Branchiostoma belcheri. Dev Genes Evol. 223:269-278
15. Qian GH, Li G, Chen X, Wang YQ. (2013) Characterization and embryonic expression of four amphioxus Frizzled genes with important functions during early embryogenesis. Gene Expr Patterns. 13(8):445-453
16. Li G, Shu ZH and Wang YQ. (2013) Year-round reproduction and induced spawning of Chinese amphioxus, Branchiostoma Belcheri, in laboratory. PLoS ONE. 8(9): e75461
17. .Xu QH, Li G, Cao LX, Wang ZJ, Ye H, Chen XY, Yang X, Wang YQ and Chen LB. (2012) Proteomic characterization and evolutionary analyses of zona pellucida domain-containing proteins in the egg coat of the cephalochordate, Branchiostoma belcheri. BMC Evolutionary Biology. 12:239. Co-first author.
18. Li G, Yang X, Shu ZH, Chen XY and Wang YQ. (2012) Consecutive spawnings of Chinese amphioxus, Branchiostoma belcheri, in captivity. PLoS ONE 7(12): e50838
19. Li DL, Li G, Wang KR, Liu X, Li W, Chen XY, Wang YQ. (2012) Isolation and functional analysis of the promoter of the amphioxus Hsp70a gene. Gene. 510(1):39-46. Co-first author.
20. Li G, Holland PWH. (2010) The origin and evolution of ARGFX homeobox loci in mammalian radiation. BMC Evolutionary Biology. doi: 10.1186/1471-2148-10-182.
21. Li G, Zhang J, Sun Y, Wang H, Wang Y. (2009) The evolutionarily dynamic IFN-inducible GTPase proteins play conserved immune functions in vertebrates and cephalochordates. Mol Biol Evol. 26 (7):1619-1630.
22. Li G, Zhang QJ, Zhong J, Wang YQ. (2009) Evolutionary and functional diversity of green fluorescent proteins in cephalochordates. Gene. 446(1):41-49.
23. Li G, Shi P and Wang Y. (2007) Evolutionary dynamics of the ABCA-chr17q24 cluster genes in vertebrates. Genomics. 89 (3) 385–391.
24. Li G, Zhang QJ, Ji ZL, Wang YQ. (2007) Origin and evolution of vertebrate ABCA genes: A story from Amphioxus. Gene. 405 (1-2):88-95.
25. Zhang QJ, Li G, Sun Y, Wang YQ. (2009) Chromosome Preparation and Preliminary Observation of Two Amphioxus Species in Xiamen. Zoological Research. 30(2): 131?136