颜玉蓉
学术学位硕士生导师

教授



yanyurong1@sina.com

检验医学院





学科信息


个人简介
颜玉蓉，女，博士，教授，硕士研究生导师。

先后毕业于四川大学和重庆医科大学，获工学士学位和医学博士学位。1991年至今，于重庆医科大学检验医学院从事生物分析化学教学和科研工作。长期承担本科生的《卫生化学》、《化学分析与仪器分析》、《生物分析化学》课程的理论和实验教学，研究生的《蛋白质组学》课程的理论教学。
主持国家自然科学基金面上项目1项、重庆市科委项目3项、重庆市教委研究项目6项。共申请发明专利13项，其中授权6项。发表SCI论文32篇，EI论文3篇。共培养硕士研究生18名，其中毕业11名，在读7名。担任Trends Biotechnol.、Biosens. Bioelectron.、Chem. Commun.等高水平期刊审稿人。


主要研究方向为临床检验新技术的开发及应用，包括分子诊断技术、纳米生物技术和生物传感、活细胞成像技术等，主要涉及新型纳米材料的合成和应用、DNA纳米自组装、荧光和比色分析、电化学和电化学发光、表面等离子共振成像等。




论文情况

2021年
1.Cheng,X.^#,Yan, Y.^#,Chen, X., Duan, J., Zhang, D., Yang, T., Gou, X., Zhao, M., Ding, S., Cheng, W.*, 2021. CRISPR/Cas12a-Modulated fluorescence resonance energy transfer with nanomaterials for nucleic acid sensing, Sensors and Actuators B: Chemical, 2021, 331,129458.(IF=7.1)
2020年
1. Zhang, D.^#, Yan, Y.^#, Que, H., Yang, T., Cheng, X., Ding, S., Zhang, X., Cheng, W. ^*, 2020. CRISPR/Cas12a-Mediated Interfacial Cleaving of Hairpin DNA Reporter for Electrochemical Nucleic Acid Sensing. ACS Sens, 2020, 5: 557-562. (IF: 7.333)
2.Guo, B. ^#, Yan, Y.^#, Fan, L.; Wu, H.; Zhao, M.; Duan, X.; Cheng, W.; Ding, S^*, 2020. Molybdenum disulfide@5-carboxyfluorescein-probe biosensor for unamplified specific fragment detection in long nucleic acids based on magnetic composite probe-actuated deblocking of secondary structure. Anal Methods 2020, 12, 4813-4822.(IF: 2.596)
3.Han, D. ^#, Yan, Y. ^#, Bian, X.; Wang, J.; Zhao, M.; Duan, X.; Kong, L.; Cheng, W.; Ding, S^*, 2020. A novel electrochemical biosensor based on peptidoglycan and platinum-nickel-copper nano-cube for rapid detection of Gram-positive bacteria. Microchimica Acta 2020, 187. (IF: 6.232)
4.Liu, P. ^#, Qian, X. ^#, Li, X. ^#, Fan, L.; Li, X.; Cui, D.; Yan, Y^*, 2020. Enzyme-Free Electrochemical Biosensor Based on Localized DNA Cascade Displacement Reaction and Versatile DNA Nanosheets for Ultrasensitive Detection of Exosomal MicroRNA. ACS Interfaces 2020, 12, 45648-45656. (IF: 8.758)
5.Gan, X.; Han, D.; Wang, J.; Liu, P.; Li, X.; Zheng, Q.; Yan, Y^*, 2020. A highly sensitive electrochemiluminescence immunosensor for h-FABP determination based on self-enhanced luminophore coupled with ultrathin 2D nickel metal-organic framework nanosheets. Biosens Bioelectronics 2021, 171. (IF:10.257)


2019年
1. Que, H.^#, Zhang, D.^#, Guo, B., Wang, T., Wu, H., Han, D., Yan, Y.^*, 2019. Label-free and ultrasensitive electrochemical biosensor for the detection of EBV-related DNA based on AgDNCs@DNA/AgNCs nanocomposites and lambda exonuclease-assisted target recycling. Biosens Bioelectron 143, 111610. (IF: 10.257)
2. Wang, T.^#, Peng, Q. ^#, Guo B., Zhang, D., Zhao, M., Que, H., Wu, H., Yan, Y.^*, 2020. An integrated electrochemical biosensor based on target-triggered strand displacement amplification and "four-way" DNA junction towards ultrasensitive detection of PIK3CA gene mutation. Biosens Bioelectron 150, 111954. (IF:10.257)
3.Wang, T.^#, Que, H.^#, Cheng, W., Yan, X., Ma, H., Liu, P., Gan, X., Yan, Y.^*, 2019. Homogeneous fluorescent biosensing method for DNA methyltransferase activity analysis and inhibitor screening based on highly efficient isothermal amplification. Sensors and Actuators B: Chemical 296. (IF: 6.393)
4. Que, H.^#, Yan, X.^#, Guo, B.^#, Ma, H., Wang, T., Liu, P., Gan, X., Yan, Y.^*, 2019. Terminal Deoxynucleotidyl Transferase and Rolling Circle Amplification Induced G-triplex Formation: A Label-free Fluorescent Strategy for DNA Methyltransferase Activity Assay. Sensors and Actuators B: Chemical 291, 394-400. (IF: 6.393)
5. Fan, L.^#,Yan, Y.^#, Guo, B., Zhao, M., Li, J., Bian, X., Wu, H., Cheng, W., Ding, S.^*, 2019. Trimetallic hybrid nanodendrites and magnetic nanocomposites-based electrochemical immunosensor for ultrasensitive detection of serum human epididymis protein 4. Sensors and Actuators B: Chemical 296.(IF: 6.393)
6. Han, D.^#, Yan, Y.^#, Wang, J., Zhao, M., Duan, X., Kong, L., Wu, H., Cheng, W., Min, X., Ding, S.^*, 2019. An enzyme-free electrochemiluminesce aptasensor for the rapid detection of Staphylococcus aureus by the quenching effect of MoS₂-PtNPs-vancomycin to S₂O₈^2-/O₂ system. Sensors and Actuators B: Chemical 288, 586-593. (IF: 6.393)
7. Ma, H.^#, Guo, B.^#, Yan, X., Wang, T., Que, H., Gan, X., Liu, P., Yan, Y.^*, 2019. A smart fluorescent biosensor for the highly sensitive detection of BRCA1 based on a 3D DNA walker and ESDR cascade amplification. RSC Advances 9(34), 19347-19353. (IF: 3.049)


2018年
1. Yan, X.^#, Tang, M.^#, Yang, J., Diao, W., Ma, H., Cheng, W., Que, H., Wang, T., Yan, Y.^*, 2018. A one-step fluorescent biosensing strategy for highly sensitive detection of HIV-related DNA based on strand displacement amplification and DNAzymes. RSC Advances 8(55), 31710-31716.
2. Cheng, W.^#, Zhang, Y.^#, Yu, H., Diao, W., Mo, F., Wen, B., Cheng, W., Yan, Y.^*, 2018. An enzyme-free colorimetric biosensing strategy for ultrasensitive and specific detection of microRNA based on mismatched stacking circuits. Sensors and Actuators B: Chemical 255, 3298-3304.
3. Diao, W.^#, Tang, M.^#, Ding, S., Li, X., Cheng, W., Mo, F., Yan, X., Ma, H., Yan, Y.^*, 2018. Highly sensitive surface plasmon resonance biosensor for the detection of HIV-related DNA based on dynamic and structural DNA nanodevices. Biosens Bioelectron 100, 228-234.
2016年
1. Diao, W.^#, Tang, M.^#, Ding, X., Zhang, Y., Yang, J., Cheng, W., Mo, F., Wen, B., Xu, L., Yan, Y.^*, 2016. Electrochemical DNA biosensor based on MNAzyme-mediated signal amplification. Microchimica Acta 183(9), 2563-2569.
2. Yang, J.^#, Tang, M.^#, Diao, W., Cheng, W., Zhang, Y., Yan, Y.^*, 2016. Electrochemical strategy for ultrasensitive detection of microRNA based on MNAzyme-mediated rolling circle amplification on a gold electrode. Microchimica Acta 183(11), 3061-3067.
3. Yan, Y., Ding, S., Zhao, D., Yuan, R., Zhang, Y., Cheng, W.^*, 2016. Direct ultrasensitive electrochemical biosensing of pathogenic DNA using homogeneous target-initiated transcription amplification. Sci Rep 6, 18810.
4. Li, J., Lei, P., Ding, S., Zhang, Y., Yang, J., Cheng, Q., Yan, Y.^*, 2016. An enzyme-free surface plasmon resonance biosensor for real-time detecting microRNA based on allosteric effect of mismatched catalytic hairpin assembly. Biosens Bioelectron 77, 435-441.
5. Li, D., Cheng, W., Yan, Y., Zhang, Y., Yin, Y., Ju, H.^*, Ding, S.^*, 2016. A colorimetric biosensor for detection of attomolar microRNA with a functional nucleic acid-based amplification machine. Talanta 146, 470-476.
6. Yuan, R., Ding, S., Yan, Y., Zhang, Y., Zhang, Y., Cheng, W.^*, 2016. A facile and pragmatic electrochemical biosensing strategy for ultrasensitive detection of DNA in real sample based on defective T junction induced transcription amplification. Biosens Bioelectron 77, 19-25.
2015年
1. Wu, J.^#, Yan, Y.^#, Li, S., Ding, X., Ding, S.^*, Huang, Y.^*, Monitoring of patient glucose infusion using a surface plasmon resonance-based fiber optic sensor. Measurement Science & Technology, 2015, 26(10).
2. Chen, W.^#, Yan, Y.^#, Zhang, Y., Zhang, X., Yin, Y.^*, Ding, S.^*, 2015. DNA transducer-triggered signal switch for visual colorimetric bioanalysis. Sci Rep 5, 11190.
3. Li, S.^#, Yan, Y.^#, Zhong, L., Liu, P., Sang, Y., Cheng, W., Ding, S.^*, 2015. Electrochemical sandwich immunoassay for the peptide hormone prolactin using an electrode modified with graphene, single walled carbon nanotubes and antibody-coated gold nanoparticles. Microchimica Acta 182(11-12), 1917-1924.
4. Tao, C.^#, Yan, Y.^#, Xiang, H., Zhu, D., Cheng, W., Ju, H., Ding, S.^*, 2015. A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly. Chem Commun (Camb) 51(20), 4220-4222.
5. Zhang, Y.^#, Yan, Y.^#, Chen, W., Cheng, W., Li, S., Ding, X., Li, D., Wang, H., Ju, H., Ding, S.^*, 2015. A simple electrochemical biosensor for highly sensitive and specific detection of microRNA based on mismatched catalytic hairpin assembly. Biosens Bioelectron 68, 343-349.
6. Yan, Y., Shen, B., Wang, H., Sun, X., Cheng, W., Zhao, H., Ju, H., Ding, S.^*, 2015. A novel and versatile nanomachine for ultrasensitive and specific detection of microRNAs based on molecular beacon initiated strand displacement amplification coupled with catalytic hairpin assembly with DNAzyme formation. Analyst 140(16), 5469-5474.
7. Ding, X.^#, Yan, Y.^#, Li, S., Zhang, Y., Cheng, W., Cheng, Q., Ding, S.^*, 2015. Surface plasmon resonance biosensor for highly sensitive detection of microRNA based on DNA super-sandwich assemblies and streptavidin signal amplification. Anal Chim Acta 874, 59-65.
2014年
1. Lei, P.^#, Tang, H.^#, Ding, S., Ding, X., Zhu, D., Shen, B., Cheng, Q., Yan, Y.^*, 2014. Determination of the invA gene of Salmonella using surface plasmon resonance along with streptavidin aptamer amplification. Microchimica Acta 182(1-2), 289-296.
2. Li, Y.^#, Yan, Y.^#, Lei, Y., Zhao, D., Yuan, T., Zhang, D., Cheng, W., Ding, S.^*, 2014. Surface plasmon resonance biosensor for label-free and highly sensitive detection of point mutation using polymerization extension reaction. Colloids Surf B Biointerfaces 120, 15-20.
3. Zhu, D.^#, Yan, Y.^#, Lei, P., Shen, B., Cheng, W., Ju, H., Ding, S.^*, 2014. A novel electrochemical sensing strategy for rapid and ultrasensitive detection of Salmonella by rolling circle amplification and DNA-AuNPs probe. Anal Chim Acta 846, 44-50.
4. Shen, B.^#, Li, J.^#, Cheng, W.,Yan, Y., Tang, R., Li, Y., Ju, H., Ding, S.^*, 2014. Electrochemical aptasensor for highly sensitive determination of cocaine using a supramolecular aptamer and rolling circle amplification. Microchimica Acta 182(1-2), 361-367.
5. Cheng, W., Zhang, W.,Yan, Y., Shen, B., Zhu, D., Lei, P., Ding, S.^*, 2014. A novel electrochemical biosensor for ultrasensitive and specific detection of DNA based on molecular beacon mediated circular strand displacement and rolling circle amplification. Biosens Bioelectron 62, 274-279.


2013年
1. Yan, Y., Zhao, D., Yuan, T., Hu, J., Zhang, D., Cheng, W., Zhang, W., Ding, S.^*, 2013. A Simple and Highly Sensitive Electrochemical Biosensor for microRNA Detection Using Target-Assisted Isothermal Exponential Amplification Reaction. Electroanalysis, 25(10), 2354-2359.
2. Zhang, D.^#, Yan, Y.^#, Cheng, W., Zhang, W., Li, Y., Ju, H., Ding, S.^*, 2013. Streptavidin-enhanced surface plasmon resonance biosensor for highly sensitive and specific detection of microRNA. Microchimica Acta 180(5-6), 397-403.
2012年
1. Zhang, D.^#, Yan, Y.^#, Li, Q., Yu, T., Cheng, W., Wang, L., Ju, H., Ding, S.^*, 2012. Label-free and high-sensitive detection of Salmonella using a surface plasmon resonance DNA-based biosensor. J Biotechnol 160(3-4), 123-128.






知识产权
发明专利：
（1）基于滚环扩增和金纳米对沙门氏菌invA基因检测的方法，ZL0.7（已授权）；
（2）基于滚环扩增和超分子适体对可卡因电化学检测的方法，ZL1.0（已授权）；
（3）一种 microRNA 比色传感器的制备与应用，ZL9.1（已授权）；
（4）一种表面等离子体共振成像传感检测系统，ZL7.0（已授权）；
（5）一种电化学传感器及其制备与应用，ZL0.2（已授权）；
（6）一种流通池，ZL8.1（已授权）；
（7）一种表面等离子共振传感器及其制备与应用，ZL2.X（已授权）；
（8）一种检测 B 族链球菌的电化学传感器及其制 备与应用 ，ZL4.9(已授权）；
(9) 一种检测HIV相关基因的SPR传感器及其制备与应用，ZL0.6 （已授权）







著作教材
著作：
1.提高《化学分析与仪器分析》教学质量的探讨，重庆医学，2017，46(3):419-420。
2.研究生《蛋白质组学》样品制备与分离的教学探索，中华医学教育探索杂志，2012，11(12):1254-1256。







研究方向
分子诊断与生物分析